SUMMARY In the last 2 decades, renewed attention to neglected tropical diseases (NTDs) has spurred the development of antiparasitic agents, especially in light of emerging drug resistance. The need for new drugs has required in vitro screening methods using parasite culture. Furthermore, clinical laboratories sought to correlate in vitro susceptibility methods with treatment outcomes, most notably with malaria. Parasites with their various life cycles present greater complexity than bacteria, for which standardized susceptibility methods exist. This review catalogs the state-of-the-art methodologies used to evaluate the effects of drugs on key human parasites from the point of view of drug discovery as well as the need for laboratory methods that correlate with clinical outcomes.

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医学上重要的寄生虫的药敏试验[评论]

发明内容在过去的20年中，对被忽视的热带病（NTDs）的重新关注刺激了抗寄生虫药的发展，特别是考虑到新出现的耐药性。对新药的需求已要求使用寄生虫培养的体外筛选方法。此外，临床实验室试图将体外药敏方法与治疗效果相关联，最明显的是与疟疾相关联。具有各种生命周期的寄生虫比存在标准易感性方法的细菌具有更高的复杂性。这篇综述从药物发现的角度以及对与临床结果相关的实验室方法的需求出发，对用于评估药物对主要人体寄生虫的作用的最新方法进行了分类。