MALDI MS with its long history in clinical analysis, is a vital tool, well known for its versatility, rapidity and implementation ease. In tuberculosis research, MALDI has outstanding contributions on the pathogen detection aspect as well as disease diagnosis prospect. This article reviews the existing methods and achievements made employing MALDI MS in tuberculosis research. The challenges confronting and limiting MALDI MS based tuberculosis analysis are presented. An interesting yet intriguing lacuna is that almost none of the state-of-art MALDI MS variants, such as DIOS, NIMS, NALDI, NAPA have been implicated into MALDI based tuberculosis research. These nano platforms holding fabulous claims for pushing the limits of detection and avoiding matrix interferences, enhanced resolution, being kept away from this research arena is disturbing and is called to notice through this review.

中文翻译：

---

用于结核病诊断的临床MALDI质谱法：推测方法蓝图并考虑即兴创作的义务

MALDI MS具有悠久的临床分析历史，是一种至关重要的工具，以其多功能性，快速性和易于实施而著称。在结核病研究中，MALDI在病原体检测方面以及疾病诊断前景方面都做出了杰出贡献。本文回顾了使用MALDI MS进行结核病研究的现有方法和取得的成就。介绍了基于MALDI MS的结核病分析面临的挑战和局限性。一个有趣但引人入胜的缺陷是，几乎没有最新的MALDI MS变体（例如DIOS，NIMS，NALDI，NAPA）参与基于MALDI的结核病研究。这些纳米平台拥有非凡的主张，可以推动检测极限并避免基质干扰，提高分离度，