An actinomycete strain, designated MM04-1133^T, was isolated from an anthill soil sample collected in Bagan, Myanmar. To establish the taxonomic status of this strain, the isolate was subjected to a polyphasic approach. Strain MM04-1133^T was Gram-staining positive, aerobic, motile and formed long and narrow sporangia directly above the surface of the substrate mycelium. Whole-cell hydrolysates of the strain contained 3-OH-diaminopimelic acid, arabinose, glucose, galactose, mannose, rhamnose and xylose. The predominant menaquinones were MK-10(H₆) and MK-10(H₈). The major cellular fatty acids were iso-C_16:0 and anteiso-C_17:0. The diagnostic phospholipid was phosphatidylethanolamine. The G+C content of the DNA was 69.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain MM04-1133^T clustered within the genus Virgisporangium, with the sequence exhibiting highest similarity (98.5% identity) with Virgisporangium ochraceum NBRC 16418^T. The strain grew in the presence of 0-1% (w/v) NaCl, at pH 5-8 and at 20-40 °C, with optimal growth at 30-37 °C. Based on phylogenetic analysis and chemotaxonomic and phenotypic data, we propose classifying this isolate as a novel species of the genus Virgisporangium, to be designated as Virgisporangium myanmarense sp. nov. The type strain is MM04-1133^T (=NBRC 112733^T=VTCC 910008^T).

中文翻译：

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Virgisporangium myanmarense sp。nov。，一种从缅甸蚁丘土壤中分离出来的新型运动放线菌。

的放线菌菌株，命名MM04-1133 ^Ť，从在蒲甘，缅甸收集蚁土壤样品中分离出来。为了确定该菌株的分类学状态，对分离株进行了多相分析。菌株MM04-1133 ^T为革兰氏染色阳性，有氧，运动型，在底质菌丝体表面正上方形成长而狭窄的孢子囊。该菌株的全细胞水解产物包含3-OH-二氨基庚二酸，阿拉伯糖，葡萄糖，半乳糖，甘露糖，鼠李糖和木糖。主要的甲萘醌为MK-10（H ₆）和MK-10（H ₈）。主要的细胞脂肪酸是iso-C _16：0和anteiso-C _17：0。诊断性磷脂是磷脂酰乙醇胺。DNA的G + C含量为69.1mol％。根据16S rRNA基因序列的系统进化分析表明，菌株MM04-1133 ^T聚集在Virgisporangium属中，与该序列具有最高的相似性（98.5％的同一性），与Virgisporangium ochraceum NBRC 16418 ^T相似。该菌株在0-1％（w / v）NaCl的存在下，pH 5-8和20-40°C下生长，最佳生长在30-37°C下。基于系统发育分析和化学分类学和表型数据，我们建议将此分离物归类为Virgisporangium属的一个新物种，被命名为Virgisporangium myanmarense sp。十一月 类型应变为MM04-1133 ^T（= NBRC 112733 ^T = VTCC 910008 ^T）。