We describe a lanthanide biosensor that responds to CDK4 kinase activity in melanoma cell extracts through a significant and dose dependent increase in luminescence, thanks to sensitization of a DOTA[Tb³⁺] complex incorporated into a CDK4 substrate peptide by a unique tryptophan residue in an adjacent phosphoaminoacid binding moiety.

中文翻译：

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基于镧系元素的肽生物传感器，可监测CDK4 / cyclin D激酶的活性

我们描述了一种镧系元素生物传感器，它通过显着和剂量依赖性的发光增加来响应黑素瘤细胞提取物中的CDK4激酶活性，这归因于DOTA [Tb ³⁺ ]复合物通过独特的色氨酸残基掺入CDK4底物肽中的敏化作用。相邻的磷酸氨基酸结合部分。