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Genome-based characterization of a novel prophage of Vibrio parahaemolyticus, VPS05ph1, a novel member of Peduoviridae Virology (IF 3.7) Pub Date : 2024-04-10 Hailiang Wang, Guosi Xie, Jie Huang
is a globally important bacterium related to climate warming and health threat to human and marine animals. Yet, there is limited knowledge about its polylysogeny harboring multiple prophages and the genetic information. In this study, two prophages (VPS05ph1 and VPS05ph2) were identified in a isolate through genomic and transcriptional analyses. Both prophages were determined as HP1-like phages, located
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AMPK restricts HHV-6A replication by inhibiting glycolysis and mTOR signaling Virology (IF 3.7) Pub Date : 2024-04-09 Xiaodi Yang, Siyu Tian, Zhujiang Min, Emanuela Garbarino, Jingjing Ma, Junli Jia, Huamin Tang, Lingyun Li
AMP-activated protein kinase (AMPK) is a cellular energy sensor regulating metabolic homeostasis. In this study, we investigated the role of AMPK in response to human herpesvirus 6A (HHV-6A) infection. We show that HHV-6A infection significantly downregulates the active phosphorylated state of AMPK in infected T cells. Pharmacological activation of AMPK highly attenuated HHV-6A propagation. Mechanistically
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Assessment of the neutralizing antibody response in Omicron breakthrough cases in healthcare workers who received the homologous booster of Moderna mRNA-1273. Virology (IF 3.7) Pub Date : 2024-04-08 Constant Gillot, Marie Tré-Hadry, Roberto Cupaiolo, Laurent Blairon, Alain Wilmet, Ingrid Beukinga, Jean-Michel Dogné, Jonathan Douxfils, Julien Favresse
Vaccines against SARS-CoV-2 were developed during the pandemic including the BNT162b2 and the mRNA-1273. We evaluated the levels of binding antibodies against the receptor binding domain and the levels of NAbs in individuals who developed a breakthrough infection after having received three doses of mRNA-1273. A total of 51 participants were included. The breakthrough group was compared to a 1:1 matched-control
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Corrigendum to “Enhanced cancer cell killing by truncated E2F-1 used in combination with oncolytic adenovirus” [Virology 433 (2) (2012) 538–547] Virology (IF 3.7) Pub Date : 2024-04-08 Jorge G. Gomez-Gutierrez, Xia-Mei Rao, Heshan Sam Zhou, Kelly M. McMasters
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Eight-amino-acid sequence at the N-terminus of SARS-CoV-2 nsp1 is involved in stabilizing viral genome replication Virology (IF 3.7) Pub Date : 2024-04-08 Shiori Ueno, Sodbayasgalan Amarbayasgalan, Yoshiro Sugiura, Tatsuki Takahashi, Kenta Shimizu, Keisuke Nakagawa, Reika Kawabata-Iwakawa, Wataru Kamitani
Coronavirus disease 19 is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) enveloped virus with a single-stranded positive-sense ribonucleic acid (RNA) genome. The CoV non-structural protein (nsp) 1 is a multifunctional protein that undergoes translation shutoff, messenger RNA (mRNA) cleavage, and RNA binding. The C-terminal region is involved in translational shutoff and RNA
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TaWRKY50-TaSARK7 module-mediated cysteine-rich protein phosphorylation suppresses the programmed cell death response to Chinese wheat mosaic virus infection Virology (IF 3.7) Pub Date : 2024-04-05 Yunfei Guo, Yaoyao Jiang, Mila Wu, Aizhu Tu, Jingliang Yin, Jian Yang
WRKY transcription factors are widely involved in plant responses to biotic and abiotic stresses. However, there is currently a limited understanding of the regulation of viral infection by WRKY transcription factors in wheat (). The WRKY transcription factor in group IIb wheat exhibited a significant response to infection. is localized in the nucleus and is an activating transcription factor. Interestingly
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Opportunistic sampling of yellow canary (Crithagra flaviventris) has revealed a high genetic diversity of detected parvoviral sequences. Virology (IF 3.7) Pub Date : 2024-04-04 Yuhao Zhang, Saranika Talukder, Md Safiul Alam Bhuiyan, Lei He, Subir Sarker
Parvoviruses are known to be significant viral pathogens that infect a wide range of species globally. However, little is known about the parvoviruses circulating in Australian birds, including yellow canaries. Here, we present four parvoviral sequences including three novel parvoviruses detected from 10 yellow canaries (), named canary chaphamaparvovirus 1 and -2 (CaChPV1 and CaChPV2), canary dependoparvovirus
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Porcine respiratory coronavirus genome sequences; comparisons and relationships to transmissible gastroenteritis viruses Virology (IF 3.7) Pub Date : 2024-04-02 Amalie Ehlers Bedsted, Thomas Bruun Rasmussen, Laura D. Martinenghi, Anette Bøtner, Hans Nauwynck, Graham J. Belsham
Porcine respiratory coronavirus (PRCV) was initially detected in Europe, and later in the United States of America (US), in the 1980s. In this study we obtained and compared PRCV sequences from Europe and the US, and investigated how these are related to transmissible gastroenteritis virus (TGEV) sequences.
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Genetic insertion of mouse Myxovirus-resistance gene 1 increases innate resistance against both high and low pathogenic avian influenza virus by significantly decreasing replication in chicken DF1 cell line Virology (IF 3.7) Pub Date : 2024-03-31 Kelsey Briggs, Klaudia Chrzastek, Karen Segovia, Jongsuk Mo, Darrell R. Kapczynski
Avian influenza virus (AIV) is a constant threat to animal health with recent global outbreaks resulting in the death of hundreds of millions of birds with spillover into mammals. Myxovirus-resistance (Mx) proteins are key mediators of the antiviral response that block virus replication. Mouse (Mu) Mx (Mx1) is a strong antiviral protein that interacts with the viral nucleoprotein to inhibit polymerase
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Antiviral and synergistic effects of photo-energy with acyclovir on herpes simplex virus type 1 infection Virology (IF 3.7) Pub Date : 2024-03-31 Phil-Sun Oh, Yeon-Hee Han, SeokTae Lim, Berwin Singh Swami Vetha, Hwan-Jeong Jeong
This experimental study aimed to evaluate the antiviral and synergistic effects of photoenergy irradiation on human herpes simplex virus type I (HSV-1) infection. We assessed viral replication, plaque formation, and relevant viral gene expression to examine the antiviral and synergistic effects of blue light (BL) with acyclovir treatment. Our results showed that daily BL (10 J/cm) irradiation inhibited
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Furanocoumarins promote proteasomal degradation of viral HBx protein and down-regulate cccDNA transcription and replication of hepatitis B virus Virology (IF 3.7) Pub Date : 2024-03-24 Purnima Tyagi, Ankita Singh, Jitendra Kumar, Belal Ahmad, Aparna Bahuguna, Perumal Vivekanandan, Shiv Kumar Sarin, Vijay Kumar
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Vimentin inhibits peste des petits ruminants virus replication by interaction with nucleocapsid protein Virology (IF 3.7) Pub Date : 2024-03-24 Long Xu, Jingjing Ren, Lingxia Li, Mengyi Wang, Guoqiang Zhu, Haixue Zheng, Qiaoying Zeng, Youjun Shang, Dan Li
The Peste des petits ruminant virus (PPRV) is a member of the family and is classified into the genus Measles virus. PPRV predominantly infects small ruminants, leading to mortality rates of nearly 100%, which have caused significant economic losses in developing countries. Host proteins are important in virus replication, but the PPRV nucleocapsid (N) protein-host interacting partners for regulating
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Detection and genetic characterization of circulating canine parvovirus from stray dogs in Shanghai, China Virology (IF 3.7) Pub Date : 2024-03-23 Junyi Pu, Yan Zhang, Dengke Zhong, Qiusheng Chen
Canine parvovirus (CPV) is the main cause of viral diarrhea in dogs. CPV became a global disease in 1978 and was endemic all over the world. CPV-2 was the first strain to be identified, but with genetic mutations, new genotypes such as CPV-2a/2b/2c/new-2a/new-2b have emerged. In this study, 128 fecal samples of stray dogs suspected of CPV-2 infection were collected from January to March 2021 in Shanghai
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Diverse papillomaviruses identified from Antarctic fur seals, leopard seals and Weddell seals from the Antarctic Virology (IF 3.7) Pub Date : 2024-03-21 Melanie Regney, Simona Kraberger, Joy M. Custer, Adele E. Crane, Michelle R. Shero, Roxanne S. Beltran, Amy L. Kirkham, Koenraad Van Doorslaer, Anne C. Stone, Michael E. Goebel, Jennifer M. Burns, Arvind Varsani
Papillomaviruses (family ) are non-enveloped, circular, double-stranded DNA viruses known to infect squamous and mucosal epithelial cells. In the family there are 53 genera and 133 viral species whose members infect a variety of mammalian, avian, reptilian, and fish species. Within the Antarctic context, papillomaviruses (PVs) have been identified in Adélie penguins ( 2 PVs), Weddell seals ( 7 PVs)
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An isothermal nucleic acid amplification-based enzymatic recombinase amplification method for dual detection of porcine epidemic diarrhea virus and porcine rotavirus A Virology (IF 3.7) Pub Date : 2024-03-20 Qianwen Wu, Xing Liu, Jingyu Wang, Sijia Xu, Fanliang Zeng, Ling Chen, Guihong Zhang, Heng Wang
Viral diarrhea is the predominant digestive tract sickness in piglings, resulting in substantial profit losses in the porcine industry. Porcine rotavirus A (PoRVA) and porcine epidemic diarrhea virus (PEDV) are the main causes of grave gastroenteritis and massive dysentery, especially in piglets. PoRVA and PEDV have high transmissibility, exhibit similar clinical symptoms, and frequently co-occur.
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First detection of mycoviruses in Gnomoniopsis castaneae suggests a putative horizontal gene transfer event between negative-sense and double-strand RNA viruses Virology (IF 3.7) Pub Date : 2024-03-20 Franco Ferilli, Guglielmo Lione, Paolo Gonthier, Massimo Turina, Marco Forgia
is an ascomycetous fungus mainly known as a major pathogen of chestnut causing nut rots, although it is often found as an endophyte in chestnut tissues. To date, no virus has been reported as associated with to this fungus. Here, a collection of isolates from several European countries was screened to detect mycoviruses infecting the fungus: for the first time we report the identification and prevalence
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Introgression of OsAP47 by marker-assisted selection enhanced resistance against southern rice black-streaked dwarf virus disease Virology (IF 3.7) Pub Date : 2024-03-19 Feng Lin, Dedao Jing, Jiayuan Zhang, Yue Sun, Linlin Du, Chenyang Li, Ying Lan, Tong Zhou
Southern rice black-streaked dwarf virus disease (SRBSDVD) is the most destructive viral disease in rice. In order to breeding resistant cultivars, Insertion-Deletion (InDel) markers were developed linked to , the first isolated major resistance gene against SRBSDVD. Marker-assisted selection (MAS) was conducted to introduce this gene into the commercial variety. A rice line carrying homozygous resistance
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Distribution of human papillomavirus among Vietnamese women with cervical cancer and unusual genetic variability of HPV16 Virology (IF 3.7) Pub Date : 2024-03-16 Van Nam Le, Van Nguyen Bang Le, Xuan Son Hoang, Van Duyet Le
HPV16, with typical mutations that differ in geographical distribution and carcinogenic potency, has implications for cervical cancer screening, clinical diagnosis, and treatment. DNASTAR and MEGA were used to identify HPV16 variants and construct a phylogenetic tree. The most prevalent HPV genotypes were HPV16 (63.9%), HPV18 (26.7%), and other HPV (6.9%). HPV16 alterations were found in all E6, E7
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Molecular characterization and pathogenicity of a novel monopartite geminivirus infecting tobacco in China Virology (IF 3.7) Pub Date : 2024-03-15 Yuan Chen, Shiping Guo, Lianqiang Jiang, Fangfang Yan, Kaiqiang Hao, Zhiping Wang, Mengnan An, Zihao Xia, Fangfang Li, Xueping Zhou, Yuanhua Wu
The occurrence of geminiviruses causes significant economic losses in many economically important crops. In this study, a novel geminivirus isolated from tobacco in Sichuan province of China, named tomato leaf curl Chuxiong virus (TLCCxV), was characterized by small RNA-based deep sequencing. The full-length of TLCCxV genome was determined to be 2744 nucleotides (nt) encoding six open reading frames
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WSV2023 - The second meeting of the world society for virology: One health - One world - One virology Virology (IF 3.7) Pub Date : 2024-03-15 Ahmed S. Abdel-Moneim, Modra Murovska, Maria Söderlund-Venermo, Vikram N. Vakharia, William C. Wilson, Douglas P. Gladue, Matthew D. Moore, Covadonga Alonso, Sayed F. Abdelwahab, Marietjie Venter, Yashpal S. Malik, Shi Zhengli, Shailendra K. Saxena, Anupam Varma, Richard J. Kuhn
The Second International Conference of the World Society for Virology (WSV), hosted by Riga Stradiņš University, was held in Riga, Latvia, on June 15–17th, 2023. It prominently highlighted the recent advancements in different disciplines of virology. The conference had fourteen keynote speakers covering diverse topics, including emerging virus pseudotypes, Zika virus vaccine development, herpesvirus
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Increased expression of CD38 on endothelial cells in SARS-CoV-2 infection in cynomolgus macaques Virology (IF 3.7) Pub Date : 2024-03-14 Cong Thanh Nguyen, Misako Nakayama, Hirohito Ishigaki, Yoshinori Kitagawa, Akemi Kakino, Marumi Ohno, Masashi Shingai, Yasuhiko Suzuki, Tatsuya Sawamura, Hiroshi Kida, Yasushi Itoh
SARS-CoV-2 infection causes activation of endothelial cells (ECs), leading to dysmorphology and dysfunction. To study the pathogenesis of endotheliopathy, the activation of ECs in lungs of cynomolgus macaques after SARS-CoV-2 infection and changes in nicotinamide adenine dinucleotide (NAD) metabolism in ECs were investigated, with a focus on the CD38 molecule, which degrades NAD in inflammatory responses
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Modulation of autophagy affected tumorigenesis induced by the envelope glycoprotein of JSRV Virology (IF 3.7) Pub Date : 2024-03-13 Liang Zhang, Hui Yang, Xujie Duan, Huiping Li, Siriguleng Xu, Hui Chen, Jinlin Wang, Yu Wang, Shuying Liu
Ovine pulmonary adenocarcinoma (OPA), caused by the jaagsiekte sheep retrovirus (JSRV), is a chronic, progressive, and contagious lung tumor that seriously affects sheep production. It also represents a valuable animal model for several human lung adenocarcinomas. However, little is known about the role of autophagy in OPA tumorigenesis. Here, Western blotting combined with transmission electron microscopy
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Inhibition of PEDV viral entry upon blocking N-glycan elaboration Virology (IF 3.7) Pub Date : 2024-03-12 Yong Zhao, Tao Tang, Wenchang Zhao, Weiguang Fu, Tao Li
Porcine Epidemic Diarrhea Virus (PEDV) poses a significant threat to the global swine industry, demanding a thorough understanding of its cellular invasion mechanism for effective interventions. This study meticulously investigates the impact of O- and -linked glycans on PEDV proteins and host cell interaction, shedding light on their influence on the virus's invasion process.
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Human parainfluenza virus type 2 V protein inhibits mitochondrial apoptosis pathway through two ways Virology (IF 3.7) Pub Date : 2024-03-11 Naoki Saka, Machiko Nishio, Keisuke Ohta
Paramyxoviruses are reported to block apoptosis for their replication, but the mechanisms remain unclear. Furthermore, regulation of mitochondrial apoptosis by paramyxoviruses has been hardly reported. We investigated whether and how human parainfluenza virus type 2 (hPIV-2) counteracts apoptosis. Infection of recombinant hPIV-2 carrying mutated V protein showed higher caspase 3/7 activity and higher
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Natural hyperoside extracted from hawthorn exhibits antiviral activity against porcine epidemic diarrhea virus in vitro and in vivo Virology (IF 3.7) Pub Date : 2024-03-11 Jun Wang, Haibo Sun, Mingjun Su, Zijian Li, Lu Li, Feiyu Zhao, Yongchen Zhang, Wenfei Bai, Shiping Yu, Xu Yang, Shanshan Qi, Dan Yang, Donghua Guo, Chunqiu Li, Qinghe Zhu, Xiaoxu Xing, Dongbo Sun
Porcine epidemic diarrhea virus (PEDV) causes severe diarrhea and death in piglets, resulting in significant economic losses for the pork industry. There is an urgent need for new treatment strategies. Here, we focused on optimizing the process of purifying natural hyperoside (nHYP) from hawthorn and evaluating its effectiveness against PEDV both and . Our findings demonstrated that nHYP with a purity
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The investigation of the dynamics of changes in neutralizing antibody titers against type 5 adenovirus in the context of vaccination against a new coronavirus infection Virology (IF 3.7) Pub Date : 2024-03-08 I.V. Amosova, T.A. Timoshicheva, R.A. Kadyrova, Y.A. Zabrodskaya, V.S. Vakin, M.P. Grudinin, V.V. Dzytseva, M.S. Khmelevsky, D.A. Lioznov
This research focuses on analyzing the dynamics of neutralizing antibody (nAbs) titers against type 5 adenovirus (Ad5) in the adult population of Russia following vaccination against the novel coronavirus infection with recombinant adenovirus type-5 COVID-19 vaccine (CanSino Biologics, China). The impact of the Ad5 vector on nAb titers was investigated using 302 blood serum samples from individuals
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New perspective of small-molecule antiviral drugs development for RNA viruses Virology (IF 3.7) Pub Date : 2024-03-07 Shasha Li, Huixia Li, Ruiya Lian, Jingying Xie, Ruofei Feng
High variability and adaptability of RNA viruses allows them to spread between humans and animals, causing large-scale infectious diseases which seriously threat human and animal health and social development. At present, AIDS, viral hepatitis and other viral diseases with high incidence and low cure rate are still spreading around the world. The outbreaks of Ebola, Zika, dengue and in particular of
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Alum/CpG adjuvant promotes immunogenicity of inactivated SARS-CoV-2 Omicron vaccine through enhanced humoral and cellular immunity Virology (IF 3.7) Pub Date : 2024-03-07 Xiaotong Zheng, Rong Yang, Yuxiu Zhao, Yadan Zhang, Guangying Yuan, Weidong Li, Zhuangzhuang Xiao, Xiaofei Dong, Meng Ma, Yancen Guo, Wei Wang, Xue Zhao, Hongqiang Yang, Shaoting Qiu, Zheng Peng, Ankang Liu, Shouzhi Yu, Yuntao Zhang
The SARS-CoV-2 Omicron variant, which was classified as a variant of concern (VOC) by the World Health Organization on 26 November 2021, has attracted worldwide attention for its high transmissibility and immune evasion ability. The existing COVID-19 vaccine has been shown to be less effective in preventing Omicron variant infection and symptomatic infection, which brings new challenges to vaccine
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Saccharomyces cerevisiae cells that display norovirus P induce both systemic and mucosal neutralizing antibodies Virology (IF 3.7) Pub Date : 2024-03-06 Xin He, Nianzhu Jiang, Yaoming Li
The human norovirus (HuNov) is the leading cause of acute gastroenteritis (AGE) worldwide. Mucosal secretory IgA (sIgA) in the gastrointestinal tract interrupts the interaction between host cells and HuNov, thus inhibiting viral infection. In this study, we constructed a recombinant () expressing the HuNov P protein (GII. 4) and evaluated its immunogenicity in mice after oral delivery. First, the recombinant
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Transcriptome analysis of the synergistic mechanisms between two strains of potato virus Y in Solanum tuberosum L. Virology (IF 3.7) Pub Date : 2024-03-06 Liping Xu, Wei Zhang, Shangwu Liu, Yanling Gao, Yuanju Huang, Xianzhou Nie, Yanju Bai
Many viruses employ a process known as superinfection exclusion (SIE) to block subsequent entry or replication of the same or closely related viruses in the cells they occupy. SIE is also referred to as Cross-protection refers to the situation where a host plant infected by a mild strain of a virus or viroid gains immunity against a more severe strain closely related to the initial infectant. The mechanisms
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Distinct tomato yellow leaf curl Chuxiong virus isolated from whiteflies and plants in China and its symptom determinant and suppressor of post-transcriptional gene silencing Virology (IF 3.7) Pub Date : 2024-03-05 Yan Xie, Xianan Liu, Chaohu Luo, Qianqian Hu, Xuan Che, Liling Zhao, Min Zhao, Liqi Wu, Ming Ding
A begomovirus isolated from whiteflies () and tomato, sweet potato in China was found to be representative of a distinct begomovirus species, for which the name tomato yellow leaf curl Chuxiong virus (TYLCCxV) is proposed. The results of genomic identification and sequence comparison showed that TYLCCxV shares the highest complete nucleotide sequence identity (88.3%) with croton yellow vein mosaic
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The conserved RNP motif of the herpes simplex virus 1 family B DNA polymerase is crucial for viral DNA synthesis but not polymerase activity Virology (IF 3.7) Pub Date : 2024-03-03 Jessica L. Lawler, Shariya Terrell, Donald M. Coen
The herpes simplex virus 1 DNA polymerase contains a highly conserved structural motif found in most family B polymerases and certain RNA-binding proteins. To investigate its importance within cells, we constructed a mutant virus with substitutions in two residues of the motif and a rescued derivative. The substitutions resulted in severe impairment of plaque formation, yields of infectious virus,
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A comparative analysis of phage classification methods in light of the recent ICTV taxonomic revisions Virology (IF 3.7) Pub Date : 2024-02-29 Xihui Zhu, Luqi Tang, Zhiwei Wang, Feng Xie, Wei Zhang, Yubao Li
Recent ICTV taxonomy updates significantly changed phage taxonomy, yet a thorough phage classification workflow doesn't exist. This study compares six categorization tools and establishes a novel multi-method approach, combining genome similarity and specialized protein analysis. Applying the method to APEC phage P151 showed consistent categorization across platforms. A possible workflow for phage
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Characterization of Sf9 cell clones with differential susceptibilities to Sf-rhabdovirus X+3.7 and Sf-rhabdovirus X− replication Virology (IF 3.7) Pub Date : 2024-02-27 Hailun Ma, Andrea Kennard, Nicholas Mattson, Arifa S. Khan
Our laboratory previously discovered a novel rhabdovirus in the Sf9 insect cell line that was designated as Sf-rhabdovirus. Using limiting dilution, this cell line was found to be a mixed population of cells infected by Sf-rhabdovirus variants containing either the full length X accessory gene with a 3.7 kb internal duplication (designated as Sf-rhabdovirus X) or lacking the duplication and part of
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Preliminary report on therapeutic potential of coal-derived carbon quantum dots against SARS-CoV-2 virus Virology (IF 3.7) Pub Date : 2024-02-27 Binoy K. Saikia, Kallol Roy, Rituraj Konwar
Due to the pandemic of COVID-19 and subsequent emerging of new mutant strains, there has been a worldwide hunt for therapeutic and protective agents for its inhibition. In this short communication, for the first time, we report the coal-derived carbon quantum dot (CQD) for the possible therapeutic application against SARS-CoV-2. The synthesized C1-CQD is observed to be safe towards the normal cell
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Oncolytic viral therapy for gliomas: Advances in the mechanisms and approaches to delivery Virology (IF 3.7) Pub Date : 2024-02-20 A. Romanishin, A. Vasilev, E. Khasanshin, A. Evtekhov, E. Pusynin, K. Rubina, V. Kakotkin, M. Agapov, E. Semina
Glioma is a diverse category of tumors originating from glial cells encompasses various subtypes, based on the specific type of glial cells involved. The most aggressive is glioblastoma multiforme (GBM), which stands as the predominant primary malignant tumor within the central nervous system in adults. Despite the application of treatment strategy, the median survival rate for GBM patients still hovers
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Analysis of codon usage bias of exonuclease genes in invertebrate iridescent viruses Virology (IF 3.7) Pub Date : 2024-02-19 Yeşim Aktürk Dizman
Invertebrate iridescent viruses (IIVs) are double-stranded DNA viruses that belong to the family. IIVs result diseases that vary in severity from subclinical to lethal in invertebrate hosts. Codon usage bias (CUB) analysis is a versatile method for comprehending the genetic and evolutionary aspects of species. In this study, we analyzed the CUB in 10 invertebrate iridescent viruses exonuclease genes
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Investigation and characterization of rice dwarfing epidemic caused by southern rice black-streaked dwarf virus in Jiangsu in 2023 Virology (IF 3.7) Pub Date : 2024-02-18 Chenyang Li, Wenxuan Yang, Yuanming Zhang, Feng Zhu, Yanglin Qiu, Linlin Du, Feng Lin, Ying Lan, Kai Xu, Tong Zhou
During the field surveys in Jiangsu Province, China, contiguous patches of rice plants with varying degrees of dwarfing, wax-white or dark brown enations at the base of stems, and abnormal heading symptoms were observed in the fields located in Jiangning District in Nanjing City, Jurong County in Zhenjiang City, and Zhangjiagang County in Suzhou City. Through molecular analyses, the presence of southern
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Effect of Enteromorpha polysaccharides on gut-lung axis in mice infected with H5N1 influenza virus Virology (IF 3.7) Pub Date : 2024-02-17 Yanyi Huang, Peiyang Zhang, Shuyi Han, Bin Hu, Qingxun Zhang, Hongxuan He
Enteromorpha polysaccharides (EPPs) have been reported to have antiviral and anti-inflammatory properties. To explore the effect of EPPs on H5N1-infected mice, mice were pretreated with EPPs before being infected with the H5N1 influenza virus intranasally. H5N1 infection resulted in body-weight loss, pulmonary and intestinal damage, and an imbalance of gut microbiota in mice. As a result of the inclusion
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Mucosal vaccines for viral diseases: Status and prospects Virology (IF 3.7) Pub Date : 2024-02-15 Bingjie Ma, Mengxiao Tao, Zhili Li, Quanfang Zheng, Haigang Wu, Peirong Chen
Virus-associated infectious diseases are highly detrimental to human health and animal husbandry. Among all countermeasures against infectious diseases, prophylactic vaccines, which developed through traditional or novel approaches, offer potential benefits. More recently, mucosal vaccines attract attention for their extraordinary characteristics compared to conventional parenteral vaccines, particularly
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Emerging challenges in the management of Orthotospoviruses in Indian agriculture Virology (IF 3.7) Pub Date : 2024-02-15 R.K. Mohana Pradeep, V. Rakesh, N. Boopathi, M. Siva, S. Kousalya, K. Nagendran, G. Karthikeyan
Orthotospoviruses, a genera of negative-sense ssRNA viruses transmitted by thrips, have gained significant attention in recent years due to their detrimental impact on diverse crops, causing substantial economic losses and posing threats to food security. Orthotospoviruses are characterised by a wide range of symptoms in plants, including chlorotic/necrotic spots, vein banding, and fruit deformation
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Development and application of sugarcane streak mosaic virus vectors Virology (IF 3.7) Pub Date : 2024-02-15 Yuteng Yin, Duan Wang, Hongwei Wang, Yanwei Sun, Cece Yin, Jie Li, Jian Ye
Sugarcane streak mosaic virus (SCSMV) is one of the major pathogens of sugarcane in the world. Molecular studies and disease management of SCSMV are hindered by the lack of efficient infectious clones. In this study, we successfully constructed infiltration based infectious clone of SCSMV with different variants. Infectious clones of wild type SCSMV could efficiently infect and sugarcane plants resulting
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Identification of several African swine fever virus replication inhibitors by screening of a library of FDA-approved drugs Virology (IF 3.7) Pub Date : 2024-02-14 Tingting Li, Jun Zheng, Tao Huang, Xiao Wang, Jiangnan Li, Feng Jin, Wenjuan Wei, Xin Chen, Chuanxia Liu, Miaofei Bao, Gaihong Zhao, Li Huang, Dongming Zhao, Jianxin Chen, Zhigao Bu, Changjiang Weng
African swine fever (ASF) caused by African swine fever virus (ASFV) is a highly infectious and lethal swine disease. Currently, there is only one novel approved vaccine and no antiviral drugs for ASFV. In the study, a high-throughput screening of an FDA-approved drug library was performed to identify several drugs against ASFV infection in primary porcine alveolar macrophages. Triapine and cytarabine
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Determination of the three-dimensional structure of bacteriophage Mu(-) tail fiber and its characterization. Virology (IF 3.7) Pub Date : 2024-02-13 Yukina Mori, Eiki Yamashita, Atsushi Nakagawa, Tomoki Matsuzawa, Minoru Inagaki, Yoshifumi Aiba, Syu Tanaka, Syunya Hatori, Maeda Ayami, Shigeki Takeda
Bacteriophage Mu is a phage known to infect various species of Enterobacteria, playing a role in bacterial mutation induction and horizontal gene transfer. The phage possesses two types of tail fibers for host recognition, which enable it to expand its range of hosts. The alternate tail fibers are formed through the action of genes 49–50 or 52–51, allowing the Mu phage to recognize different surfaces
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Development of full-length infectious cDNA clones and host range identification of an echinacea strain of tobacco streak virus Virology (IF 3.7) Pub Date : 2024-02-11 Qi Liu, Menglin Li, Xuanyu Dong, Chengxiao Zuo, Zhangyao Nie, Zongying Zhang, Cheng-Gui Han, Ying Wang
Tobacco streak virus induces severe diseases on a wide range of plants and becomes an emerging threat to crop yields. However, the infectious clones of TSV remain to be developed for reverse genetics studies. Here, we obtained the full genome sequence of a TSV-CNB isolate and analyzed the phylogenetic characteristics. Subsequently, we developed the full-length infectious cDNA clones of TSV-CNB driven
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Distribution patterns and functional diversity of DNA viruses determined by ecological niches in huge river ecosystems Virology (IF 3.7) Pub Date : 2024-02-11 Kang Zhou, Hui Zhou
While the vast number of DNA and RNA viruses participate in biogeochemical cycles in natural systems, little is known about virome in river ecosystems. Here, we analyzed the DNA viral composition and its metabolic potential in the Yangtze River, including freshwater (FW) and freshwater sediments (FWS). A total of 1237 river-derived virus contigs (RVCs) were obtained following de novo assembly from
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Preliminary molecular study for DIVA trial of antigenically characterized circulating bovine herpesvirus subtype 1.1 in Egypt. Virology (IF 3.7) Pub Date : 2024-02-10 Basel A. Abukhadra, Sahar Abd El Rahman, Mohamed A. Soltan, Giuma E. Elhafi, Samah M. Mosad
Using marker vaccines to control bovine alphaherpesvirus-1 (BoHV-1) is a novel strategy for differentiation between infected and vaccinated animals (DIVA). In this study, multiplex real-time PCR targeting gD and gE genes was applied for BoHV-1 screening on 60 clinical samples from cattle with a history of vaccination, in some cases by US2-deleted marker vaccines, that were suffering from severe respiratory
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p53 suppresses the inflammatory response following respiratory syncytial virus infection by inhibiting TLR2 Virology (IF 3.7) Pub Date : 2024-02-10 Jiao Liu, Leiqiong Gao, Na Zhou, Zhenghong Jiang, Siyi Che, Yu Deng, Na Zang, Luo Ren, Xiaohong Xie, Jun Xie, Enmei Liu
—Respiratory syncytial virus (RSV) is a pivotal virus leading to acute lower respiratory tract infections in children under 5 years old. This study aimed to explore the correlation between p53 and Toll-like receptors (TLRs) post RSV infection. p53 levels exhibited a substantial decrease in nasopharyngeal aspirates (NPAs) from infants with RSV infection compared to control group. Manipulating p53 expression
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Genome analysis of a novel avian atadenovirus reveals a possible horizontal gene transfer Virology (IF 3.7) Pub Date : 2024-02-08 Weibo Zheng, Xiaopeng Teng, Tingshu Jiang, Wenli Tang, Linlin Jiang, Hongwei Zhu, Xin Yu, Guozhong Chen, Jiao Wang, Jianlong Zhang, Mingjuan Qu, Xingxiao Zhang
We report the discovery and characterization of a novel adenovirus, Zoothera dauma adenovirus (ZdAdV), from a wild bird species, (Scaly thrush). This new atadenovirus was discovered by metagenomic sequencing without virus cultivation. Analyses of the full genome sequence revealed that this new virus is a distinct member of the genus and represents a novel species. ZdAdV has a genome of 34,760 bp with
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Australian terrestrial environments harbour extensive RNA virus diversity Virology (IF 3.7) Pub Date : 2024-02-08 Sabrina Sadiq, Erin Harvey, Jonathon C.O. Mifsud, Budiman Minasny, Alex B. McBratney, Liana E. Pozza, Jackie E. Mahar, Edward C. Holmes
Australia is home to a diverse range of unique native fauna and flora. To address whether Australian ecosystems also harbour unique viruses, we performed meta-transcriptomic sequencing of 16 farmland and sediment samples taken from the east and west coasts of Australia. We identified 2460 putatively novel RNA viruses across 18 orders, the vast majority of which belonged to the microbe-associated phylum
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Meta-transcriptomic identification of groundnut RNA viruses in western Kenya and the novel detection of groundnut as a host for Cauliflower mosaic virus Virology (IF 3.7) Pub Date : 2024-02-06 Dennis Obonyo, George Ouma, Rachel Ikawa, Damaris A. Odeny
Groundnut ( L.) is the 13th most important global crop grown throughout the tropical and subtropical regions of the world. One of the major constraints to groundnut production is viruses, which are also the most economically important and most abundant pathogens among cultivated legumes. Only a few studies have reported the characterization of RNA viruses in cultivated groundnuts in western Kenya,
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Engineering of stable infectious cDNA constructs of a fluorescently tagged tomato chlorosis virus Virology (IF 3.7) Pub Date : 2024-02-06 Sun-Jung Kwon, Ye-Ji Lee, Young-Eun Cho, Hee-Seong Byun, Jang-Kyun Seo
Tomato chlorosis virus (ToCV) is an emerging pathogen that cause severe yellow leaf disorder syndrome in tomato plants. In this study, we aimed to generate a recombinant ToCV tagged with green fluorescent protein (GFP) to enable real-time monitoring of viral infection in living plants. Transformation of the full-length cDNA construct of ToCV RNA1 into resulted in instability issues, which were successfully
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Proprotein convertase cleavage of Ictalurid herpesvirus 1 spike-like protein ORF46 is modulated by N-glycosylation Virology (IF 3.7) Pub Date : 2024-02-06 Fei Yu, Jiehua Xu, Hongxun Chen, Siyang Song, Chunlan Nie, Kai Hao, Zhe Zhao
Viral spike proteins undergo a special maturation process that enables host cell receptor recognition, membrane fusion, and viral entry, facilitating effective virus infection. Here, we investigated the protease cleavage features of ORF46, a spike-like protein in 1 (IcHV-1) sharing similarity with spikes of Nidovirales members. We noted that during cleavage, full-length ORF46 is cleaved into ∼55-kDa
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Isolation and characterization of genotype 4 Eurasian avian-like H1N1 influenza virus in pigs suffering from pneumonia Virology (IF 3.7) Pub Date : 2024-02-02 Jizhe Yang, Riguo Lan, Haoyu Chang, Han Li, Haili Yu, Qi Tong, Jinhua Liu, Honglei Sun
Swine influenza viruses pose ongoing threat to pork industry throughout the world. In 2023, fattening pigs from a swine farm in Inner Mongolia of China experienced influenza-like symptoms. Co-infection of influenza A virus with was diagnosed in lung tissues of diseased pigs and a genotype 4 (G4) Eurasian avian-like (EA) H1N1 virus was isolated, which was named as A/swine/Neimenggu/0326/2023. We demonstrated
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A cell-adapted SARS-CoV-2 mutant, showing a deletion in the spike protein spanning the furin cleavage site, has reduced virulence at the lung level in K18-hACE2 mice. Virology (IF 3.7) Pub Date : 2024-01-28 Fabrizia Valleriani, Chiara Di Pancrazio, Massimo Spedicato, Giovanni Di Teodoro, Daniela Malatesta, Tetyana Petrova, Francesca Profeta, Maria Loredana Colaianni, Shadia Berjaoui, Ilaria Puglia, Marialuigia Caporale, Emanuela Rossi, Maurilia Marcacci, Mirella Luciani, Flavio Sacchini, Ottavio Portanti, Francesco Bencivenga, Nicola Decaro, Francesco Bonfante, Alessio Lorusso
Here we investigated the virulence properties of a unique cell-adapted SARS-CoV-2 mutant showing a ten-amino acid deletion encompassing the furin cleavage site of the spike protein (Δ680SPRAARSVAS689; Δ680-689-B.1) in comparison to its parental strain (wt-B.1) and two Delta variants (AY.122 and AY.21). After IN inoculation, transgenic K18-hACE2 mice were monitored for 14 days for weight change, lethality
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Application of nanoparticles for management of plant viral pathogen: Current status and future prospects Virology (IF 3.7) Pub Date : 2024-01-26 Ashish Warghane, Rashmi Saini, Manju Shri, Isha Andankar, Dilip Kumar Ghosh, Balu Ananda Chopade
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Comparative analysis and prediction of avian influenza in Shangrao city, China from 2016 to 2022 Virology (IF 3.7) Pub Date : 2024-01-24 Kang Fang, Wentao Song, Yanyan Zhang, Yiyang Zheng, Chen You, Jianhai Hu, Li Liu, Lei Feng, Zeyu Zhao, Yunkang Zhao, Jiayi Wang, Xiaolan Wang, Lin Zhu, Tianmu Chen
The aim of this study was to investigate the effects of vaccination, COVID-19 pandemic and migration of migratory birds on the avian influenza positivity rate in Shangrao City and to predict the future avian influenza positivity rate. Real-time reverse transcription polymerase chain reaction (RT-PCR) was used to detect nucleic acids of avian influenza A viruses. 1795 samples were collected between
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Effect of chemical and physical agents on monkeypox virus infectivity and downstream research applications Virology (IF 3.7) Pub Date : 2024-01-16 Davide Mariotti, Aurora Bettini, Silvia Meschi, Stefania Notari, Massimo Francalancia, Eleonora Tartaglia, Daniele Lapa, Eliana Specchiarello, Enrico Girardi, Giulia Matusali, Fabrizio Maggi
The 2022 global spread of Monkeypox Virus (MPXV) underlined the need to investigate safe-handling procedures of clinical and research samples. Here we evaluated the efficiency in reducing MPXV infectious titer of Triton X-100 (0.1 and 0.2%), UV-C irradiation (15 or 30 min), and heat (56 °C 30 min or 70 °C 5 min). The treatment of MPXV at 70 °C resulted in the strongest decrease of MPXV infectious titer