Coacervates have been widely studied as model compartments in protocell research. Complex coacervates composed of disordered proteins and RNA have also been shown to play an important role in cellular processes. Herein, we report on a microfluidic strategy for constructing monodisperse coacervate droplets encapsulated within uniform unilamellar liposomes. These structures represent a bottom-up approach to hierarchically structured protocells, as demonstrated by storage and release of DNA from the encapsulated coacervates as well as localized transcription.

中文翻译：

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脂质体中单凝聚凝聚细胞器的微流体形成。

在原始细胞研究中，凝聚层已被广泛研究为模型区室。由杂乱的蛋白质和RNA组成的复杂凝聚层也已被证明在细胞过程中起着重要作用。在本文中，我们报道了一种微流体策略，用于构建封装在均匀单层脂质体内的单分散凝聚层液滴。这些结构代表了一种从下而上的方法，用于分层结构化的原始细胞，如通过DNA从封装的凝聚层中的存储和释放以及局部转录所证明的那样。