For more than two decades, mass spectrometry has been a central technology for proteomics research. During this time, the use of chemical reactions to introduce tags for quantification, affinity enrichment and other uses has facilitated the comprehensive profiling of the proteome by mass spectrometry in many ways. In the last decade, the introduction of more powerful instruments and more sophisticated data acquisition and data analysis routines has made some strategies obsolete, while making other approaches practically feasible only now. Here, the current status of chemical tagging strategies in proteomics is reviewed, with a particular emphasis on proteome quantification workflows, strategies for the enrichment of (post-translationally) modified proteins and peptides, and methods for structural proteomics.

中文翻译：

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化学修饰方法在当代基于质谱的蛋白质组学研究中的作用综述

二十多年来，质谱法一直是蛋白质组学研究的核心技术。在此期间，使用化学反应引入用于定量、亲和富集和其他用途的标签在许多方面促进了通过质谱法对蛋白质组进行全面分析。在过去十年中，更强大的仪器和更复杂的数据采集和数据分析程序的引入已经使一些策略过时了，而其他方法直到现在才切实可行。在这里，回顾了蛋白质组学中化学标记策略的现状，特别强调了蛋白质组量化工作流程、（翻译后）修饰的蛋白质和肽的富集策略以及结构蛋白质组学的方法。