As an outstanding candidate of molecular logic computing, DNA logic computing has gained extensive advancements across diverse research areas. Nevertheless, current DNA logic gates with various functions are fragmentary nominated and always constructed separately due to their different operating-principles. Tedious/obligatory gates’ redesign/reoperation resulted in longer time, higher costs and lower computing efficiency. Herein, we, for the first time, propose the concept of “Contrary Logic Pairs” to systematically classify DNA gates with opposite functions into “positive^negative” gates (CLP = Pos^Neg). By utilizing two fluorescent substrates (Amplex Red, Scopoletin) of G-quadruplex DNAzyme as label-free signal-reporters, based solely on DNA hybridization, we fabricated the first intelligent universal system that yields double results with half the effort for engineering a DNA CLPs library and various DNA combinatorial logic circuits. Differing from previous DNA logic systems, as for the non-interference between two substrates, “Pos^Neg” gates of each DNA CLPs in this system were operated via the same DNA reaction at one time, without gates’ redesign/reoperation. With the modulation of DNA reactions, a DNA CLPs library was fabricated. Moreover, through switching the selective/parallel operating-mode, “Pos^Neg” gates of each CLP could not only be alternatively constructed, but also be integrated into DNA combinatorial logic circuits (Pos/p/Neg). All the strategies largely simplified the operation and reduced the time/costs of current DNA gates’ construction by at least 1/2, accompanied with significantly improved computing efficiency. Furthermore, a DNA voter with “One-vote Deny” function that executed by multiple equal deniers was realized.

中文翻译：

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一个智能的通用系统只需花费一半的精力即可设计出DNA“矛盾逻辑对”文库和各种DNA组合逻辑电路，从而获得双倍的结果

作为分子逻辑计算的杰出候选人，DNA逻辑计算在各个研究领域都取得了长足的进步。然而，当前具有各种功能的DNA逻辑门是片段提名的，并且由于它们的工作原理不同，总是分开构造。繁琐/强制性的闸门的重新设计/重新操作导致更长的时间，更高的成本和更低的计算效率。在此，我们首次提出“相反逻辑对”的概念，将具有相反功能的DNA门系统地分类为“正^负”门（CLP = Pos ^ Neg）。通过仅基于DNA杂交，将G-四链体DNAzyme的两种荧光底物（Amplex Red，Scopoletin）用作无标记信号报告物，我们制造出了第一个智能通用系统，该系统用一半的工作量即可完成DNA CLPs库和各种DNA组合逻辑电路的工程设计，从而产生双倍的结果。与先前的DNA逻辑系统不同，关于两个底物之间的不干扰，该系统中每个DNA CLP的“ Pos ^ Neg”门均被操作一次通过相同的DNA反应进行反应，无需盖茨重新设计/重新操作。通过调节DNA反应，制备了DNA CLPs文库。此外，通过切换选择性/并行操作模式，每个CLP的“ Pos ^ Neg”门不仅可以交替构建，而且可以集成到DNA组合逻辑电路（Pos / p / Neg）中。所有这些策略在很大程度上简化了操作，并将当前DNA门的构建时间/成本降低了至少1/2，同时显着提高了计算效率。此外，实现了具有“一票否决”功能的DNA投票器，该功能由多个相等的旦尼尔执行。