The present work represents the ultrasound assisted synthesis of citronellol laurate using Novozym 435 as a biocatalyst in a solvent free condition. The reaction was evaluated by two methods, one factor at a time and central composite design (CCD) by RSM. Initially, the first method produced maximum conversion up to 93.36% using optimal conditions (1:4 acid to alcohol ratio, 2% enzyme loading, 60 °C temperature, 300 rpm, 80 W power, 50% duty cycle and 25 kHz frequency). After estimation of parameters, the experimental design expert was used to evaluate the effect of molar ratio, enzyme loading, duty cycle and ultrasound power on the citronellol laurate synthesis. The optimal conditions for the ultrasound assisted synthesis of citronellol laurate was found to be: molar ratio of 1:4.2 lauric acid to citronellol, enzyme loading 2.11, ultrasound power 83.85 W and 48.54% duty cycle. Under these conditions, it was possible to obtain 96.43% of conversion in a shorter time (40 min). The Novozym 435 could be recycled in 5 successive reaction cycles. The Ordered Bi Bi kinetic model has well fitted for ultrasound assisted synthesis of citronellol laurate.

本工作代表了在无溶剂条件下使用Novozym 435作为生物催化剂的超声辅助月桂酸香茅醇的合成。通过两种方法评估反应，一次评估一个因素，然后通过RSM进行中心复合设计（CCD）。最初，第一种方法在最佳条件下（酸与酒精的比例为1：4、2％的酶负载，60°C的温度，300 rpm，80 W的功率，50％的占空比和25 kHz的频率）产生的最大转化率高达93.36％。 。在估计参数之后，使用实验设计专家来评估摩尔比，酶负载，占空比和超声功率对月桂酸香茅醇合成的影响。发现超声辅助合成月桂酸香茅醇的最佳条件为：月桂酸与香茅醇的摩尔比为1：4.2，酶负荷为2.11，超声功率为83.85 W，占空比为48.54％。在这些条件下，有可能在更短的时间内（40分钟）获得96.43％的转化率。Novozym 435可以在5个连续的反应循环中循环使用。Ordered Bi Bi动力学模型非常适合于月桂酸香茅醇的超声辅助合成。