Dicarboxylic acids are commodity chemicals used in the production of plastics, polyesters, nylons, fragrances, and medications. Bio-based routes to dicarboxylic acids are gaining attention due to environmental concerns about petroleum-based production of these compounds. Some industrial applications require dicarboxylic acids with specific carbon chain lengths, including odd-carbon species. Biosynthetic pathways involving cytochrome P450-catalyzed oxidation of fatty acids in yeast and bacteria have been reported, but these systems produce almost exclusively even-carbon species. Here we report a novel pathway to odd-carbon dicarboxylic acids directly from glucose in Escherichia coli by employing an engineered pathway combining enzymes from biotin and fatty acid synthesis. Optimization of the pathway will lead to industrial strains for the production of valuable odd-carbon diacids.

中文翻译：

---

使用工程生物素-脂肪酸生物合成途径在大肠杆菌中生产奇数碳二羧酸

二羧酸是用于生产塑料、聚酯、尼龙、香料和药物的商品化学品。由于对基于石油的生产这些化合物的环境问题的关注，生产二羧酸的生物基路线越来越受到关注。一些工业应用需要具有特定碳链长度的二元羧酸，包括奇数碳物种。已经报道了涉及酵母和细菌中细胞色素 P450 催化氧化脂肪酸的生物合成途径，但这些系统几乎只产生偶碳物种。在这里，我们报告了一种通过将生物素和脂肪酸合成中的酶结合的工程途径，直接从大肠杆菌中的葡萄糖中获得奇数碳二羧酸的新途径。