AbstractThe authors describe an array for chemiluminescence (CL) based determination of cardiac troponin T (cTnT), an important cardiovascular disease marker. The tracing tag consists of silver nanoparticles (AgNPs) loaded with guanine-rich DNA sequences and detection antibody in a high numerical ratio. The loaded AgNPs were then reacted with hemin to form a hemin/G-quadruplex DNAzyme. A disposable immunosensor array was fabricated by immobilizing capture antibody on corresponding sensing sites on a glass chip. Once a sandwich immunocomplex is formed on the array, the tracing tag catalyzes the CL reaction of the luminol-p-iodophenol and H2O2 system to produce a CL signal, which is collected by a CCD camera. An intuitive CL image is obtained containing all of the spots on the array. Under optimal conditions, the method shows a wide linear range over 4 orders of magnitude (from 0.003 to 270 ng·L−1), a detection limit down to 84 fg·L−1, and a throughput as high as 44 tests·h−1. The results obtained with serum samples are in acceptable agreement with reference values. The AgNP-based tracing tag as well as the immunoassay method shows a promising potential for point-of-care testing for the early clinical diagnosis of cardiovascular disease. Graphical abstractSchematic presentation of silver nanoparticles (AgNPs) functionalized with hemin/G-quadruplex DNAzyme for highly sensitive chemiluminescence (CL) immunoassay of cardiac troponin T (cTnT) on a glass chip array.

中文翻译：

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通过在玻璃芯片阵列上使用氯化血红素/G-四链体 DNAzyme 功能化的银纳米粒子对心肌肌钙蛋白 T 进行化学发光免疫测定

摘要作者描述了基于化学发光 (CL) 测定心肌肌钙蛋白 T (cTnT) 的阵列，这是一种重要的心血管疾病标志物。追踪标签由以高数值比加载富含鸟嘌呤的 DNA 序列和检测抗体的银纳米粒子 (AgNP) 组成。然后将负载的 AgNPs 与氯化血红素反应形成氯化血红素/G-四链体 DNAzyme。一次性免疫传感器阵列是通过将捕获抗体固定在玻璃芯片上的相应传感位点上来制造的。一旦在阵列上形成夹心免疫复合物，示踪标签就会催化鲁米诺-对碘苯酚和 H2O2 系统的 CL 反应产生 CL 信号，该信号由 CCD 相机收集。获得包含阵列上所有点的直观 CL 图像。在最佳条件下，该方法显示出超过 4 个数量级的宽线性范围（从 0.003 到 270 ng·L-1），检测限低至 84 fg·L-1，吞吐量高达 44 次测试·h-1。用血清样品获得的结果与参考值一致。基于 AgNP 的示踪标签以及免疫测定方法显示了用于心血管疾病早期临床诊断的即时检测的潜力。图形摘要 用血红素/G-四链体脱氧核糖核酸功能化的银纳米粒子 (AgNP) 的示意图，用于在玻璃芯片阵列上对心肌肌钙蛋白 T (cTnT) 进行高灵敏度化学发光 (CL) 免疫测定。用血清样品获得的结果与参考值一致。基于 AgNP 的示踪标签以及免疫测定方法显示了用于心血管疾病早期临床诊断的即时检测的潜力。图形摘要 用血红素/G-四链体脱氧核糖核酸功能化的银纳米粒子 (AgNP) 的示意图，用于在玻璃芯片阵列上对心肌肌钙蛋白 T (cTnT) 进行高灵敏度化学发光 (CL) 免疫测定。用血清样品获得的结果与参考值一致。基于 AgNP 的示踪标签以及免疫测定方法显示了用于心血管疾病早期临床诊断的即时检测的潜力。图形摘要 用血红素/G-四链体脱氧核糖核酸功能化的银纳米粒子 (AgNP) 的示意图，用于在玻璃芯片阵列上对心肌肌钙蛋白 T (cTnT) 进行高灵敏度化学发光 (CL) 免疫测定。