Multivariate calibration coupled to RP-HPLC with diode array detection (HPLC-DAD) was applied to the identification and the quantitative evaluation of the short chain organic acids (malic, oxalic, formic, lactic, acetic, citric, pyruvic, succinic, tartaric, propionic and α-cetoglutaric) in fermented food. The goal of the present study was to get the successful resolution of a system in the combined occurrence of strongly coeluting peaks, of distortions in the time sensors among chromatograms, and of the presence of unexpected compounds not included in the calibration step. Second-order HPLC-DAD data matrices were obtained in a short time (10 min) on a C18 column with a chromatographic system operating in isocratic mode (mobile phase was 20 mmol L⁻¹ phosphate buffer at pH 2.20) and a flow-rate of 1.0 mL min⁻¹ at room temperature. Parallel factor analysis (PARAFAC) and unfolded partial least-squares combined with residual bilinearization (U-PLS/RBL) were the second-order calibration algorithms select for data processing. The performance of the analytical parameters was good with an outstanding limit of detection (LODs) for acids ranging from 0.15 to 10.0 mmol L⁻¹ in the validation samples. The improved method was applied to the analysis of many dairy products (yoghurt, cultured milk and cheese) and wine. The method was shown as an effective means for determining and following acid contents in fermented food and was characterized by reducibility with simple, high resolution and rapid procedure without derivatization of analytes.

多变量校准与带二极管阵列检测（RPD-HPLC）的RP-HPLC结合用于短链有机酸（苹果酸，草酸，甲酸，乳酸，乙酸，柠檬酸，丙酮酸，琥珀酸，酒石酸，丙酸和α-头戊二酸）。本研究的目的是在强共洗脱峰，色谱图中的时间传感器中的畸变以及校准步骤中未包括的意外化合物的组合出现中，获得系统的成功解析。在短时间内（10分钟）在具有等度模式（流动相为pH 2.20的20 mmol L ^-1磷酸盐缓冲液）运行的色谱系统的C18色谱柱上获得了二阶HPLC-DAD数据矩阵最小1.0毫升室温下为^-1。并行因子分析（PARAFAC）和展开的部分最小二乘结合残差双线性化（U-PLS / RBL）是用于数据处理的二阶校准算法。分析参数的性能良好，验证样品中酸的检测限（LOD）出色，为0.15至10.0 mmol L ^-1。改进的方法用于分析许多乳制品（酸奶，牛奶和奶酪）和葡萄酒。该方法被证明是测定和跟踪发酵食品中酸含量的有效手段，其特点是还原性好，具有简单，高分辨率和快速操作而无需衍生分析物的特点。