The study of cancer growth mechanisms and the determination of the efficacy of experimental therapeutics are usually performed in two-dimensional (2D) cell culture models. However, these models are incapable of mimicking complex interactions between cancer cells and the environment. With the advent of microfluidic technologies, the combination of multiple cell cultures with mechanical and biochemical stimuli has enabled a better recapitulation of the three-dimensional (3D) tumor environment using minute amounts of reagents. These models can also be used to study drug transport, hypoxia, and interstitial pressure within the tumor. In this review, we highlight the applications of microfluidic-based models in anticancer drug studies and provide a perspective on the future of the clinical applications of microfluidic systems for anticancer drug development.

通常在二维（2D）细胞培养模型中进行癌症生长机制的研究和实验治疗剂功效的确定。但是，这些模型无法模拟癌细胞与环境之间的复杂相互作用。随着微流体技术的出现，将多种细胞培养物与机械和生化刺激物结合使用，可以使用少量试剂更好地再现三维（3D）肿瘤环境。这些模型还可以用于研究肿瘤内的药物转运，缺氧和组织间压力。在这篇评论中，