Two dimeric clerodane diterpene glycosides, namely, bistinospinosides A (1) and B (2), were isolated from the roots of Tinospora sagittata. Their structures were elucidated by extensive spectroscopic data interpretation. The compounds feature an unusual 1,4-epoxycyclohexane ring in their structures and may be biosynthetically constructed via an intermolecular Diels–Alder [4+2] cycloaddition from the corresponding clerodane diterpene. The compounds were evaluated in a nitric oxide inhibitory assay using J774.1 macrophage-like cells.

中文翻译：

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Bistinospinosides A和B，二倍体的Clerodane二萜糖苷，来自箭叶（Tinospora sagittata）

两个二聚的二萜克罗糖苷，即，bistinospinosides A（1）和B（2），从根分离青牛胆sagittata的。通过广泛的光谱数据解释阐明了它们的结构。这些化合物在其结构中具有一个不寻常的1,4-环氧环己烷环，并且可以通过分子间Diels-Alder [4 + 2]环加成反应从相应的环戊二烯双萜中生物合成。使用J774.1巨噬细胞样细胞在一氧化氮抑制测定中评估化合物。