当前位置: X-MOL 学术Annu. Rev. Biochem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Mechanics and Single-Molecule Interrogation of DNA Recombination
Annual Review of Biochemistry ( IF 16.6 ) Pub Date : 2016-06-13 00:00:00 , DOI: 10.1146/annurev-biochem-060614-034352
Jason C Bell 1 , Stephen C Kowalczykowski 1
Affiliation  

The repair of DNA by homologous recombination is an essential, efficient, and high-fidelity process that mends DNA lesions formed during cellular metabolism; these lesions include double-stranded DNA breaks, daughter-strand gaps, and DNA cross-links. Genetic defects in the homologous recombination pathway undermine genomic integrity and cause the accumulation of gross chromosomal abnormalities—including rearrangements, deletions, and aneuploidy—that contribute to cancer formation. Recombination proceeds through the formation of joint DNA molecules—homologously paired but metastable DNA intermediates that are processed by several alternative subpathways—making recombination a versatile and robust mechanism to repair damaged chromosomes. Modern biophysical methods make it possible to visualize, probe, and manipulate the individual molecules participating in the intermediate steps of recombination, revealing new details about the mechanics of genetic recombination. We review and discuss the individual stages of homologous recombination, focusing on common pathways in bacteria, yeast, and humans, and place particular emphasis on the molecular mechanisms illuminated by single-molecule methods.

中文翻译:


DNA 重组的力学和单分子审讯

通过同源重组修复 DNA 是修复细胞代谢过程中形成的 DNA 损伤的重要、高效和高保真过程;这些损伤包括双链 DNA 断裂、子链缺口和 DNA 交联。同源重组途径中的遗传缺陷会破坏基因组完整性,并导致严重的染色体异常(包括重排、缺失和非整倍体)累积,从而导致癌症形成。重组通过联合 DNA 分子的形成进行 - 同源配对但亚稳态的 DNA 中间体,由几种替代子途径处理 - 使重组成为修复受损染色体的通用且强大的机制。现代生物物理方法使可视化、探测、并操纵参与重组中间步骤的单个分子,揭示有关基因重组机制的新细节。我们回顾和讨论同源重组的各个阶段,重点关注细菌、酵母和人类的共同途径,并特别强调单分子方法阐明的分子机制。

更新日期:2016-06-13
down
wechat
bug