Baranovskiy et al. and Pellegrini argue that, based on structural data, the path for charge transfer through the [4Fe4S] domain of primase is not feasible. Our manuscript presents electrochemical data directly showing charge transport through DNA to the [4Fe4S] cluster of a primase p58C construct and a reversible switch in the DNA-bound signal with oxidation/reduction, which is inhibited by mutation of three tyrosine residues. Although the dispositions of tyrosines differ in different constructs, all are within range for microsecond electron transfer.

中文翻译：

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对“[4Fe4S] 人类 DNA 引物酶簇作为使用 DNA 电荷传输的氧化还原开关”的评论的回应

巴拉诺夫斯基等人。和 Pellegrini 认为，根据结构数据，电荷转移通过 primase 的 [4Fe4S] 域的路径是不可行的。我们的手稿提供了电化学数据，直接显示电荷通过 DNA 传输到引物酶 p58C 构建体的 [4Fe4S] 簇，以及 DNA 结合信号的可逆转换与氧化/还原，这受到三个酪氨酸残基突变的抑制。尽管酪氨酸的配置在不同的结构中有所不同，但都在微秒电子转移的范围内。