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Multiparametric Atomic Force Microscopy Imaging of Biomolecular and Cellular Systems
Accounts of Chemical Research ( IF 18.3 ) Pub Date : 2017-03-28 00:00:00 , DOI: 10.1021/acs.accounts.6b00638
David Alsteens 1 , Daniel J. Müller 2 , Yves F. Dufrêne 1, 3
Affiliation  

There is a need in biochemical research for new tools that can image and manipulate biomolecular and cellular systems at the nanoscale. During the past decades, there has been tremendous progress in developing atomic force microscopy (AFM) techniques to analyze biosystems, down to the single-molecule level. Force–distance (FD) curve-based AFM in particular has enabled researchers to map and quantify biophysical properties and biomolecular interactions on a wide variety of specimens. Despite its great potential, this AFM method has long been limited by its low spatial and temporal resolutions.

中文翻译:

生物分子和细胞系统的多参数原子力显微镜成像

在生物化学研究中,需要可以在纳米级成像和操纵生物分子和细胞系统的新工具。在过去的几十年中,在开发原子力显微镜(AFM)技术以分析生物系统到单分子水平方面取得了巨大的进步。特别是基于力距(FD)曲线的原子力显微镜,使研究人员能够绘制和量化各种标本的生物物理特性和生物分子相互作用。尽管有很大的潜力,但这种AFM方法长期以来一直受其低空间和时间分辨率的限制。
更新日期:2017-03-28
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