Resolving the spatial distribution of the human proteome at a subcellular level can greatly increase our understanding of human biology and disease. Here we present a comprehensive image-based map of subcellular protein distribution, the Cell Atlas, built by integrating transcriptomics and antibody-based immunofluorescence microscopy with validation by mass spectrometry. Mapping the in situ localization of 12,003 human proteins at a single-cell level to 30 subcellular structures enabled the definition of the proteomes of 13 major organelles. Exploration of the proteomes revealed single-cell variations in abundance or spatial distribution and localization of about half of the proteins to multiple compartments. This subcellular map can be used to refine existing protein-protein interaction networks and provides an important resource to deconvolute the highly complex architecture of the human cell.

中文翻译：

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人类蛋白质组的亚细胞图谱。

在亚细胞水平上解决人类蛋白质组的空间分布可以大大增加我们对人类生物学和疾病的理解。在这里，我们展示了一个基于图像的综合亚细胞蛋白质分布图，即细胞图谱，它是通过整合转录组学和基于抗体的免疫荧光显微镜以及质谱验证而构建的。将 12,003 个人类蛋白质在单细胞水平上的原位定位映射到 30 个亚细胞结构，从而能够定义 13 个主要细胞器的蛋白质组。对蛋白质组的探索揭示了单细胞丰度或空间分布的变化，以及大约一半的蛋白质定位于多个隔室。