A new enzyme catalyst consisting of pyrenecarboxaldehyde (PCA) and glucose oxidase (GOx) immobilized on polyethyleneimine (PEI) and a carbon nanotube supporter (CNT/PEI/[PCA/GOx]) is suggested, and the performance and stability of an enzymatic biofuel cell (EBC) using the new catalyst are evaluated. Using PCA, the amount of immobilized GOx increases (3.3 U mg⁻¹) and the electron transfer rate constant of the CNT/PEI/[PCA/GOx] is promoted (11.51 s⁻¹). Also, the catalyst induces excellent EBC performance (maximum power density (MPD) of 2.1 mW cm⁻²), long-lasting stability (maintenance of 93% of the initial MPD after 4 weeks) and superior catalytic activity (flavin adenine dinucleotide redox reaction rate of 0.62 mA cm⁻² and Michaelis–Menten constant of 0.99 mM). These characteristics are ascribed to effects of (i) electron collection due to hydrophobic interactions, (ii) electron transfer pathways due to π-conjugated bonds and (iii) enzyme stabilization due to π-hydrogen bonds that are newly induced by the PCA/GOx composite. The existence of such positive interactions is properly verified using X-ray photoelectron spectroscopy and enzyme activity measurements.

提出了一种新的酶催化剂，该酶催化剂由固定在聚乙烯亚胺（PEI）和碳纳米管载体（CNT / PEI / [PCA / GOx]）上的pyr甲醛（PCA）和葡萄糖氧化酶（GOx）组成，并且具有酶促生物燃料的性能和稳定性。使用新催化剂的电池（EBC）进行了评估。使用PCA，固定化的GOx的量增加（3.3U mg ^-1），并且促进了CNT / PEI / [PCA / GOx]的电子传递速率常数（11.51 s ^-1）。另外，该催化剂还具有出色的EBC性能（最大功率密度（MPD）为2.1 mW cm ^-2），持久的稳定性（4周后可维持初始MPD的93％）和优异的催化活性（黄素腺嘌呤二核苷酸氧化还原反应） 0.62 mA cm ^-2的速率和迈克尔尼斯-门腾常数0.99 m M）。这些特性归因于以下因素的影响：（i）由于疏水相互作用而产生的电子收集，（ii）由于π共轭键引起的电子转移途径和（iii）由于PCA / GOx新诱导的π-氢键引起的酶稳定作用合成的。使用X射线光电子能谱和酶活性测量可以正确地验证这种正相互作用的存在。