The presence of a protein corona on various synthetic nanomaterials has been shown to strongly influence how they interact with cells. However, it is unclear if the protein corona also exists on protein particles, and if so, its role in particle–cell interactions. In this study, pure human serum albumin (HSA) particles were fabricated via mesoporous silica particle templating. Our data reveal that various serum proteins adsorbed on the particles, when exposed to human blood plasma, forming a corona. In human umbilical vein endothelial cells (HUVECs), the corona was shown to decrease particle binding to the cell membrane, increase the residence time of particles in early endosomes, and reduce the amount of internalized particles within the first hours of exposure to particles. These findings reveal important information regarding the mechanisms used by vascular endothelial cells to internalize protein-based particulate materials exposed to blood plasma. The ability to control the cellular recognition of these organic particles is expected to aid the advancement of HSA-based materials for intravenous drug delivery.

中文翻译：

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人血浆蛋白电晕在纳米孔人血清白蛋白颗粒与内皮细胞之间的细胞相互作用中的作用。

各种合成纳米材料上存在蛋白质电晕，已显示出强烈影响它们与细胞相互作用的方式。然而，目前尚不清楚蛋白质电晕是否也存在于蛋白质颗粒上，如果存在，还不清楚其在颗粒-细胞相互作用中的作用。在这项研究中，通过介孔二氧化硅颗粒模板制备了纯净的人血清白蛋白（HSA）颗粒。我们的数据显示，当暴露于人血浆中时，各种血清蛋白会吸附在颗粒上，形成电晕。在人脐静脉内皮细胞（HUVEC）中，电晕显示减少了颗粒与细胞膜的结合，增加了颗粒在早期内体中的停留时间，并减少了暴露于颗粒中的最初几小时内的内在化颗粒数量。这些发现揭示了有关血管内皮细胞用于内化暴露于血浆的基于蛋白质的颗粒物质的机制的重要信息。预期控制这些有机颗粒的细胞识别能力将有助于基于HSA的材料用于静脉内药物输送的发展。