A novel series of un-metallized and metal complexes formazan dye (12–17) have been synthesized in an aqueous system, without using Buffers, with cost-effectiveness and improved fastness properties. The synthetic methodology involved the synthesis of 4-(2-(((2-hydroxy-5-nitrophenyl) diazenyl) (phenyl) methylene) hydrazinyl) benzene sulfonic acid followed by diazotization of 4-nitroamisnophenol which further coupled with coupler reagent i.e. 4-[(2Z)-2-benzylidenehydrazinyl] benzene sulfonic acid. Multichromic metal complexes of 4-(2-(((2-hydroxy-5-nitrophenyl) diazenyl) (phenyl) methylene) hydrazinyl) benzene sulfonic acid (1:1 and 2:1) were synthesized with the salts of Chromium, Iron, Cobalt, Copper and Nickel. Newly synthesized metal complexes were characterized by Ultraviolet–visible (UV–Vis), Fourier transforms infrared (FT-IR), Proton nuclear magnetic resonance (¹H NMR), C¹³-nuclear magnetic resonance (¹³C NMR), powder X-ray crystallography (XRD) and elemental analysis. Their performance as colorant material for different fastness properties like light fastness, wash fastness, perspiration fastness were evaluated on leather fabric and were shown to possess good fastness properties 3–5, 4–5, 3–5, and 4–5 respectively. The synthesized formazan dyes were also demonstrated the high percentage value of exhaustion and fixation on leather ranging from 91–97% and 90–98% respectively. The synthesized un-metallized and metal complex formazan dyes were ranged in color from Blue, violet, Green, Brown to Red. Antibacterial activity of synthesized formazan dyes was determined in solution by agar well diffusion method and on leather using the dyed leather fabric with synthesized dyes as model system. Maximum antibacterial activity in solution with ZOIs (19 ± 0.05 mm, 25 ± 0.07 mm, 23 ± 0.09 mm, 27 ± 0.03 mm) and significant % reduction in bacterial growth on leather (56 ± 0.03%, 65 ± 0.07%, 70 ± 0.05%, and 72 ± 0.08%) was demonstrated by chromium complex (15) against E. coli, S. aureus, Klebsiella and B. subtilis respectively.

一系列新颖的未金属化和金属配合物的甲dye染料（12-17）已在水性系统中合成，无需使用缓冲液，具有成本效益和改进的牢度特性。合成方法包括合成4-（2-（（（（2-羟基-5-硝基苯基）二氮烯基）（苯基）亚甲基）肼基）苯磺酸，然后重氮化4-硝基氨基苯酚，其进一步与偶联剂偶联，即4 -[[（2Z）-2-苄叉肼基]苯磺酸。用铬，铁的盐合成了4-（2-（（（（2-羟基-5-硝基苯基）二氮烯基）（苯基）亚甲基）肼基）苯磺酸（1：1和2：1）的多色金属配合物，钴，铜和镍。新合成的金属配合物的特征是紫外可见（UV-Vis），傅立叶变换红外（FT-IR），质子核磁共振（¹ H NMR），碳¹³核磁共振（¹³C NMR），粉末X射线晶体学（XRD）和元素分析。在皮革织物上评估了它们作为着色剂材料的不同牢度特性（如耐光牢度，耐洗牢度，耐汗牢度）的性能，并显示它们分别具有3-5、4-5、3-5和4-5的良好牢度。合成的甲maz染料在皮革上的耗竭和固色率也很高，分别为91-97％和90-98％。合成的非金属化和金属络合物的甲maz染料的颜色范围从蓝色，紫色，绿色，棕色到红色。通过琼脂井扩散法在溶液中测定合成的甲maz染料的抗菌活性，并使用以合成染料为模型的染色皮革织物在皮革上测定其抑菌活性。含ZOI的溶液中的最大抗菌活性（19±0。15）分别针对大肠杆菌，金黄色葡萄球菌，克雷伯菌和枯草芽孢杆菌。