A PEG-based cyclized vinyl polymer was synthesized via one-step RAFT polymerization and used as a precursor of injectable hydrogels. Dithiol linkers including laminin-derived peptides containing IKVAV and YIGSR sequences and DTT were used for gelation. Fast and adjustable gelation rate was achieved through nucleophile-initiated thiol-Michael reaction under physiological conditions. Low swelling ratio and moderate degradation rate of the formed hydrogels were observed. 3D encapsulation of neural progenitor cells in the synthetic hydrogel showed good cell viability over 8 days. The long-term cell survival and proliferation were promoted by the introduction of laminin-derived peptides. This hydrogel platform based on peptide-cross-linked, cyclized vinyl polymers can be used as a universal hydrogel template for 3D cell encapsulation.

中文翻译：

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基于肽交联的环状聚合物纳米粒子的可调节，可注射水凝胶，可用于神经祖细胞的递送。

通过一步RAFT聚合合成了基于PEG的环化乙烯基聚合物，并将其用作可注射水凝胶的前体。使用二硫醇接头（包括层粘连蛋白衍生的含有IKVAV和YIGSR序列的肽）和DTT进行凝胶化。在生理条件下，通过亲核试剂引发的巯基-迈克尔反应，可实现快速且可调节的胶凝速率。观察到形成的水凝胶的低溶胀率和适度的降解速率。在合成水凝胶中神经祖细胞的3D封装在8天中显示出良好的细胞活力。层粘连蛋白衍生肽的引入促进了长期细胞存活和增殖。基于肽交联的环化乙烯基聚合物的这种水凝胶平台可用作3D细胞封装的通用水凝胶模板。