AbstractThe authors describe a voltammetric immunoassay for the hepatitis C virus core antigen. It is based on the use of vanadium oxide nanobelts that were used to modify a glassy carbon electrode (GCE). The nanobelts have a large specific surface area that can be heavily loaded with antibody against the hepatitis C virus core antigen. Following blocking with BSA and by using riboflavin as an electrochemical probe, the immuno GCE (best operated at a working voltage of −0.45 V vs. Ag/AgCl) has a linear response that covers the 10 fg·mL−1 to 100 ng·mL−1 core antigen concentration range and a limit of detection as low as 1.3 fg mL−1. Compared to the other redox probes, riboflavin is superior because it is oxidized in the negative potential range, where the number of interfering species for riboflavin is small. The method was successfully applied to the determination of hepatitis C virus core antigen in spiked serum. In our perception, the use of a V2O5 nanobelt film as a support for bioreceptors has a wide scope in that in that it may be extended to immunoassays for various other diagnostically significant analytes. Graphical abstractSchematic of a glassy carbon electrode modified with vanadium oxide nanobelts and immobilized antibody for electrochemical detection of the core antigen of Hepatitis C virus in serum samples. Riboflavin is used as an electrochemical probe.

中文翻译：

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氧化钒纳米带修饰玻碳免疫电极超灵敏伏安法测定丙型肝炎病毒核心抗原

摘要作者描述了丙型肝炎病毒核心抗原的伏安免疫测定。它基于用于修饰玻璃碳电极 (GCE) 的氧化钒纳米带的使用。纳米带具有很大的比表面积，可以装载大量针对丙型肝炎病毒核心抗原的抗体。用 BSA 封闭并使用核黄素作为电化学探针后，免疫 GCE（最佳工作电压为 -0.45 V 与 Ag/AgCl 相比）具有涵盖 10 fg·mL-1 至 100 ng·的线性响应mL-1 核心抗原浓度范围和低至 1.3 fg mL-1 的检测限。与其他氧化还原探针相比，核黄素更优越，因为它在负电位范围内被氧化，核黄素的干扰物质数量很少。该方法成功应用于加标血清中丙型肝炎病毒核心抗原的测定。在我们看来，使用 V2O5 纳米带薄膜作为生物受体的支持具有广泛的范围，因为它可以扩展到各种其他具有诊断意义的分析物的免疫测定。图解摘要用氧化钒纳米带和固定化抗体修饰的玻碳电极用于电化学检测血清样品中丙型肝炎病毒核心抗原的示意图。核黄素用作电化学探针。图解摘要用氧化钒纳米带和固定化抗体修饰的玻碳电极用于电化学检测血清样品中丙型肝炎病毒核心抗原的示意图。核黄素用作电化学探针。图解摘要用氧化钒纳米带和固定化抗体修饰的玻碳电极用于电化学检测血清样品中丙型肝炎病毒核心抗原的示意图。核黄素用作电化学探针。