Dicitrinone D is a novel polyketide isolated from a marine-derived fungus Penicillium citrinum. In this study, we described the isolation, structure elucidation and antitumor mechanism of this compound. Cell proliferation assay showed that dicitrinone D was safe for normal cells, but effectively inhibited the growth of multiple tumor cells, especially SPC-A1 cells. Cell cycle and immunofluorescence analysis suggested that dicitrinone D had cellular effects similar to other microtubule-targeting agents. Polymerization assay, electron microscopy and time-lapsed video microscopy assay demonstrated dicitrinone D was a microtubule-destabilizing agent. Finally, the docking analysis predicted that dicitrinone D may bind to vinblastine binding site on tubulin.

Dicitrinone D是一种新的聚酮化合物，它是从海洋衍生的柠檬青霉菌中分离得到的。在这项研究中，我们描述了该化合物的分离，结构阐明和抗肿瘤机理。细胞增殖试验表明，双西西醌D对正常细胞是安全的，但有效抑制多种肿瘤细胞，特别是SPC-A1细胞的生长。细胞周期和免疫荧光分析表明，双西替利酮D具有类似于其他微管靶向剂的细胞作用。聚合测定，电子显微镜和延时视频显微镜测定表明，地西西醌D是微管去稳定剂。最后，对接分析预测，双西替尼酮D可能与微管蛋白上的长春碱结合位点结合。