We have developed a cost-effective and portable graphene-enabled biosensor to detect Zika virus with a highly specific immobilized monoclonal antibody. Field Effect Biosensing (FEB) with monoclonal antibodies covalently linked to graphene enables real-time, quantitative detection of native Zika viral (ZIKV) antigens. The percent change in capacitance in response to doses of antigen (ZIKV NS1) coincides with levels of clinical significance with detection of antigen in buffer at concentrations as low as 450 pM. Potential diagnostic applications were demonstrated by measuring Zika antigen in a simulated human serum. Selectivity was validated using Japanese Encephalitis NS1, a homologous and potentially cross-reactive viral antigen. Further, the graphene platform can simultaneously provide the advanced quantitative data of nonclinical biophysical kinetics tools, making it adaptable to both clinical research and possible diagnostic applications. The speed, sensitivity, and selectivity of this first-of-its-kind graphene-enabled Zika biosensor make it an ideal candidate for development as a medical diagnostic test.

我们已经开发了一种具有成本效益的便携式石墨烯生物传感器，可以用高度特异性的固定化单克隆抗体检测寨卡病毒。具有与石墨烯共价连接的单克隆抗体的场效应生物传感（FEB）可以实时，定量检测天然Zika病毒（ZIKV）抗原。响应抗原剂量（ZIKV NS1）的电容变化百分数与临床意义水平相符，检测浓度低至450 pM的缓冲液中的抗原。通过在模拟人血清中测量Zika抗原，证明了潜在的诊断应用。使用日本脑炎NS1（一种同源且可能交叉反应的病毒抗原）验证了选择性。进一步，石墨烯平台可同时提供非临床生物物理动力学工具的高级定量数据，使其既适用于临床研究，又适用于可能的诊断应用。这种首创的启用石墨烯的Zika生物传感器的速度，灵敏度和选择性使其成为开发作为医学诊断测试的理想候选者。