For the 1.7 million patients per year in the US who receive a new cancer diagnosis, treatment decisions are largely based on histopathological specimen examinations. Unfortunately, the gold standard of slide-based microscopic pathology suffers from high inter-observer variability and limited prognostic value due to sampling limitations and the inability to visualize tissue structures and molecular targets in their native 3D context. Here, we show that an open-top light-sheet microscope optimized for non-destructive slide-free pathology of clinical specimens enables the rapid imaging of intact tissues at high resolution over large 2D and 3D fields of view, with the same level of detail as traditional pathology. We demonstrate the utility of this technology for various applications: wide-area surface microscopy to triage surgical specimens (with ~200 μm surface irregularities), rapid intraoperative assessment of tumour-margin surfaces (12.5 s cm⁻²), and volumetric assessment of optically cleared core-needle biopsies (1 mm in diameter, 2 cm in length). Light-sheet microscopy can be a versatile tool for both rapid surface microscopy and deep volumetric microscopy of human specimens.

在美国，每年有170万人接受新的癌症诊断，因此，治疗决定主要取决于组织病理学标本检查。不幸的是，基于幻灯片的微观病理学的金标准由于样本的局限性以及无法在其原始3D环境下可视化组织结构和分子靶标而遭受高观察者间差异和有限的预后价值的困扰。在这里，我们展示了一种针对临床标本的非破坏性无滑动病理而优化的敞口式光片显微镜，能够以相同的详细程度在较大的2D和3D视野内以高分辨率对完整的组织进行快速成像。作为传统病理。我们演示了该技术在各种应用中的实用性：^-2），以及光学清除的芯针活检的体积评估（直径1毫米，长度2厘米）。光片显微镜可以是用于人类标本的快速表面显微镜和深体积显微镜的多功能工具。