Six new iridoid glycosides, sambucusides A–F (1–6), and two known derivatives (7 and 8) were isolated from a methanol extract of the twigs of Sambucus williamsii var. coreana. Their chemical structures were elucidated by spectroscopic methods, including NMR (¹H and ¹³C NMR, ¹H–¹H COSY, HMQC, HMBC, and NOESY) and HRMS. All isolated compounds (1–8) were evaluated for their antiproliferative activities against four human cancer cell lines (A549, SK-OV-3, SK-MEL-2, and Bt549). Their effects on nitric oxide (NO) production in lipopolysaccharide (LPS)-activated BV-2 cells and their neuroprotective effects through induction of nerve growth factor (NGF) in C6 glioma cells were also examined. Compounds 2, 3, and 5 showed cytotoxic effects (IC₅₀ 1.3–8.7 μM) against the SK-MEL-2 and Bt549 cell lines and inhibitory effects on NO production (IC₅₀ of 0.9, 1.3, and 1.2 μM, respectively). Compounds 2, 4, and 8 exhibited NGF-releasing effects (147.0 ± 5.8%, 158.7 ± 5.2%, and 152.6 ± 7.3%, respectively).

中文翻译：

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从树枝环烯醚萜苷接骨木变种。科纳纳及其生物活性

六个新的环烯醚萜苷，sambucusides A-F（1 - 6），以及两个公知的衍生物（7和8）从的树枝的甲醇提取物中分离接骨木变种。科里亚纳。通过光谱方法阐明了它们的化学结构，包括NMR（¹ H和¹³ C NMR，¹ H- ¹ H COSY，HMQC，HMBC和NOESY）和HRMS。所有分离的化合物（1 – 8评估了它们对四种人类癌细胞系（A549，SK-OV-3，SK-MEL-2和Bt549）的抗增殖活性。还检查了它们对脂多糖（LPS）激活的BV-2细胞中一氧化氮（NO）产生的影响，以及通过诱导C6胶质瘤细胞中神经生长因子（NGF）诱导的神经保护作用。化合物2，3和5个显示出细胞毒性作用（IC ₅₀（IC靠在NO产生的SK-MEL-2和BT549细胞系和抑制作用1.3-8.7μM）₅₀ 0.9，1.3和1.2μM，分别地）。化合物2，4，和8 表现出NGF释放作用（分别为147.0±5.8％，158.7±5.2％和152.6±7.3％）。