With the aim of pushing forward the limits of high efficient and ultrafast chiral liquid chromatography, a new Chiral Stationary Phase (CSP) has been prepared by covalently bonding the teicoplanin selector on 2.0 μm Superficially Porous Particles (SPPs). An already validated bonding protocol, which permits to achieve teicoplanin-based CSPs exhibiting zwitterionic behaviour, has been employed to prepare not only the 2.0 μm version of the CSP but also two other analogous CSPs based, respectively, on 2.7 μm SPPs and 1.9 μm Fully Porous Particles (FPPs). The kinetic performance of these CSPs has been compared through the analysis of both van Deemter curves and kinetic plots by employing in-house packed columns of 4.6 mm internal diameter and different lengths (20, 50 and 100 mm). In particular on the columns packed with 2.0 μm SPPs, extremely large efficiencies were observed for both achiral ( > 310,000 theoretical plates/meter, N/m; h_r: 1.61) and chiral compounds (>290,000 N/m; h_r: 1.72) in HILIC conditions. Thanks to their efficiency and enantioselectivity, these CSPs were successfully employed in ultrafast chiral separations. As an example, the enantiomers of haloxyfop were baseline resolved in about 3 s, with a resolution higher than 2.0, (flow rate: 8 mL/min) on a 2 cm long column packed with the 2.0 μm chiral SPPs.

为了突破高效和超快手性液相色谱的局限，通过将替考拉宁选择剂共价键合在2.0μm表面多孔颗粒（SPP）上，制备了一种新的手性固定相（CSP）。已经使用一种已经验证过的结合方案，该方案可以实现显示两性离子行为的基于替考拉宁的CSP，不仅用于制备2.0μm版本的CSP，而且还可以分别基于2.7μmSPP和1.9μmFully制备两种其他类似的CSP。多孔颗粒（FPP）。这些CSP的动力学性能已通过使用内部填充的4.6 mm内径和不同长度（20、50和100 mm）的填充柱，通过分析van Deemter曲线和动力学图进行了比较。特别是在装有2.0μmSPP的色谱柱上，N / m; h _r：1.61）和手性化合物（> 290,000 N / m; h _r：1.72）。由于它们的效率和对映选择性，这些CSP已成功用于超快手性分离。例如，在装满2.0μm手性SPP的2 cm长色谱柱上，在约3 s内对氟吡咯烷酮的对映异构体进行了基线拆分，分离度高于2.0（流速：8 mL / min）。