The goal of this study was to develop a method that utilized a hydrophilic C18 stationary phase in the preparative high performance liquid chromatography to isolate the polar part from the rhizomes of Anemarrhena asphodeloides. The results showed that an initial mobile phase of pure water for the separation could greatly increase the retention and solubility of the polar compounds at the preparative scale. Introducing polar groups on the surface of the hydrophilic C18 column together with the use of optimized mobile phase compositions improved the column separation selectivity for polar compounds. Eleven previously undescribed compounds in Anemarrhena asphodeloides were obtained, indicating that the method developed in this study would facilitate the purification and separation of the polar part of traditional Chinese medicines.

本研究的目的是开发一种在制备型高效液相色谱中利用亲水性C18固定相分离知母根茎中极性部分的方法。结果表明，用于分离的纯净水的初始流动相可以大大提高极性化合物在制备规模上的保留度和溶解度。在亲水性C18色谱柱表面上引入极性基团，以及使用优化的流动相组成，可改善极性化合物的色谱柱分离选择性。知母二十倍体中的11种先前未描述的化合物 结果表明，本研究开发的方法将有助于中药极性部分的纯化和分离。