In this tutorial review we describe a holistic approach to the invention, development and optimisation of biotransformations utilising isolated enzymes. Increasing attention to applied biocatalysis is motivated by its numerous economic and environmental benefits. Biocatalysis engineering concerns the development of enzymatic systems as a whole, which entails engineering its different components: substrate engineering, medium engineering, protein (enzyme) engineering, biocatalyst (formulation) engineering, biocatalytic cascade engineering and reactor engineering.

中文翻译：

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生物催化工程：大局

在本教程的复习中，我们描述了利用分离的酶对生物转化进行发明，开发和优化的整体方法。大量的经济和环境利益促使人们越来越重视应用生物催化。生物催化工程涉及整个酶系统的发展，这涉及工程其不同的组成部分：底物工程，介质工程，蛋白质（酶）工程，生物催化剂（配方）工程，生物催化级联工程和反应器工程。