Coupling of asymmetric flow field-flow fractionation (AF4) to an on-line elemental detection (inductively coupled plasma-mass spectrometry, ICP-MS) has been recently proposed as a powerful diagnostic tool for characterization of the bioconjugation of CdSe/ZnS core-shell Quantum Dots (QDs) to antibodies. Such approach has been used herein to demonstrate that cap exchange of the native hydrophobic shell of core/shell QDs with the bidentate dihydrolipoic acid ligands directly removes completely the eventual side nanoparticulated populations generated during simple one-pot synthesis, which can ruin the subsequent final bioapplication. The critical assessment of the chemical and physical purity of the surface-modified QDs achieved allows to explain the transmission electron microscopy findings obtained for the different nanoparticle surface modification assayed.

最近，非对称流场流分离（AF4）与在线元素检测（电感耦合等离子体质谱，ICP-MS）的耦合已被提议作为表征CdSe / ZnS核生物共轭特性的强大诊断工具。外壳的抗体量子点（QD）。本文已使用这种方法来证明核/壳QD的天然疏水壳与双齿二氢硫辛酸配体的帽交换直接完全去除了在简单的一锅合成过程中产生的最终的侧纳米颗粒种群，这可能会破坏后续的最终生物应用。