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Antibiotic Treatment Preventing Necrotising Enterocolitis Alters Urinary and Plasma Metabolomes in Preterm Pigs
Journal of Proteome Research ( IF 4.4 ) Pub Date : 2017-09-05 00:00:00 , DOI: 10.1021/acs.jproteome.7b00263
Pingping Jiang , Alessia Trimigno 1 , Jan Stanstrup , Bekzod Khakimov , Nanna Viereck , Søren Balling Engelsen , Per Torp Sangild , Lars Ove Dragsted
Affiliation  

Necrotising enterocolitis (NEC) is a serious gut inflammatory condition in premature neonates, onset and development of which depends on the gut microbiome. Attenuation of the gut microbiome by antibiotics can reduce NEC incidence and severity. However, how the antibiotics-suppressed gut microbiome affects the whole-body metabolism in NEC-sensitive premature neonates is unknown. In formula-fed preterm pigs, used as a model for preterm infants, plasma and urinary metabolomes were investigated by LC-MS and 1H-NMR, with and without antibiotic treatment immediately after birth. While reducing the gut microbiome density and NEC lesions as previously reported, the antibiotic treatment employed in the current study affected the abundance of 44 metabolites in different metabolic pathways. In antibiotics-treated pigs, tryptophan metabolism favoured the kynurenine pathway, relative to the serotonin pathway, as shown by specific metabolites. Metabolites associated with the gut microbiome, including 3-phenyllactic acid, 4-hydroxyphenylacetic acid and phenylacetylglycine, all from phenylalanine, and three bile acids showed lower levels in the antibiotics-treated pigs where the gut microbiome was extensively attenuated. Findings in the current study warrant further investigation of metabolic and developmental consequences of antibiotic treatment in preterm neonates.

中文翻译:

预防坏死性小肠结肠炎的抗生素治疗可改变早产猪的尿液和血浆代谢组

坏死性小肠结肠炎(NEC)是早产儿的一种严重的肠道炎症,其发作和发展取决于肠道微生物组。抗生素对肠道微生物组的影响可以降低NEC的发生率和严重程度。然而,未知抗生素抑制肠道微生物组如何影响NEC敏感早产儿的全身代谢。在用配方奶喂养的早产猪(作为早产儿的模型)中,通过LC-MS对血浆和尿液代谢组进行了研究,1出生后立即进行或不进行抗生素治疗的1 H-NMR。如先前报道,在降低肠道微生物组密度和NEC病变的同时,本研究中采用的抗生素治疗影响了不同代谢途径中44种代谢产物的丰度。如特定代谢物所示,相对于5-羟色胺途径,在用抗生素治疗的猪中,色氨酸代谢有利于犬尿氨酸途径。与肠道微生物组有关的代谢产物,包括3-苯乳酸,4-羟苯基乙酸和苯基乙酰基甘氨酸,全部来自苯丙氨酸,三种胆汁酸在经抗生素治疗的肠道微生物水平降低的猪中显示出较低的水平。
更新日期:2017-09-05
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