8-Hydroxydeoxyguanosine (8-OHdG) is a widely-used biomarker of oxidative DNA damages. 8-OHdG in peripheral blood leukocyte is associated with mutation and cancer risk. The level of 8-OHdG in peripheral blood leukocytes can indicate a long-term response to oxidative stress rather than that in urine. Accurate identification and quantification of leukocyte 8-OHdG are essential for understanding its mechanism of formation, repair, and biological consequences. In this study, a fast and accurate ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS) method was developed and validated to detect 8-OHdG in human peripheral leukocyte. DNA in blood samples were extracted and digested, then subjected onto UHPLC–MS/MS using an isocratic elution on a Zorbax Eclipse Plus C18 column (2.1 × 100 mm, 1.8 μm). Multiple reaction monitoring (MRM) mode was adopted by using [¹⁵N₅]-8-OHdG as an internal standard. The assay was linear over the concentration range of 1.0–100 nM with R² = 0.999. The accuracy for spiked samples was 90.9%–94.8%, and the intra-day precision was within 3.7%. The limit of detection (LOD) is 0.30 nM and limit of quantification (LOQ) is 1.0 nM with 5 μl of sample injection. By the analysis of human leukocyte 8-OHdG (n = 121) using the developed UHPLC–MS/MS method, it demonstrates that the level of leukocyte 8-OHdG of the cancer patients group (n = 46) is significantly higher than that of the health control (n = 75).

8-羟基脱氧鸟苷（8-OHdG）是氧化DNA损伤的广泛使用的生物标志物。外周血白细胞中的8-OHdG与突变和癌症风险有关。外周血白细胞中8-OHdG的水平可以表明对氧化应激的长期反应，而不是尿液中的反应。准确鉴定和定量白细胞8-OHdG对于了解其形成，修复和生物学后果的机制至关重要。在这项研究中，开发了一种快速，准确的超高效液相色谱-串联质谱（UHPLC–MS / MS）方法，并验证了该方法可检测人外周血白细胞中的8-OHdG。提取并消化血样中的DNA，然后在Zorbax Eclipse Plus C18色谱柱（2.1×100 mm，1.8μm）上使用等度洗脱进行UHPLC-MS / MS处理。¹⁵ N ₅ ] -8-OHdG作为内标。该测定在1.0-100 nM的浓度范围内呈线性关系，R ²  = 0.999。加标样品的准确度为90.9％至94.8％，日内准确度在3.7％以内。进样量为5μl时，检测限（LOD）为0.30 nM，定量限（LOQ）为1.0 nM。通过使用发达的UHPLC-MS / MS方法分析人白细胞8-OHdG（n = 121），表明癌症患者组（n = 46）的白细胞8-OHdG水平明显高于人白细胞8-OHdG（n = 46）。健康控制（n = 75）。