In the present work, we investigated the synchronization of electrical activity in cultured cardiac cells of different origin put in direct contact. In the first set of experiments synchronization was studied in the primary culture cells of neonatal rats taken at different developmental ages, and in the second – in the neonatal rat cardiomyocytes and HL-1 cells. The electrical excitation of cells was recorded using the calcium transient marker Fluor-4. In the confluent cell layers created with the aid of a specially devised mask, the excitation waves and their propagation between areas occupied by cells of different origin were observed. On the level of individual cells, their contact and synchronization was monitored with the aid of scanning fluorescence microscopy. It was found that populations of cultured cells of different origin are able to synchronize, suggesting the formation of electrical coupling between them. The results obtained may be considered as a proof of concept that implanted alien grafted cells are able to create electrical coupling with the host cardiac tissue.

中文翻译：

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不同来源的可激发心脏培养物的同步

在本工作中，我们研究了直接接触的不同来源的培养心肌细胞中电活动的同步性。在第一组实验中，在不同发育年龄的新生大鼠的原代培养细胞中研究了同步性，在第二组实验中，研究了新生大鼠的心肌细胞和HL-1细胞中的同步性。使用钙瞬变标记Fluor-4记录细胞的电激发。在借助于专门设计的掩模形成的汇合细胞层中，观察到激发波及其在不同来源细胞所占区域之间的传播。在单个细胞的水平上，借助于扫描荧光显微镜监测它们的接触和同步。发现不同来源的培养细胞群体能够同步，这表明它们之间形成了电耦合。获得的结果可以被认为是植入的外来移植细胞能够与宿主心脏组织产生电耦合的概念证明。