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Mass spectrometry-based metabolomics to assess uptake of silver nanoparticles by Arabidopsis thaliana
Environmental Science: Nano ( IF 7.3 ) Pub Date : 2017-08-31 00:00:00 , DOI: 10.1039/c7en00555e
Nita G. Chavez Soria 1, 2, 3, 4, 5 , Angelina Montes 1, 2, 3, 4, 5 , Mary A. Bisson 2, 3, 4, 5, 6 , G. Ekin Atilla-Gokcumen 1, 2, 3, 4, 5 , Diana S. Aga 1, 2, 3, 4, 5
Affiliation  

Silver nanoparticles (AgNPs) are one of the most commonly utilized engineered nanomaterials in consumer products due to their antimicrobial properties. AgNPs can be introduced into the environment when products containing AgNPs are disposed of in landfills, or leach out from products during washing, eventually reaching wastewater treatment plants. Hence, the usage of reclaimed water can lead to the unintentional exposure of different biological organisms to AgNPs. In addition, biosolids from wastewater treatment plants may contain AgNPs that can end up in the environment when the biosolids are land-applied to fertilize fields. In this study, the impact of citrate- and polyvinylpyrolidone-coated AgNPs on a model plant, Arabidopsis thaliana, was investigated using hydroponic cultures to quantitatively evaluate plant uptake of AgNPs, and to demonstrate the use of a metabolomics approach in assessing sub-lethal effects of AgNPs on plants. Analysis of AgNPs was performed using inductively coupled plasma mass spectrometry (ICP-MS) to quantify total silver concentrations in plant tissues, and metabolite profiling was achieved using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QToF/MS). Results show silver translocation from roots of Arabidopsis thaliana to leaves and flowering shoots in all plants treated with AgNPs. Furthermore, untargeted and targeted metabolomics results show alterations in the metabolome after treatment, as well as the accumulation of specific phytosphingosines and N-acylethanolamines in silver treated Arabidopsis thaliana. Correlating the silver concentrations in Arabidopsis thaliana and the changes in metabolite profile may provide insights on the mechanism and interactions between nanomaterials and biological organisms. This study lays the groundwork and provides a proof-of-concept on the utilization of mass spectrometry-based metabolomics as a tool for determining the biochemical effects of nanoparticles at sub-lethal concentrations in Arabidopsis thaliana.

中文翻译:

基于质谱的代谢组学,评估拟南芥对银纳米颗粒的吸收

银纳米颗粒(AgNPs)由于其抗菌特性,是消费产品中最常用的工程纳米材料之一。当含有AgNPs的产品被丢弃在垃圾填埋场中时,AgNPs可能会被引入环境中,或者在洗涤过程中会从产品中浸出,最终到达废水处理厂。因此,再生水的使用可能导致不同生物有机体意外暴露于AgNPs。此外,来自废水处理厂的生物固体中可能含有AgNPs,当这些生物固体在土地上施肥以肥田时,它们可能会最终进入环境。在这项研究中,柠檬酸盐和聚乙烯吡咯烷酮包覆的AgNP对模型植物拟南芥的影响进行了水培培养,以定量评估植物对AgNPs的吸收,并证明了利用代谢组学方法评估AgNPs对植物的亚致死作用。使用感应耦合等离子体质谱(ICP-MS)进行AgNPs分析以定量植物组织中的总银浓度,并使用液相色谱四极杆飞行时间质谱(LC-QToF / MS)实现代谢物谱分析。结果显示,在所有用AgNPs处理的植物中,银从拟南芥的根向叶和花梢移位。此外,未靶向和靶向代谢组学结果显示,治疗后代谢组发生了变化,以及特定的植物鞘氨醇和的积累银处理的拟南芥中的β-酰基乙醇胺。将拟南芥中的银浓度与代谢物谱的变化相关联,可以提供有关纳米材料与生物有机体之间的机理和相互作用的见解。这项研究奠定了基础,并为基于质谱的代谢组学作为确定拟南芥亚致死浓度下纳米颗粒的生化效应的工具提供了概念验证。
更新日期:2017-08-31
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