The key factor in skeletal muscle tissue engineering is regeneration of the functional skeletal muscles. Materials that could promote the myoblast proliferation and myogenic differentiation are promising candidates in skeletal muscle tissue engineering. Herein, we developed an elastic conductive poly(ethylene glycol)-co-poly(glycerol sebacate) (PEGS) grafted aniline pentamer (AP) copolymer that could promote the formation of myotubes by differentiating the C2C12 myoblast cells. The results of hydration behavior and water contact angle suggested that by adjusting the poly(ethylene glycol) (PEG) and AP content, this film showed a proper surface hydrophilicity for cell attachment. Additionally, these films showed tunable conductivity and mechanical properties that can be altered by changing the AP content. The maximum conductivity of the films was 1.84 × 10^–4 S/cm and the Young’s modulus of these films ranged from 14.58 ± 1.35 MPa to 24.62 ± 0.61 MPa. Our findings indicate that the PEGS-AP films promote the proliferation and myogenic differentiation of C2C12 cells, suggesting that they are promising biomaterials for skeletal muscle tissue engineering.

中文翻译：

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生物相容性弹性导电膜显着增强了成肌细胞的骨骼肌再生的成肌分化。

骨骼肌组织工程的关键因素是功能性骨骼肌的再生。可以促进成肌细胞增殖和成肌分化的材料是骨骼肌组织工程中有希望的候选者。在本文中，我们开发了一个弹性导电聚（乙二醇） -共-聚癸二酸甘油酯（PEGS）接枝的苯胺五聚体（AP）共聚物，可通过分化C2C12成肌细胞来促进肌管的形成。水合行为和水接触角的结果表明，通过调节聚乙二醇（PEG）和AP的含量，该膜显示出适合细胞附着的表面亲水性。另外，这些薄膜显示出可调节的电导率和机械性能，可以通过改变AP含量来改变。薄膜的最大电导率为1.84×10 ^–4这些膜的S / cm和杨氏模量为14.58±1.35MPa至24.62±0.61MPa。我们的发现表明，PEGS-AP膜可促进C2C12细胞的增殖和成肌分化，表明它们是用于骨骼肌组织工程的有前途的生物材料。