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Nitrogen-doped graphene quantum dot for direct fluorescence detection of Al3+ in aqueous media and living cells
Biosensors and Bioelectronics ( IF 12.6 ) Pub Date : 2017-08-26 , DOI: 10.1016/j.bios.2017.08.057
Bi-Yun Fang , Cheng Li , Yuan-Yang Song , Fang Tan , Yuan-Cheng Cao , Yuan-Di Zhao

Graphene quantum dot (GQD) has been attractive in analytical science field due to its low toxicity, stable photoluminescence. Herein, nitrogen-doped GQD (N-GQD) was prepared by a facile solvothermal treatment of GO using dimethylformamide, and exhibited a green emission with 23.1% quantum yield. The N-GQD probe showed a selective and sensitive fluorescence enhancement response to Al3+, the mechanism might be the formation of a complex between Al3+ and N-GQD constrained the photo-induced electron transfer (PET) process of N-GQD itself. With Benesi-Hildebrand equation, the binding constant and molar ratio between N-GQD and Al3+ was calculated to be 4.6 × 104 L mol−1 and 1:1 respectively. The pKa value of N-GQD was also determined to be 4.4 by capillary electrophoresis. In pH 4.0 PBS solution, there was a good linear relation between the fluorescence intensity and the logarithm of concentration of Al3+ in the range of 2.5–75 μmol L−1, the limit of detection (3σ) was 1.3 μmol L−1. This “Off - On” fluorescence method had been applied to accurate quantification of aluminum in hydrotalcite tablets. What's more, the fluorescence switch property of N-GQD was explored by alternate addition of Al3+ and EDTA. The probe was also utilized for detection Al3+ in living cells due to its excellent biocompatibility.



中文翻译:

氮掺杂石墨烯量子点,用于直接荧光检测水性介质和活细胞中的Al 3+

石墨烯量子点(GQD)由于其低毒性,稳定的光致发光而在分析科学领域颇具吸引力。在此,通过使用二甲基甲酰胺对GO进行轻松的溶剂热处理来制备氮掺杂的GQD(N-GQD),并显示出绿色发射,量子产率为23.1%。N-GQD探针显示出对Al 3+的选择性和敏感的荧光增强反应,其机制可能是Al 3+与N-GQD之间形成复合物,限制了N-GQD的光诱导电子转移(PET)过程本身。利用Benesi-Hildebrand方程,计算出N-GQD与Al 3+的结合常数和摩尔比分别为4.6×10 4  L mol -1和1:1。在P ķ通过毛细管电泳也将N-GQD值确定为4.4。在pH 4.0 PBS溶液中,荧光强度与Al 3+浓度的对数在2.5–75μmolL -1之间具有良好的线性关系,检出限(3σ)为1.3μmolL -1。这种“开-关”荧光方法已应用于水滑石片中铝的准确定量。此外,通过交替添加Al 3+和EDTA探索了N-GQD的荧光开关特性。该探针具有出色的生物相容性,因此也可用于检测活细胞中的Al 3+

更新日期:2017-08-26
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