当前位置: X-MOL 学术Prog. Retin. Eye. Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Fluorescence lifetime imaging ophthalmoscopy.
Progress in Retinal and Eye Research ( IF 17.8 ) Pub Date : 2017-06-30 , DOI: 10.1016/j.preteyeres.2017.06.005
Chantal Dysli 1 , Sebastian Wolf 1 , Mikhail Y Berezin 2 , Lydia Sauer 3 , Martin Hammer 3 , Martin S Zinkernagel 1
Affiliation  

Imaging techniques based on retinal autofluorescence have found broad applications in ophthalmology because they are extremely sensitive and noninvasive. Conventional fundus autofluorescence imaging measures fluorescence intensity of endogenous retinal fluorophores. It mainly derives its signal from lipofuscin at the level of the retinal pigment epithelium. Fundus autofluorescence, however, can not only be characterized by the spatial distribution of the fluorescence intensity or emission spectrum, but also by a characteristic fluorescence lifetime function. The fluorescence lifetime is the average amount of time a fluorophore remains in the excited state following excitation. Fluorescence lifetime imaging ophthalmoscopy (FLIO) is an emerging imaging modality for in vivo measurement of lifetimes of endogenous retinal fluorophores. Recent reports in this field have contributed to our understanding of the pathophysiology of various macular and retinal diseases.

Within this review, the basic concept of fluorescence lifetime imaging is provided. It includes technical background information and correlation with in vitro measurements of individual retinal metabolites. In a second part, clinical applications of fluorescence lifetime imaging and fluorescence lifetime features of selected retinal diseases such as Stargardt disease, age-related macular degeneration, choroideremia, central serous chorioretinopathy, macular holes, diabetic retinopathy, and retinal artery occlusion are discussed. Potential areas of use for fluorescence lifetime imaging ophthalmoscopy will be outlined at the end of this review.



中文翻译:

荧光寿命成像检眼镜。

基于视网膜自发荧光的成像技术在眼科学中已发现了广泛的应用,因为它们非常敏感且无创。常规眼底自发荧光成像可测量内源性视网膜荧光团的荧光强度。它主要在视网膜色素上皮水平上从脂褐素衍生信号。然而,眼底自发荧光不仅可以通过荧光强度或发射光谱的空间分布来表征,而且可以通过特征性的荧光寿命函数来表征。荧光寿命是荧光团在激发后保持在激发态的平均时间。荧光寿命成像检眼镜(FLIO)是一种新兴的体内成像方式测量内源性视网膜荧光素的寿命。该领域的最新报道为我们对各种黄斑和视网膜疾病的病理生理学的理解做出了贡献。

在这篇综述中,提供了荧光寿命成像的基本概念。它包括技术背景信息以及与单个视网膜代谢物的体外测量相关的信息。在第二部分中,讨论了所选视网膜疾病的荧光寿命成像和荧光寿命特征的临床应用,这些疾病如Stargardt病,与年龄有关的黄斑变性,脉络膜疾病,中央浆液性脉络膜视网膜病变,黄斑裂孔,糖尿病性视网膜病变和视网膜动脉闭塞。荧光寿命成像检眼镜的潜在应用领域将在本评论的结尾处概述。

更新日期:2017-06-30
down
wechat
bug