Fabrication and characterization of a surface plasmon resonance based fiber optic xanthine sensor using entrapment of xanthine oxidase (XO) enzyme in several nanostructures of tantalum (v) oxide (Ta₂O₅) have been reported. Chemical route was adopted for synthesizing Ta₂O₅ nanoparticles, nanorods, nanotubes and nanowires while Ta₂O₅ nanofibers were prepared by electrospinning technique. The synthesized Ta₂O₅ nanostructures were characterized by photoluminescence, scanning electron microscopy, UV–Visible spectra and X-ray diffraction pattern. The probes were fabricated by coating an unclad core of the fiber with silver layer followed by the deposition of XO entrapped Ta₂O₅ nanostructures. The crux of sensing mechanism relies on the modification of dielectric function of sensing layer upon exposure to xanthine solution of diverse concentrations, reflected in terms of shift in resonance wavelength. The sensing probe coated with XO entrapped Ta₂O₅ nanofibers has been turned out to possess maximum sensitivity amongst the synthesized nanostructures. The probe was optimized in terms of pH of the sample and the concentration of XO entrapped in Ta₂O₅ nanofibers. The optimized sensing probe possesses a remarkably good sensitivity of 26.2 nm/µM in addition to linear range from 0 to 3 µM with an invincible LOD value of 0.0127 µM together with a response time of 1 min. Furthermore, probe selectivity with real sample analysis ensure the usage of the sensor for practical scenario. The results reported open a novel perspective towards a sensitive, rapid, reliable and selective detection of xanthine.

已经报道了使用在氧化钽（v）的几个纳米结构（Ta ₂ O ₅）中截留黄嘌呤氧化酶（XO）酶来制造和表征基于表面等离子体共振的光纤黄嘌呤传感器。采用化学路线合成Ta ₂ O ₅纳米粒子，纳米棒，纳米管和纳米线，同时通过静电纺丝技术制备Ta ₂ O ₅纳米纤维。合成的Ta ₂ O ₅纳米结构的特征在于光致发光，扫描电子显微镜，紫外可见光谱和X射线衍射图。通过用银层涂覆纤维的未包层芯，然后沉积XO包裹的Ta ₂ O ₅纳米结构来制造探针。传感机制的症结在于，当暴露于不同浓度的黄嘌呤溶液时，传感层的介电功能会发生变化，这反映了共振波长的变化。XO包裹的Ta ₂ O ₅包覆的传感探头事实证明，纳米纤维在合成的纳米结构中具有最大的敏感性。根据样品的pH值和Ta ₂ O ₅纳米纤维中截留的XO浓度优化了探针。经过优化的传感探头，除了线性范围为0至3 µM，无敌LOD值为0.0127 µM，响应时间为1分钟之外，还具有26.2 nm / µM的出色灵敏度。此外，具有真实样品分析功能的探针选择性可确保在实际情况下使用传感器。结果报告开辟了一种灵敏，快速，可靠和选择性检测黄嘌呤的崭新视角。