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Chaperone-Polymer-Assisted, Photodriven DNA Strand Displacement
ChemBioChem ( IF 3.2 ) Pub Date : 2017-08-01 06:10:35 , DOI: 10.1002/cbic.201700394
Bohao Cheng 1 , Hiromu Kashida 1 , Naohiko Shimada 2 , Atsushi Maruyama 2 , Hiroyuki Asanuma 1
Affiliation  

The cover picture shows how a reversible DNA-strand-displacement system is photoregulated, and assisted by a chaperon polymer, PLL-g-Dex. With visible light irradiation, a strand incorporated with azobenzene moieties (in the trans form) can hybridize its complementary strand and release another without any toehold domain; with UV irradiation, these azobenzenes (in the cis form) devastatingly destabilize the duplex, resulting in reversed strand displacement. This clean and reversible light-driven process subverts these defects in classical toehold strand displacement and is expected to bring in a new tool in nucleic acid isothermal amplification and DNA nanotechnology. More information can be found in the communication by A. Maruyama, H. Asanuma, et al. (DOI: 10.1002/cbic.201700202).

中文翻译:

伴侣聚合物辅助的光驱动DNA链置换

封面图片显示了如何对可逆DNA链置换系统进行光调节,并在分子伴侣聚合物PLL- g- Dex的辅助下进行。在可见光照射下,掺有偶氮苯部分的链(以反式形式)可以使其互补链杂交并释放出另一条没有任何鞋头域的结构。在紫外线照射下,这些偶氮苯(顺式形式)破坏了双链体的稳定性,导致反向链置换。这种清洁且可逆的光驱动过程颠覆了传统脚趾链移位中的这些缺陷,有望为核酸等温扩增和DNA纳米技术带来新的工具。可以在A. Maruyama,H。Asanuma等人的来文中找到更多信息。(DOI:10.1002 / cbic.201700202)。
更新日期:2017-08-02
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