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Scopulariopsis and scopulariopsis-like species from indoor environments
Studies in Mycology ( IF 16.5 ) Pub Date : 2017-03-18 , DOI: 10.1016/j.simyco.2017.03.001
J.H.C. Woudenberg , M. Meijer , J. Houbraken , R.A. Samson

Scopulariopsis-like species are often reported from the indoor environment, as well as from clinical samples. The lack of type isolates and thorough phylogenetic studies in the Microascaceae hampered the correct identification of these isolates. Based on recent phylogenetic studies, which resulted in multiple name changes, the aim is to molecularly identify the Scopulariopsis and scopulariopsis-like species which occur in the indoor environment and give an overview of the current species in these genera and their habitats. Strains from the CBS culture collection were supplemented with almost 80 indoor strains of which the internal transcribed spacer 1 and 2 and intervening 5.8S nrDNA (ITS), beta-tubulin (tub2) and translation elongation factor 1-alpha (tef1) gene regions were sequenced for phylogenetic inference. The multi-gene phylogenies recognise 33 Microascus species and 12 Scopulariopsis species and showed that the recently established genus Fuscoannellis, typified by Scopulariopsis carbonaria, should be synonymized with the genus Yunnania. Seven new Microascus species, four new Scopulariopsis species, and one new Yunnania species, are described, and a new name in Microascus and two new name combinations (one in Microascus, and one in Yunnania) are proposed. In the indoor environment 14 Microascus species and three Scopulariopsis species were found. Scopulariopsis brevicaulis (22 indoor isolates) and Microascus melanosporus (19 indoor isolates) are the most common indoor species, in number of isolates, followed by M. paisii (8 indoor isolates) and S. candida (7 indoor isolates). A genus phylogeny based on the ITS, tef1 and the large subunit 28S nrDNA (LSU) of the type or representative isolates of all here recognised species is provided depicting all species habitats. No correlation between phylogenetic relationship and habitat preference could be observed. Ten species which are found indoor are also found in relation with human-derived samples. A table showing recent name changes and a key to common species of Scopulariopsis and scopulariopsis-like genera found indoors is included.



中文翻译:

从室内环境和帚状物质

通常从室内环境以及临床样品中报告出类似镜猴的物种。在微as科中缺乏类型分离株和详尽的系统发育研究阻碍了对这些分离株的正确鉴定。根据最近的系统发育研究,这导致了多个名称的改变,其目的是分子识别发生在室内环境和帚样的物种,并给这些属和它们的栖息地目前物种的概述。来自CBS培养物收集的菌株补充了近80种室内菌株,其内部转录的间隔物1和2以及插入的5.8S nrDNA(ITS),β-微管蛋白(tub2)和翻译延伸因子1-alpha(对tef1)基因区域进行测序,以进行系统发育推断。多基因系统发育承认33个Microascus种和12个物种和显示,最近成立的属Fuscoannellis,由代表帚carbonaria,应与被属异名Yunnania。七个新Microascus物种,四个新帚霉物种,以及一个新Yunnania物种,被描述,并且在一个新名称Microascus和两个新的名称的组合(一个在Microascus,和一个在Yunnania)。在室内环境14个Microascus种和三个帚霉种被发现。短帚霉(22室内株)和Microascus melanosporus(19室内株)是最常见的室内物种,在分离物的数目,其次是M. paisii(8室内株)和S.念珠菌(7室内株)。基于ITS tef1的属系统发育提供了所有此处公认物种的类型或代表性分离物的大亚基28S nrDNA(LSU),描绘了所有物种的栖息地。系统发育关系和栖息地偏好之间没有相关性。在室内也发现了十种与人类来源的样​​本有关的物种。最近显示名称更改,并共同种关键的表帚霉和帚状属室内发现包括。

更新日期:2017-03-18
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