The amplification- and enzyme-free quantification of DNA at ultralow concentrations, on the order of 10–1000 targets, is highly beneficial but extremely challenging. To address this challenge, true detection signals must be reliably discriminated from false or noise signals. Herein, we describe the development of associating and dissociating nanodimer analysis (ADNA) as a method that enables a maximum number of detection signals to be collected from true target-binding events while keeping nonspecific signals at a minimum level. In the ADNA assay for ultralow target concentrations, Au nanoprobes on a lipid micropattern were monitored and analyzed in situ, and newly defined dissociating dimers, which are eventually decoupled into monomers again, were incorporated into the detection results. Tens to thousands of DNA copies can be reliably quantified with excellent single-base-mismatch differentiation capability by this non-enzymatic, amplification-free ADNA method.

中文翻译：

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缔合和解离纳米二聚体分析定量超少量DNA

超低浓度DNA的无扩增和无酶定量（约10–1000个靶标）是非常有益的，但极具挑战性。为了应对这一挑战，必须可靠地将真实的检测信号与错误或噪声信号区分开。在本文中，我们描述了缔合和解离纳米二聚体分析（ADNA）作为一种方法的发展，该方法能够从真实的靶结合事件中收集最大数量的检测信号，同时将非特异性信号保持在最低水平。在针对超低目标浓度的ADNA分析中，对脂质微模式上的Au纳米探针进行了监测和原位分析，并将新定义的解离二聚体（最终再次解离为单体）纳入了检测结果。