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Evaluating Nitrogen-Containing Biosynthetic Products Produced by Saltwater Culturing of Several California Littoral Zone Gram-Negative Bacteria
Journal of Natural Products ( IF 5.1 ) Pub Date : 2017-08-04 00:00:00 , DOI: 10.1021/acs.jnatprod.7b00302
Nicholas Lorig-Roach 1 , Patrick C Still 1 , David Coppage 1 , Jennifer E Compton 1 , Mitchell S Crews 1 , Gabriel Navarro 1 , Karen Tenney 1 , Phillip Crews 1
Affiliation  

The biosynthetic potential of marine-sediment-derived Gram-negative bacteria is poorly understood. Sampling of California near-shore marine environments afforded isolation of numerous Gram-negative bacteria in the Proteobacteria and Bacteriodetes phyla, which were grown in the laboratory to provide extracts whose metabolites were identified by comparative analyses of LC-mass spectrometry and MSn data. Overall, we developed an assemblage of seven bacterial strains grown in five different media types designed to coax out unique secondary metabolite production as a function of varying culture conditions. The changes in metabolite production patterns were tracked using the GNPS MS2 fragmentation pattern analysis tool. A variety of nitrogen-rich metabolites were visualized from the different strains grown in different media, and strikingly, all of the strains examined produced the same new, proton-atom-deficient compound, 1-methyl-4-methylthio-β-carboline (1), C13H12N2S. Scale-up liquid culture of Achromobacter spanius (order: Burkholderiales; class: Betaproteobacteria) provided material for the final structure elucidation. The methods successfully combined in this work should stimulate future studies of molecules from marine-derived Gram-negative bacteria.

中文翻译:

评估几种加州滨海区革兰氏阴性细菌盐水培养产生的含氮生物合成产品

人们对海洋沉积物来源的革兰氏阴性细菌的生物合成潜力知之甚少。对加州近岸海洋环境进行采样,分离出变形菌门和拟杆菌门中的大量革兰氏阴性细菌,这些细菌在实验室中生长,提供提取物,通过 LC 质谱和 MS n 数据的比较分析鉴定其代谢。总体而言,我们开发了在五种不同培养基类型中生长的七种细菌菌株的组合,旨在根据不同的培养条件诱导独特的次级代谢产物的产生。使用 GNPS MS 2碎片模式分析工具跟踪代谢物生产模式的变化。在不同培养基中生长的不同菌株中观察到了多种富氮代谢物,令人惊讶的是,所有检查的菌株都产生了相同的新的质子原子缺陷化合物,1-甲基-4-甲硫基-β-咔啉(1-methyl-4-methylthio-β-carboline)。1 ),C 13 H 12 N 2 S。西班牙无色杆菌(目:伯克霍尔德菌目;纲:β-变形菌纲)的放大液体培养物为最终结构阐明提供了材料。这项工作中成功结合的方法应该会刺激未来对海洋来源的革兰氏阴性细菌分子的研究。
更新日期:2017-08-04
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