A culture-based survey of staining fungi on oil-treated timber after outdoor exposure in Australia and the Netherlands uncovered new taxa in Pezizomycotina. Their taxonomic novelty was confirmed by phylogenetic analyses of multi-locus sequences (ITS, nrSSU, nrLSU, mitSSU, RPB1, RPB2, and EF-1α) using multiple reference data sets. These previously unknown taxa are recognised as part of a new order (Superstratomycetales) potentially closely related to Trypetheliales (Dothideomycetes), and as a new species of Cyanodermella, C. oleoligni in Stictidaceae (Ostropales) part of the mostly lichenised class Lecanoromycetes. Within Superstratomycetales a single genus named Superstratomyces with three putative species: S. flavomucosus, S. atroviridis, and S. albomucosus are formally described. Monophyly of each circumscribed Superstratomyces species was highly supported and the intraspecific genetic variation was substantially lower than interspecific differences detected among species based on the ITS, nrLSU, and EF-1α loci. Ribosomal loci for all members of Superstratomyces were noticeably different from all fungal sequences available in GenBank. All strains from this genus grow slowly in culture, have darkly pigmented mycelia and produce pycnidia. The strains of C. oleoligni form green colonies with slimy masses and develop green pycnidia on oatmeal agar. These new taxa could not be classified reliably at the class and lower taxonomic ranks by sequencing from the substrate directly or based solely on culture-dependent morphological investigations. Coupling phenotypic observations with multi-locus sequencing of fungi isolated in culture enabled these taxonomic discoveries. Outdoor situated timber provides a great potential for culturable undescribed fungal taxa, including higher rank lineages as revealed by this study, and therefore, should be further explored.

在澳大利亚和荷兰进行户外暴露后，一项基于文化的对油处理过的木材上的染色真菌的调查发现，Pezizomycotina中发现了新的分类单元。通过使用多个参考数据集对多基因座序列（ITS，nrSSU，nrLSU，mitSSU，RPB1，RPB2和EF-1α）进行系统发育分析，证实了它们的分类学新颖性。这些先前未知的分类单元被认为是可能与Trypetheliales（Dothideomycetes）密切相关的新秩序（Superstratomycetales）的一部分，并被认为是Stictidaceae（Ostropales）中的Cyanodermella，C。）大部分为地衣化类Lecanoromycetes。在Superstratomycetales同一个属命名Superstratomyces三个推定物种：S. flavomucosus，S. atroviridis和S. albomucosus被正式描述。每个单系外接Superstratomyces物种高度支持和种内遗传变异基本上低于种间差异基于所述ITS，nrLSU物种中检测，和EF-1α基因座。超级纹癣菌所有成员的核糖体基因座与GenBank中所有可用的真菌序列明显不同。该属的所有菌株在培养中生长缓慢，色素沉着的菌丝体较深，并产生粘虫。油菜梭菌菌株形成糊状团块的绿色菌落，并在燕麦琼脂上形成绿色粘虫。通过直接从底物进行测序或仅基于依赖于培养物的形态学研究，无法将这些新的分类单元可靠地分类到该分类和较低的分类学等级上。表型观察与文化中分离的真菌的多基因座测序相结合，实现了这些分类学发现。位于室外的木材为可培养的未描述真菌类群提供了巨大潜力，包括该研究揭示的更高等级的谱系，因此应进一步探索。