The research in fluorescent organic nanoparticles (FONs) with aggregation-induced emission (AIE) feature shows an upward trend due to their outstanding optical properties and potential biomedical applications. In this work, a novel strategy has been developed for the first time through a catalyst-free azide-alkyne click reaction, which could directly conjugate azide containing polymers (PEGMA-AGE-N₃) and alkyne terminating AIE dye (named as PhE-OE) under mild experimental conditions. The final PEGMA-AGE-PhE copolymers containing AIE-active dye could self-assemble into FONs with intense fluorescence owing to their AIE feature. These PEGMA-AGE-PhE FONs were characterized by a series of characterization techniques in details. The cell viability as well as cell uptake behavior of PEGMA-AGE-PhE FONs was also examined to evaluate their potential for biomedical applications. We demonstrated that the catalyst-free azide-alkyne click reaction is effective for fabrication of AIE-active FONs and these AIE-active FONs showed high water dispersity and AIE feature. Moreover, the PEGMA-AGE-PhE FONs also exhibited low cytotoxicity and great potential for biological imaging. Taken together, a facile catalyst-free azide-alkyne click reaction with high efficiency has been developed for the preparation of AIE-active FONs, which showed excellent physicochemical properties for biological imaging applications.

具有聚集诱导发射（AIE）功能的荧光有机纳米颗粒（FON）的研究由于其出色的光学性能和潜在的生物医学应用而显示出上升的趋势。在这项工作中，首次通过无催化剂的叠氮化物-炔烃点击反应开发了一种新的策略，该反应可以直接偶联含叠氮化物的聚合物（PEGMA-AGE-N ₃）和炔烃终止AIE染料（称为PhE-OE）在温和的实验条件下。最终的含AIE活性染料的PEGMA-AGE-PhE共聚物由于具有AIE特性，可以自组装成具有强荧光的FON。这些PEGMA-AGE-PhE FONs通过一系列表征技术进行了详细表征。还检查了PEGMA-AGE-PhE FON的细胞生存力和细胞摄取行为，以评估其在生物医学应用中的潜力。我们证明了无催化剂的叠氮化物-炔烃点击反应对于AIE活性FON的制造是有效的，并且这些AIE活性FON表现出高的水分散性和AIE功能。此外，PEGMA-AGE-PhE FONs还显示出低细胞毒性和巨大的生物成像潜力。在一起