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In Situ Ligation of High‐ and Low‐Affinity Ligands to Cell Surface Receptors Enables Highly Selective Recognition
Advanced Science ( IF 15.1 ) Pub Date : 2017-07-28 , DOI: 10.1002/advs.201700147
Misako Taichi 1 , Shogo Nomura 1 , Ikuhiko Nakase 2 , Rie Imamaki 3 , Yasuhiko Kizuka 3 , Fumi Ota 3 , Naoshi Dohmae 4 , Shinobu Kitazume 3 , Naoyuki Taniguchi 3 , Katsunori Tanaka 1, 5, 6
Affiliation  

This paper reports an entirely unexplored concept of simultaneously recognizing two receptors using high‐ and low‐affinity ligands through ligating them in situ on the target cell surface. This de novo approach is inspired by the pretargeting strategy frequently applied in molecular imaging, and has now evolved as the basis of a new paradigm for visualizing target cells with a high imaging contrast. A distinct advantage of using a labeled low‐affinity ligand such as glycan is that the excess labeled ligand can be washed away from the cells, whereas the ligand bound to the cell, even at the milli molar affinity level, can be anchored by a bioorthogonal reaction with a pretargeted high‐affinity ligand on the surface. Consequently, nonspecific background is minimized, leading to improved imaging contrast. Importantly, despite previously unexplored for molecular imaging, a notoriously weak glycan/lectin interaction can now be utilized as a highly selective ligand to the targets.

中文翻译:

高亲和力和低亲和力分子与细胞表面受体的原位连接可实现高度选择性的识别

本文报道了一个完全尚未探索的概念,即通过将高亲和力和低亲和力的配体通过在目标细胞表面上原位连接来同时识别两个受体。这种从头开始的方法受到分子成像中经常应用的预靶向策略的启发,现在已经发展成为一种新的范例,可以可视化具有高成像对比度的靶细胞。使用标记的低亲和力配体(例如聚糖)的显着优势是可以将多余的标记配体从细胞上冲走,而结合至细胞的配体(即使在毫摩尔摩尔亲和力水平)也可以通过生物正交锚定与表面上预​​定的高亲和力配体发生反应。因此,非特定背景被最小化,从而改善了成像对比度。重要的,
更新日期:2017-07-28
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