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  • The development and characterisation of gallium doped bioactive glasses for potential bone cancer applications.
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-22
    Karan Rana, Lucas Souza, Mark A. Isaacs, Farah Raja, Alexander Paul Morrell, Richard Alan Martin

    In this study we have developed a series of novel gallium oxide doped bioactive glasses to specifically target osteosarcoma cells whilst aiding new bone formation. The results show that osteosarcoma (Saos-2) cell death is induced through the addition of gallium oxide. Relative to the gallium-free control glass (0% Ga) glasses containing 1, 2 and 3% Ga decreased Saos-2 cell viability in a dose dependant manner. After 72 hours in media preconditioned with 3% Ga Saos-2 cell viability was reduced by over 50%. Corresponding studies undertaken on primary normal human osteoblast cells (NHOst) demonstrated no adverse effects to the gallium containing glasses. Hydroxyapatite formation was observed for all glasses when exposed to simulated body fluid.

    更新日期:2017-09-23
  • Incrementing the Frequency of Dynamic Strain on SMC-Cellularised Collagen-Based Scaffolds Affects Extracellular Matrix Remodeling and Mechanical Properties
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-22
    L. Lévesque, C. Loy, A. Lainé, B. Drouin, P. Chevallier, D. Mantovani
    更新日期:2017-09-23
  • Mesenchymal Stem Cells Engineering: Microcapsules-Assisted Gene Transfection and Magnetic Cell Separation
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-22
    Albert R. Muslimov, Alexander S. Timin, Aleksandra V. Petrova, Olga S. Epifanovskaya, Alena I. Shakirova, Kirill V. Lepik, Andrey Gorshkov, Eugenia V. Il’inskaja, Andrey V. Vasin, Boris V. Afanasyev, Boris Fehse, Gleb B. Sukhorukov
    更新日期:2017-09-23
  • 更新日期:2017-09-23
  • Overcoming Multidrug Resistance through the Synergistic Effects of Hierarchical pH-Sensitive, ROS-Generating Nanoreactors
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-22
    Ranjith Kumar Kankala, Chen-Guang Liu, Ai-Zheng Chen, Shi-Bin Wang, Pei-Yao Xu, Lokesh Kumar Mende, Chen-Lun Liu, Chia-Hung Lee, Yu-Fang Hu
    更新日期:2017-09-23
  • Antitumor Photodynamic Therapy Based on Dipeptide Fibrous Hydrogels with incorporation of Photosensitive Drugs
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-22
    Manzar Abbas, Ruirui Xing, Ning Zhang, Qianli Zou, Xuehai Yan

    Hydrogels self-assembled by biologically-based building blocks including peptides and proteins can be used as an ideal drug delivery platform and present great promise for biomedical applications. Herein, photodynamic antitumor therapy based on injectable Fmoc-FF/PLL hydrogels was achieved by encapsulation of the photosensitive drug Chlorin e6 (Ce6), as the hydrogels exhibit multiple favorable therapeutic features, including shear-thinning and self-healing properties, good biocompatibility, and perfect biodegradability. Such injectable hydrogels are shown to be well suited for local injection and sustained drug delivery at the tumor site, especially towards therapy of superficial tumors. In vivo therapeutic results show, that the tumor growth can be efficiently inhibited, especially under the strategy “once injection, multiple-treatments”. During the whole treatment period, no detectable toxicity or damages to normal organs are observed. Therefore, the injectable hydrogels can be applied as a promising delivery platform for therapy of superficial tumors.

    更新日期:2017-09-22
  • Fibronectin in Layer-by-Layer Assembled Films Switches Tumor Cells between 2D and 3D Morphology
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-21
    Kiran Bhadriraju, Jennifer S. Hong, Steven P. Lund, Darwin R. Reyes
    更新日期:2017-09-21
  • Tumor Neovasculature-Targeted APRPG-PEG-PDLLA/MPEG-PDLLA Mixed Micelle Loading Combretastatin A-4 for Breast Cancer Therapy
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-21
    Ying Hao, Jinrong Peng, Yaguang Zhang, Lijuan Chen, Feng Luo, Cheng Wang, Zhiyong Qian
    更新日期:2017-09-21
  • Redox-Responsive Biodegradable Polycation Poly(amido amine) Used As Intranasal Vaccine Delivery Systems
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-20
    Zhong Guo, Sha Li, Meng Lv, Zonghua Liu, Wei Xue
    更新日期:2017-09-20
  • Magnetic resonance imaging of alginate beads containing pancreatic beta cells and paramagnetic nanoparticles
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-19
    Sary Sarkis, Fanny Marie Silencieux, Karen E Markwick, Marc-André Fortin, Corinne Annette Hoesli

    Microencapsulation is being investigated as a means to avoid rejection of transplanted pancreatic islets. Monitoring bead distribution and stability in vivo is an important step towards improving microencapsulated islet transplantation strategies. Islet co-encapsulation with gadolinium-labeled mesoporous silica nanoparticles (Gd-MSNs) could provide a means to visualise the beads while immobilizing and limiting the potential internalisation of the contrast agent. The porous nature of the MSNs could also be used to locally release anti inflammatory, angiogenic, or anti apoptotic factors. Mouse insulinoma 6 (MIN6) beta cells were co-encapsulated with Gd-MSNs in alginate beads produced by emulsification and internal gelation. Gd-MSN alginate beads appeared brighter in T1-w. imaging sequences (detection threshold of 0.016 mM Gd; relaxometric ratio r2/r1 = 1.45). No leaching of Gd3+ from the hydrogels was detected over the course of 3 months. Cells co-encapsulated with Gd-MSNs were viable without significant differences in cell growth rate compared to controls. This study paves the way towards microencapsulated islet tracking via MRI using co-encapsulated paramagnetic nanomaterials.

    更新日期:2017-09-20
  • A Bioinformatics 3D Cellular Morphotyping Strategy for Assessing Biomaterial Scaffold Niches
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-19
    Stephen J. Florczyk, Mylene Simon, Derek Juba, P. Scott Pine, Sumona Sarkar, Desu Chen, Paula J. Baker, Subhadip Bodhak, Antonio Cardone, Mary C. Brady, Peter Bajcsy, Carl G. Simon
    更新日期:2017-09-19
  • Hierarchically Porous Calcium Carbonate Scaffolds for Bone Tissue Engineering
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-18
    Abiy D. Woldetsadik, Sudhir K. Sharma, Sachin Khapli, Ramesh Jagannathan, Mazin Magzoub
    更新日期:2017-09-18
  • Rapid Customization of 3D Integrated Microfluidic Chips via Modular Structure-Based Design
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-15
    Jingjiang Qiu, Qing Gao, Haiming Zhao, Jianzhong Fu, Yong He
    更新日期:2017-09-15
  • Single Dose of Protein Vaccine with Peptide Nanofibers As Adjuvants Elicits Long-Lasting Antibody Titer
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-15
    Chengbiao Yang, Fang Shi, Can Li, Youzhi Wang, Ling Wang, Zhimou Yang
    更新日期:2017-09-15
  • Supramolecular Vaccine Platform Based on α-Helical Peptide Nanofibers
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-15
    Yaoying Wu, Pamela K. Norberg, Elizabeth A. Reap, Kendra L. Congdon, Chelsea N. Fries, Sean H. Kelly, John H. Sampson, Vincent P. Conticello, Joel H. Collier
    更新日期:2017-09-15
  • Supramolecular Hydrogel Inspired from DNA Structures Mimics Peroxidase Activity
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-15
    Tanima Bhattacharyya, Y. Pavan Kumar, Jyotirmayee Dash

    We herein report that hydrogels can be prepared from guanosine and boronic acids in the presence of K+ and Pb2+. These supramolecular hydrogels are formed via G-quartet like self-assembly of guanosine and its boronate esters. The potential of this hydrogel construct in mimicking enzyme-like activity has been demonstrated for the first time. We have observed that the self-assembled structure present in K+ stabilized hydrogel binds to iron (III)-hemin and shows peroxidase activity, catalyzing oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB) in the presence of H2O2. Furthermore, the conformation of the G-quartet assemblies in the hydrogel can be altered by varying the stabilizing cations K+ and Pb2+. This conformational switching has been used to devise a molecular logic gate for sensing of toxic Pb2+ ions.

    更新日期:2017-09-15
  • Population Effects of Calcium Phosphate Nanoparticles in Drosophila melanogaster: The Effects of Phase Composition, Crystallinity and the Pathway of Formation
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-13
    Victoria Wu, Vuk Uskokovic

    Unpredictable biological response due to finest nanostructural variations is one of the hallmarks of nanoparticles. Because of this erratic behavior of nanoparticles in living systems, thorough analyses of biosafety must precede the analyses of the pharmacotherapeutic efficacy and simple animal models are ideal for such purposes. Drosophila melanogaster, the common fruit fly, is an animal model capable of giving a fast, high-throughput response as to the safety and efficacy of drug delivery carriers and other pharmacological agents, while minimizing the suffering imposed onto animals in more complex in vivo models. Here we studied the effects on the viability and fertility of D. melanogaster due to variations in phase composition, crystallinity and the pathway of formation of four different calcium phosphate (CP) nanopowders consumed orally. To minimize the effect of other nanostructural variables, CP nanopowders were made to possess highly similar particle sizes and morphologies. The composition of CP affected the fecundity of flies, but so did crystallinity and the pathway of formation. Both the total number of eclosed viable flies and pupae in populations challenged with hydroxyapatite (HAP) greatly exceeded those in control populations. Viability was adversely affected by the only pyrophosphate tested (CPP) and by the metastable and the most active of all CP nanopowders analyzed: the amorphous CP (ACP). The pupation peak was delayed and the viable fly-to-pupa ratio increased in all the CP-challenged populations. F1 CPP population, whose viability was most adversely affected by the CP consumption, when crossed, produced the largest number of F2 progeny under regular conditions, possibly pointing to stress as a positive evolutionary drive. The positive effect of HAP on fertility of fruit flies may be due to its slow absorption and the activation of calmodulin during the transit of oocytes through the reproductive tract of fertilized females. Exerted in the pre-pupation stage, the effect of CP is thus traceable beyond the instar larval stage and to the oogenesis stage of the Drosophila lifecycle.

    更新日期:2017-09-14
  • Oligo(p-phenylenevinylene) Derivative-Incorporated and Enzyme-Responsive Hybrid Hydrogel for Tumor Cell-Specific Imaging and Activatable Photodynamic Therapy
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-13
    Meng Li, Ping He, Shengliang Li, Xiaoyu Wang, Libing Liu, Fengting Lv, Shu Wang

    Tumor-cell targeted and subcellular organelles-specific photosensitizer is imperative for high-efficiency photodynamic therapy (PDT). Herein, we designed and synthesized a new conjugated oligomer, oligo(p-phenylenevinylene-co-benzothiazole) (OPVBT), which could be used as photosensitizer to generate reactive oxygen species (ROS) upon light illumination. Advantageously, OPVBT was found to specifically accumulate in mitochondria, which is benefit for effective subcellular organelles-targeted PDT. A biocompatible and biodegradable hydrogel was constructed based on Michael-type addition between cysteine-terminated matrix metalloproteinase (MMP)-cleavable oligopeptides and 4-arm vinyl sulfone-terminated poly (ethylene glycol) (PEG-VS). By functionalizing hydrogel with OPVBT and cell adhesion peptide motif RGD, cell attachment and spreading on the hydrogel were easily realized. More importantly, owing to the MMP-sensitivity of the hydrogel, tumor cells could be specifically lighted up by OPVBT liberated from the hydrogel and the growth of tumor cells are further restrained by ROS generated from OPVBT upon light illumination. The hybrid hydrogel provides a new platform for tumor-specific imaging and killing, which shows good potential for constructing targeted PDT system with reduced side effects.

    更新日期:2017-09-14
  • Immobilization of Recombinant E. coli Cells in a Bacterial Cellulose–Silk Composite Matrix To Preserve Biological Function
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-13
    Irina Drachuk, Svetlana Harbaugh, Ren Geryak, David L. Kaplan, Vladimir V. Tsukruk, Nancy Kelley-Loughnane
    更新日期:2017-09-13
  • In Vitro Generation of Mouse Colon Crypts
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-13
    Yuli Wang, Dulan B. Gunasekara, Peter J. Attayek, Mark I. Reed, Matthew DiSalvo, Daniel L. Nguyen, Johanna S. Dutton, Michael S. Lebhar, Scott J. Bultman, Christopher E. Sims, Scott T. Magness, Nancy L. Allbritton
    更新日期:2017-09-13
  • Collagen Fibril Intermolecular Spacing Changes with 2-Propanol: A Mechanism for Tissue Stiffness
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-13
    Hannah C. Wells, Katie H. Sizeland, Susyn J.R. Kelly, Nigel Kirby, Adrian Hawley, Stephen Mudie, Richard G. Haverkamp
    更新日期:2017-09-13
  • Silver Nanowire Particle Reactivity with Human Monocyte-Derived Macrophage Cells: Intracellular Availability of Silver Governs Their Cytotoxicity
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-13
    Ioannis G. Theodorou, Karin H. Müller, Shu Chen, Angela E. Goode, Vladimir Yufit, Mary P. Ryan, Alexandra E. Porter
    更新日期:2017-09-13
  • Review: Femtosecond-laser-based 3D Printing for Tissue Engineering and Cell Biology Applications
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-13
    Chee Meng Benjamin Ho, Abhinay Mishra, Kan Hu, Jianing An, Young-Jin Kim, Yong-Jin Yoon

    Fabrication of 3D cell scaffolds has gained tremendous attention in recent years due to its applications in tissue engineering and cell biology applications. The success of tissue engineering or cell interactions mainly depends on the fabrication of well-defined microstructures, which ought to be biocompatible for cell proliferation. Femtosecond-laser-based 3D printing is one of the solution candidates that can be used to manufacture 3D tissue scaffolds through computer-aided design (CAD) which can be efficiently engineered to mimic the microenvironment of tissues. UV-based lithography has also been used for constructing the cellular scaffolds but the toxicity of UV light to the cells has prevented its application to the direct patterning of the cells in the scaffold. Although the mask-based lithography has provided a high resolution, it has only enabled 2D patterning not arbitrary 3D printing with design flexibility. Femtosecond-laser-based 3D printing is trending in the area of tissue engineering and cell biology applications due to the formation of well-defined micro- and sub-micrometer structures via visible and near infrared (NIR) femtosecond laser pulses, followed by the fabrication of cell scaffold microstructures with a high precision. Laser direct writing and multi-photon polymerization are being used for fabricating the cell scaffolds, The implication of spatial light modulators in the interference lithography to generate the digital hologram will be the future prospective of mask-based lithography. Polyethylene glycol diacrylate (PEG-DA), ormocomp, pentaerythritol tetraacrylate (PETTA) have been fabricated through two-photon polymerization to generate the cell scaffolds, while SU-8 was used to fabricate the microrobots for targeted drug delivery. Well-designed and precisely fabricated 3D cell scaffolds manufactured by femtosecond-laser-based 3D printing can be potentially used for studying cell migration, matrix invasion and nuclear stiffness to determine stage of cancer and will open broader horizons in the future in tissue engineering and biology applications.

    更新日期:2017-09-13
  • Amphiphilic Near-Infrared Conjugated Polymer for Photothermal and Chemo Combination Therapy
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-12
    Jingke Yao, Shusen Kang, Jian Zhang, Jia Du, Zhe Zhang, Mao Li
    更新日期:2017-09-12
  • Rational Design of Peptide-Functionalized Poly(Methacrylic Acid) Brushes for On-Chip Detection of Protease Biomarkers
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-12
    Yeping Wu, Muhammad Naeem Nizam, Xiaokang Ding, Fu-Jian Xu
    更新日期:2017-09-12
  • Morphology-Controlled Synthesis of Lignin Nanocarriers for Drug Delivery and Carbon Materials
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-12
    Doungporn Yiamsawas, Sebastian J. Beckers, Hao Lu, Katharina Landfester, Frederik R. Wurm
    更新日期:2017-09-12
  • Injectable and Degradable Poly(Oligoethylene glycol methacrylate) Hydrogels with Tunable Charge Densities as Adhesive Peptide-Free Cell Scaffolds
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-12
    Emilia Bakaic, Niels M.B. Smeets, Maryam Badv, Megan Dodd, Owen Barrigar, Emily Siebers, Michael Lawlor, Heather Sheardown, Todd Hoare
    更新日期:2017-09-12
  • “Dandelion” Inspired Dual-Layered Nanoarrays with Two Model Releasing Features for the Surface Modification of 3D Printing Implants
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-11
    Xinxin Miao, Hang Liao, Zhongbo Deng, Chen Li, Tianlong Wu, Huiqing Zhang, Miaoxing Liu, Xigao Cheng, Xiaolei Wang
    更新日期:2017-09-11
  • Surface Engineering for Cell-based Therapies: Techniques for Manipulating Mammalian Cell Surfaces
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-11
    Srinivas Abbina, Erika Siren, Haisle Moon, Jayachandran N Kizhakkedathu

    The introduction of cell-based therapies has provided new and unique strategies to treat many diseases and disorders including the recent approval of CAR-T cell therapy for the leukemia. Cell surface engineering is a methodology in which the cell surface is tailored to modulate cellular function and interactions. In addition to genetic engineering of cell surface proteins, a wide array of robust, innovative and elegant approaches have been developed to selectivity target the cell surface. In this review, we will introduce the leading strategies currently used in cell surface engineering including broadly reactive chemical ligations and physical associations as well as more controlled approaches as demonstrated in genetic, enzymatic and metabolic engineering. Prominent applications of these strategies for cell-based therapies will be highlighted including targeted cell death, controlling stem cell fate, immunoevasion, blood transfusion and the delivery of cells to target tissues. Advances will be focused specifically on cells which are the most promising in generating cell-based therapeutics including red blood cells, white blood cells (lymphocytes, macrophages), stem cells (multipotent and pluripotent), islet cells, cancer cells and endothelial cells.

    更新日期:2017-09-11
  • A supramolecular vaccine platform based on alpha-helical peptide nanofibers
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-11
    Yaoying Wu, Pamela K Norberg, Elizabeth A Reap, Kendra L Congdon, Chelsea N Fries, Sean H Kelly, John Sampson, Vince Conticello, Joel H. Collier

    A supramolecular peptide vaccine system was designed in which epitope-bearing peptides self-assemble into elongated nanofibers composed almost entirely of alpha-helical structure. The nanofibers were readily internalized by antigen presenting cells and produced robust antibody, CD4+ T-cell, and CD8+ T-cell responses without supplemental adjuvants in mice. Epitopes studied included a cancer B-cell epitope from the epidermal growth factor receptor class III variant (EGFRvIII), the universal CD4+ T-cell epitope PADRE, and the model CD8+ T-cell epitope SIINFEKL, each of which could be incorporated into supramolecular multi-epitope nanofibers in a modular fashion.

    更新日期:2017-09-11
  • Bioerosion of Synthetic Sling Explants
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-11
    Katherine E. Washington, Gina Quiram, Angela V Nguyen, Ruvanthi N. Kularatne, Majid Minary-Jolandan, Philippe Zimmern, Mihaela C. Stefan

    This study was performed to investigate the changes over time in polypropylene (PP) mesh explants from women with stress urinary incontinence originally treated with a mid-urethral PP sling. Following Institutional Review Board (IRB) approval, 10 PP explants removed for pain or obstructive symptoms between January and June 2016 were analyzed through various techniques to determine the degradation of the material in vivo. Exclusion criteria were exposed or infected mesh sling, or sling in place for less than 6 months. One pristine control was studied for comparison. The explant samples were analyzed with scanning electron microscopy (SEM) to visualize the surface defects as well as infrared spectroscopy (IR) and energy dispersive X-ray spectroscopy (EDS) to determine if the degradation was oxidative in nature. The results show qualitative and quantitative bioerosion over the surface of the explant samples and an increase in the content of oxygen pointing towards oxidative degradation occurring in vivo.

    更新日期:2017-09-11
  • Incrementing the frequency of dynamic strain on SMC-cellularised collagen-based scaffolds affects extracellular matrix remodeling and mechanical properties
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-08
    Lucie Lévesque, Caroline Loy, Audrey Lainé, Bernard Drouin, Pascale Chevallier, Diego Mantovani

    Notwithstanding the efforts injected in vascular tissue engineering in the past thirty years, the clinical translation of engineered artery constructs is far from being successful. One common approach to improve artery regeneration is the use of cyclic mechanical stimuli to guide cellular remodeling. However, there is a lack of information on the effect of cyclic strain on cells within a 3D environment. To this end, this work explored the effect of gradual increase in stimulation frequency on the response of human umbilical artery smooth muscle cells (HUASMCs) embedded in a 3D collagen matrix. The results demonstrate that, with an applied strain of 5%, the gradual increase of frequency from 0.1 Hz to 1 Hz improved collagen remodeling by HUASMCs compared to samples constantly stimulated at 1 Hz. The expression of collagen, elastin and matrix metalloproteinase-2 (MMP-2) genes was similar at 7 days for gradual and 1 Hz samples which showed lower amounts than static counterparts. Interestingly the mechanical properties of the constructs, specifically the amplitude of the time constants and the elastic equilibrium modulus, were enhanced by gradual increase of frequency. Taken together, these results show an increase in collagen remodeling by the HUASMCs overtime to incremental cyclic mechanical strain. This work suggests that only the in-depth investigation of the effects of stimulation parameters on the behaviour of vSMC under cyclic strain in a 3D environment could lead to the design of optimised control strategies for enhanced vascular tissue generation and maturation in bioreactors.

    更新日期:2017-09-08
  • Combined chemo-photothermal anti-tumor therapy using molybdenum disulfide modified with hyperbranched polyglycidyl
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-07
    Kewei Wang, Qianqian Chen, Wei Xue, Sha Li, Zonghua Liu

    In the treatment of cancers, molybdenum disulfide (MoS2) has shown great potential as a photo absorbing agent in photothermal therapy and meanwhile as an anti-tumor drug delivery system in chemotherapy. However, the poor dispersibility and stability of MoS2 in aqueous solutions limit its applications in cancer therapy. To overcome the shortcomings, MoS2 was modified mainly by surface adsorption of linear polymers, such as chitosan and polyethylene glycol. As reported, the linear polymers could be more rapidly cleared from blood circulation than their branched counterparts. Herein, we developed hyperbranched polyglycidyl (HPG)-modified MoS2 (MoS2-HPG) by absorbing HPG on the MoS2 surface. The MoS2-HPG as a novel photo absorbing agent was also used as a nano-scaled carrier to load anti-tumor drug doxorubicin hydrochloride (DOX) (MoS2-HPG-DOX) for combined chemo-photothermal therapy. The physicochemical and photothermal properties of MoS2-HPG were measured, and the results indicate that MoS2-HPG had good dispersion and stability in aqueous solutions, and also high photothermal conversion efficiency. MoS2-HPG displayed good biocompatibility in hemocompatibility and cytotoxicity evaluations in vitro. Furthermore, the combined chemo-photothermal therapy by using MoS2-HPG-DOX demonstrated better anti-cancer effect than the individual chemotherapy or photothermal therapy alone. From the results, MoS2-HPG with combined chemo-photothermal therapy could be developed as a promising therapeutic formulation for clinical cancer treatment.

    更新日期:2017-09-08
  • Polypeptide-Engineered Hydrogel Coated Gold Nanorods for Targeted Drug Delivery and Chemo-photothermal Therapy
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-07
    Jie Yang, Ming-Hao Yao, Rui-Mei Jin, Dong-Hui Zhao, Yuan-Di Zhao, Bo Liu
    更新日期:2017-09-07
  • Overcoming multidrug resistance through the synergistic effects of hierarchical pH-sensitive, ROS-generating Nanoreactors
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-07
    Ranjith Kumar Kankala, Chen-Guang Liu, Ai-Zheng Chen, Shi-Bin Wang, Peiyao Xu, Lokesh Kumar Mende, Chen-Lun Liu, Chia-Hung Lee, Yu-Fang Hu

    Recently, multi-drug resistance (MDR) has become a major clinical chemotherapeutic burden that robustly diminishes the intracellular drug levels through various mechanisms. To overcome the doxorubicin (Dox) resistance in tumor cells, we designed a hierarchical nanohybrid system possessing copper-substituted mesoporous silica nanoparticles (Cu-MSNs). Further, Dox was conjugated to copper metal in the Cu-MSNs framework through a pH-sensitive coordination link, which is acutely sensitive to the tumor acidic environment (pH-5.0-6.0). In the end, the nanocarrier was coated with D-α-Tocopherol polyethylene glycol 1000 succinate (TPGS), a P-gp inhibitor-entrenched compact liposome net for obstructing the drug efflux pump. Copper ions in the framework synergize the anti-tumor activity of Dox by enhancing the intracellular reactive oxygen species (ROS) levels through a Fenton-like reaction-mediated conversion of hydrogen peroxide. Furthermore, intracellularly-generated ROS triggered the apoptosis by reducing the cellular as well as mitochondrial membrane integrity in MDR cells, which was confirmed by the mitochondrial membrane potential (MMP) measurement. The advancement of the design and critical improvement of cytotoxic properties through free radical attack demonstrate that the proposed hierarchical design can devastate the MDR for efficient cancer treatment.

    更新日期:2017-09-07
  • Highly Bioadhesive Polymer Membrane Continuously Releases Cytostatic and Anti-Inflammatory Drugs for Peritoneal Adhesion Prevention
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-07
    Jiannan Li, Weiguo Xu, Jinjin Chen, Di Li, Kai Zhang, Tongjun Liu, Jianxun Ding, Xuesi Chen

    Peritoneal adhesion is a complex fibrosis and inflammatory process, and it can be minimized by physical isolation by biomaterial membranes or treatment with cytostatic and/or anti-inflammatory drugs. However, the integration of physical isolation and pharmaceutical therapy in one platform faces many challenges. First, normal polymer anti-adhesion membranes are hydrophobic and with low bioadhesion to the injured tissue, which decrease their efficacies. Second, the significantly different release behaviors of various drugs owing to their different hydrophilic/hydrophobic properties limit their synergistic effects. In this study, a highly bioadhesive polymer membrane formed by core−sheath nanofiber to integrate physical isolation and pharmaceutical treatment together for the synergistic prevention of peritoneal adhesion. 10-Hydroxycamptothecin (HCPT) and diclofenac sodium (DS) were loaded in the sheath and core of nanofiber, respectively. The membrane was then treated by ultraviolet-ozone (UVO) for improvement of hydrophilicity and bioadhesion. Owing to the core−sheath structure, the two drugs both performed a sustained release behavior for the cytostatic and anti-inflammatory effects. The in vivo study demonstrated that the UVO-treated and dual-drug-coloaded membrane possessed the best anti-adhesion capacity, indicating its potential clinical application.

    更新日期:2017-09-07
  • Enhanced Osteogenic Commitment of Human Mesenchymal Stem Cells on Polyethylene Glycol-Based Cryogel with Graphene Oxide Substrate
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-07
    Hwan D. Kim, Jiyong Kim, Rachel H. Koh, Jimin Shim, Jong-Chan Lee, Tae-Il Kim, Nathaniel S. Hwang
    更新日期:2017-09-07
  • Amphiphilic Near-Infrared Conjugated Polymer for Photo-thermal and Chemo- Combination Therapy
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-06
    Jingke Yao, Shusen Kang, Jian Zhang, Jia Du, Zhe Zhang, Mao Li

    Amphiphilic conjugated polymer was designed and utilized as nanocarriers without further general encapsulation using PEGylated materials for photothermal therapy (PTT) and chemotherapy. These nanocarriers have maximum absorption in ideal phototherapeutic window between 800 and 850 nm and excellent photothermal conversion efficiency of 76% at 808 nm. It provides the simultaneous therapy of chemotherapy and PTT with the monitoring of photoacoustic imaging. After combined therapy via tail vein injection, complete remission and no recurrence of tumors can be observed over a course of 20 days, indicating these amphiphilic NPs has great potential for NIR photoacoustic imaging-guided photothermal and chemo- combined therapy.

    更新日期:2017-09-07
  • Redox-responsive biodegradable polycation poly(amido amine) used as intranasal vaccine delivery systems
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-06
    Zhong Guo, Sha Li, Meng Lv, Zonghua Liu, Wei Xue

    Polycations such as polyethyleneimine and chitosan have been widely used as mucosal vaccine delivery systems due to their permeation enhancement effect. Preferably, environmentally-responding biodegradable polycations would be better carrier materials for mucosal vaccine delivery. Disulfide bonds-based redox-sensitive polycations could respond to the higher intracellular glutathione concentration and degrade in the cytoplasm via the breakage of the disulfide bonds, which are particularly suitable for antigen delivery. In this work, we evaluated the potential of redox-sensitive, biodegradable polycation poly(amido amine) (PAA) as mucosal vaccine carriers. From the primary studies with ovalbumin used as a model protein antigen, it is found that PAA could complex with and encapsulate protein antigen via electrostatic attraction, enhance the cellular uptake of antigen by dendritic cell line DC2.4, prolong antigen residence in nasal cavity, and promote antigen permeation into nasal submucosal layer. Further, Balb/c mice were intranasally immunized with PAA-delivered recombinant hemagglutinin (HA) antigen protein of H7N9 influenza virus. The PAA/HA formulations induced significantly more potent systemic IgG response and mucosal IgA response, higher splenocyte proliferation activity, higher secretion levels of cytokines IFN-γ and IL-4 by splenocytes, more memory CD4+ and CD8+ T cells, and more DCs expressing MHC Ⅱ molecule. From the results, the redox-responsive polycation PAA as vaccine carriers helped elicit more potent cellular and humoral immune responses. Particularly, PAA induced much higher cellular immune response compared with previously reported carrier materials. The intelligent PAA could be developed as efficient mucosal vaccine delivery systems for clinical applications.

    更新日期:2017-09-07
  • Fibronectin in layer-by-layer assembled films switches tumor cells between 2D and 3D morphology
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-06
    Kiran Bhadriraju, Jennifer S. Hong, Steven P. Lund, Darwin R. Reyes

    Tumor cells showing a 3D morphology and in co-culture with endothelial cells are a valuable in vitro model for studying cell-cell interactions and for the development of pharmaceuticals. Here, we found that HepG2 cells, unlike endothelial cells, show differences in adhesion to fibronectin alone, or in combination with polyallylamine hydrochloride. This response allowed us to engineer micropatterned heterotypic cultures of the two cell types using a microfluidics to pattern cell adhesion. The resulting co-cultures exhibit spatially encoded and physiologically-relevant cell function. Further, we found that the protrusive, migratory and 3D morphological responses of HepG2 are synergistically modulated by the constituents of the hybrid extracellular matrix. Treating the hybrid material with the crosslinking enzyme transglutaminase inhibited the 3D morphogenesis of tumor cells. Our results extend previous work on the role of fibronectin in layer-by-layer assembled films, and demonstrate that cell-specific differences in adhesion to fibronectin can be used to engineer co-cultures.

    更新日期:2017-09-07
  • Interplay of Geometric Cues and RGD/BMP-2 Crosstalk in Directing Stem Cell Fate
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-05
    Ibrahim Bilem, Pascale Chevallier, Laurent Plawinski, Eli D. Sone, Marie-Christine Durrieu, Gaétan Laroche
    更新日期:2017-09-06
  • Mesenchymal stem cells engineering: microcapsules assisted gene transfection and magnetic cell separation
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-05
    Albert Muslimov, Alexander S. Timin, Aleksandra Petrova, Olga Epifanovskaya, Alena Shakirova, Kirill Lepik, Andrey Gorshkov, Eugenia Il’inskaja, Andrey Vasin, Boris Afanasyev, Boris Fehse, Gleb B. Sukhorukov

    Stem cell engineering, the manipulation and functionalization, involving genetic modification of cells can significantly expand their application for cell therapy in humans; however, reliable, standardized, and cost-effective methods for cell manipulation are required. Here we explored the potential of magnetic multilayer capsules to serve as universal platform for non-viral gene transfer, stem cell magnetization and magnetic cell separation to improve gene transfer efficiency. In particular the following experiments were performed: (i) the study of internalization process of magnetic capsules into stem cells, including capsule co-localization with established markers of endo-lysosomal pathway,(ii) characterization and quantification of capsule uptake with confocal, electron microscopy and flow cytometry, (iii) intracellular delivery of messenger RNA (mRNA) and separation of gene-introduced cells by MACS procedure, (iv) the influence of capsules on cell proliferation potential. Importantly, due to internalization of magnetic capsules by the cells, such cell engineering platform is responsive to external magnetic field, which allows to manipulate stem cells migration and perform separation of gene-introduced cells obtaining the population of almost entirely transfected cells (~ 95 %). These results suggest the multifunctional roles of magnetic capsules in stem cell functionalization for increasing gene transfer efficiency, separation of cells showing high capsule biocompatibility without loss of proliferative functions of stem cells.

    更新日期:2017-09-05
  • Tumor Neovasculature-Targeted APRPG-PEG-PDLLA/ MPEG-PDLLA Mixed Micelles loading Combretastatin A-4 for Breast Cancer Therapy
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-04
    Ying Hao, JinRong Peng, Yaguang Zhang, Lijuan Chen, Feng Luo, Cheng Wang, Zhiyong Qian

    Breast cancer has been the first killer among women. In this study, Combretastatin A-4 (CA-4) loaded 5-amino acid peptide Ala-Pro-Arg-Pro-Gly (APRPG) modified PEG-PDLLA mixed micelles was developed to target tumor neovasculature for breast cancer therapy. CA-4 is an effective vascular disrupting agent. The APRPG-modified PEG-PDLLA polymer was successfully synthesized and thin-film hydration method was used to prepare APRPG-PEG-PDLLA/MPEG-PDLLA mixed micelles. Drug loading capacity (DL), encapsulation efficiency (EE) and the optimized ratio of APRPG-PEG- PDLLA: MPEG-PDLLA for efficient drug loading was investigated. The particle size, zeta potential, morphology and the crystallographic study were carried out to characterize the micelles. In vitro release study revealed a sustained release of CA-4 from the mixed micelles while compared to free CA-4. Moreover, the cytotoxicity data of blank and drug loaded mixed micelles suggested that the APRPG-PEG-PDLLA/MPEG-PDLLA mixed micelles were safe drug carriers and the encapsulated CA-4 remained potent antitumor effect. The cellular uptake study and the in vivo imaging and biodistribution study demonstrated that the APRPG peptide modified mixed micelles has the higher cellular uptake efficiency and could significantly facilitate the accumulation at tumor site. Furthermore, the micelles were slowly extravasated from blood vessels and inhibited embryonic angiogenesis in the transgenic zebrafish model. Consequently, the CA-4 loaded APRPG-PEG-PDLLA/MPEG-PDLLA mixed micelles group demonstrated a significant inhibition of tumor growth in 4T1 breast cancer model. In short, the CA-4 loaded APRPG-PEG-PDLLA/MPEG-PDLLA mixed micelles might have great potential for breast cancer therapy.

    更新日期:2017-09-05
  • Collagen Fibril Response to Strain in Scaffolds from Ovine Forestomach for Tissue Engineering
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-05
    Katie H. Sizeland, Hannah C. Wells, Susyn J.R. Kelly, Keira E. Nesdale, Barnaby C. H. May, Sandi G. Dempsey, Christopher H. Miller, Nigel Kirby, Adrian Hawley, Stephen Mudie, Tim Ryan, David Cookson, Richard G. Haverkamp
    更新日期:2017-09-05
  • Characterization of Engineered Scaffolds with Spatial Prevascularized Networks for Bulk Tissue Regeneration
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-01
    Shuai Li, Hai-Guang Zhang, Dong-Dong Li, Jian-Ping Wu, Cheng-Yan Sun, Qing-Xi Hu
    更新日期:2017-09-04
  • Hyaluronic-Acid-Based pH-Sensitive Nanogels for Tumor-Targeted Drug Delivery
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-01
    Shujuan Luan, Yingchun Zhu, Xiaohe Wu, Yingying Wang, Fengguang Liang, Shiyong Song
    更新日期:2017-09-04
  • Rapid customization of 3D integrated microfluidic chips via modular structure-based design
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-02
    Jingjiang Qiu, Qing Gao, Haiming Zhao, Jianzhong Fu, Yong He

    In recent years, 3D integrated microfluidic systems have become increasingly more popular because of their ability to incorporate multifunctional components, including porous membranes and biological scaffolds. Due to limitations in resolution, fabrication efficiency and materials, it is hard to develop complex integrated microfluidic systems with low cost and high efficiency. In this paper, we present a novel method that utilizes modular structure-based design, which could greatly reduce the time and cost for customization of complete integrated chips, compared to traditional techniques. By printing sacrificial patterns on the substrate using the 3D printing approach and subsequently covering them with PDMS pre-polymer, PDMS slices with modular structures were obtained, each with specific functions. By combining different PDMS slices with specific modular structures and other functional components, such as membranes and scaffolds, the conceptual design was efficiently converted into complete integrated microfluidic chips. As proof-of-concept, customized 3D microfluidic chips were generated and successfully used for cell culture and biological analysis. Furthermore, the flexible combination with biofabrication of hydrogel beads was also presented, revealing the potential use of this technique in the fabrication of organ-on-a-chip.

    更新日期:2017-09-04
  • Single dose of protein vaccine with peptide nanofibers as adjuvants elicits long-lasting antibody titer
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-09-01
    Chengbiao Yang, Fang Shi, Can Li, Youzhi Wang, Ling Wang, Zhimou Yang

    Self-assembling materials based on peptides have shown great potential as vaccine adjuvants. In our previous work, we have demonstrated that nanofibers based on D-peptide Nap-GDFDFDY are good candidates for vaccine adjuvants. Here we further found that supramolecular hydrogels based on positively charged D-peptide Nap-GDFDFDYDK as vaccine adjuvants could induce stronger immune response. We designed and synthesized two D-peptide derivatives, one with a positive charge (Nap-GDFDFDYDK) and the other with a negative charge (Nap-GDFDFDYDE). Both of them could form the hydrogels constructed by nanofibers. The nanofibers formed by Nap-GDFDFDYDK promoted the more powerful immune response in mice against the antigen chicken egg albumin (OVA) than peptides Nap-GDFDFDY and Nap-GDFDFDYDE. Through cell experiments, we demonstrated that the main reason was that nanofibers formed by Nap-GDFDFDYDK could enhance the uptake of OVA by primary antigen presenting cells. Most importantly, it was intriguing that the nanofibers based on Nap-GDFDFDYDK could evoke long-lasting antibody titers for 28 weeks at a single dose of protein vaccine. Our study demonstrated that supramolecular hydrogels based on positively charged D-peptide were promising vaccine adjuvants and might be very useful for antibody production and vaccine development.

    更新日期:2017-09-04
  • Hierarchically Porous Calcium Carbonate Scaffolds for Bone Tissue Engineering
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-08-31
    Abiy Woldetsadik, Sudhir Sharma, Sachin Khapli, Ramesh Jagannathan, Mazin Magzoub

    Hierarchically porous CaCO3 scaffolds comprised of micron- (diameter = 2 ± 0.3 µm) and nano-sized (diameter = 50.42 ± 14.38 nm) pores were fabricated on silicon substrates using a supercritical CO2 process. Differentiated human THP-1 monocytes exposed to the CaCO3 scaffolds produced negligible levels of the inflammatory cytokine tumor necrosis factor-alpha (TNF-α), confirming the lack of immunogenicity of the scaffolds. Extracellular matrix (ECM) proteins, vitronectin (VN) and fibronectin (FN), displayed enhanced adsorption to the scaffolds compared to the silicon controls. ECM protein-coated CaCO3 scaffolds promoted adhesion, growth and proliferation of osteoblast MC3T3 cells. MC3T3 cells grown on the CaCO3 scaffolds produced substantially higher levels of transforming growth factor-beta (TGF-β) and vascular endothelial growth factor-A (VEGF-A), which regulate osteoblast differentiation, and exhibited markedly increased alkaline phosphatase (ALP) activity, a marker of early osteoblast differentiation, compared to controls. Moreover, the CaCO3 scaffolds stimulated matrix mineralization (calcium deposition), an endpoint of advanced osteoblast differentiation and an important biomarker for bone tissue formation. Taken together, these results demonstrate the significant potential of the hierarchically porous CaCO3 scaffolds for bone tissue engineering applications.

    更新日期:2017-09-04
  • A Bioinformatics 3D Cellular Morphotyping Strategy for Assessing Biomaterial Scaffold Niches
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-08-31
    Stephen Florczyk, Mylene Simon, Derek Juba, Scott Pine, Sumona Sarkar, Desu Chen, Paula Baker, Subhadip Bodhak, Antonio Cardone, Mary Brady, Peter Bajcsy, Carl G Simon

    Many biomaterial scaffolds have been advanced to provide synthetic cell niches for tissue engineering and drug screening applications, however current methods for comparing scaffold niches focus on cell functional outcomes or attempt to normalize materials properties between different scaffold formats. We demonstrate a three-dimensional (3D) cellular morphotyping strategy for comparing biomaterial scaffold cell niches between different biomaterial scaffold formats. Primary human bone marrow stromal cells (hBMSCs) were cultured on 8 different biomaterial scaffolds, including fibrous scaffolds, hydrogels, and porous sponges, in 10 treatment groups to compare a variety of biomaterial scaffolds and cell morphologies. A bioinformatics approach was used to determine the 3D cellular morphotype for each treatment group by using 82 shape metrics to analyze approximately 1000 cells. We found that hBMSCs cultured on planar substrates yielded planar cell morphotypes, while those cultured in 3D scaffolds had elongated or equiaxial cellular morphotypes with greater height. Multivariate analysis was effective at distinguishing mean shapes of cells in flat substrates from cells in scaffolds, as was the metric L1-depth (the cell height along its shortest axis after aligning cells with a characteristic ellipsoid). The 3D cellular morphotyping technique enables direct comparison of cellular microenvironments between widely different types of scaffolds and design of scaffolds based on cell structure-function relationships.

    更新日期:2017-08-31
  • Collagen Fibril Intermolecular Spacing Changes with 2-Propanol: A Mechanism for Tissue Stiffness
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-08-30
    Hannah C. Wells, Katie H. Sizeland, Susyn Kelly, Nigel Kirby, Adrian M. Hawley, Stephen Mudie, Richard G. Haverkamp

    Materials composed primarily of collagen are important as surgical scaffolds and other medical devices and require flexibility. However, the factors that control the suppleness and flexibility of these materials are not well understood. Acellular dermal matrix materials in aqueous mixtures of 2-propanol were studied. Synchrotron based small angle X-ray scattering was used to characterize the collagen structure and structural arrangement. Stiffness was measured by bend tests. Bend modulus increased logarithmically with 2-propanol concentration from 0.5 kPa in water to 103 kPa in pure 2-propanol. The intermolecular spacing between tropocollagen molecules decreased from 15.3 to 11.4 Å with increasing 2-propanol concentration while fibril diameter decreased from 57.2 to 37.2 nm. D-spacing initially increased from 63.6 nm to 64.2 nm at 50% 2-propanol then decreased to 60.3 nm in pure 2-propanol. The decrease in intermolecular spacing and fibril diameter are due to removal of water and the collapse of the hydrogen bond structure between tropocollagen molecules causing closer packing of the molecules within a fibril. We speculate this tighter molecular packing may restrict the sliding of collagen within fibrils, and similar disruption of the extended hydration layer between fibrils may lead to restriction of sliding between fibrils. This mechanism for tissue stiffness may be more general.

    更新日期:2017-08-31
  • Silver nanowire particle reactivity with human monocyte-derived macrophage cells: intracellular availability of silver governs their cytotoxicity
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-08-30
    Ioannis G. Theodorou, Karin H. Müller, Shu Chen, Angela E. Goode, Vladimir Yufit, Mary P. Ryan, Alexandra E Porter

    Silver nanowires (AgNWs) are increasingly being used in the production of optoelectronic devices, with manufacturing processes posing a risk for occupational exposures via inhalation. Although some studies have explored the environmental effects of AgNWs, few data exist on human health effects. Alveolar macrophages are central in the clearance of inhaled fibers from the lungs, with frustrated phagocytosis often stated as a key determinant for the onset of inflammatory reactions. However, the mechanisms through which fully ingested AgNWs interact with, degrade and transform within primary macrophages over time, and whether the reactivity of the AgNWs arises due to ionic or particulate effects, or both, is poorly understood. Here, a combination of elemental quantification, 3D tomography, analytical transmission electron microscopy (TEM), and confocal microscopy were employed to monitor the uptake, intracellular Ag+ availability and processing of AgNWs of two different lengths (1 and 10 μm) inside human monocyte-derived macrophages (HMMs). Using AgNO3 and spherical silver nanoparticles (AgNPs) as a comparison, the amount of total bioavailable/intracellular Ag highly correlated to the cytotoxicity of AgNWs. The 10 μm AgNWs were completely internalized in HMMs, with numerous lysosomal vesicles observed in close vicinity to the AgNWs. Following cellular uptake, AgNWs dissolved and transformed intracellularly, with precipitation of AgCl as well as Ag2S. These transformation processes were likely due to AgNW degradation in the acidic environment of lysosomes, leading to the release of Ag+ ions that rapidly react with Cl- and SH- species of the cell microenvironment. Our data suggest that in HMMs, not only frustrated phagocytosis, but also the extent of intracellular uptake and dissolution of AgNWs dictates their cytotoxicity.

    更新日期:2017-08-31
  • Localized Immunomodulatory Silk Macrocapsules for Islet-like Spheroid Formation and Sustained Insulin Production
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-08-30
    Manishekhar Kumar, Samit K. Nandi, David L. Kaplan, Biman B. Mandal
    更新日期:2017-08-31
  • Oxygen-Releasing Coatings for Improved Tissue Preservation
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-08-30
    Aurelien Forget, Camille Staehly, Neethu Ninan, Frances J. Harding, Krasimir Vasilev, Nicolas H. Voelcker, Anton Blencowe
    更新日期:2017-08-31
  • Bactericidal Peptidomimetic Polyurethanes with Remarkable Selectivity against Escherichia coli
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-08-30
    Steven Mankoci, Ricky L. Kaiser, Nita Sahai, Hazel A. Barton, Abraham Joy
    更新日期:2017-08-31
  • Structural Analysis and Protein Functionalization of Electroconductive Polypyrrole Films Modified by Plasma Immersion Ion Implantation
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-08-30
    Alexey Kondyurin, Kostadinos Tsoutas, Quentin-Xavier Latour, Michael J. Higgins, Simon E. Moulton, David R. McKenzie, Marcela M. M. Bilek
    更新日期:2017-08-31
  • Biodegradable and Magnetic-Fluorescent Porous Silicon@Iron Oxide Nanocomposites for Fluorescence/Magnetic Resonance Bimodal Imaging of Tumor in Vivo
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-08-30
    Bing Xia, Jiachen Li, Jisen Shi, Yu Zhang, Qi Zhang, Zhenyu Chen, Bin Wang
    更新日期:2017-08-31
  • Improved Antithrombotic Function of Oriented Endothelial Cell Monolayer on Microgrooves
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-08-30
    Jia-yan Chen, Mi Hu, He Zhang, Bo-chao Li, Hao Chang, Ke-feng Ren, Yun-bing Wang, Jian Ji
    更新日期:2017-08-31
  • Developing Hybrid Polymer Scaffolds Using Peptide Modified Biopolymers for Cell Implantation
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-08-30
    Sinoj Abraham, Purushothaman Kuppan, Shammy Raj, Bassem Salama, Gregory S. Korbutt, Carlo D. Montemagno
    更新日期:2017-08-30
  • Permeabilization-Tolerant Plasma Membrane Imaging Reagent Based on Amine-Rich Glycol Chitosan Derivatives
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-08-30
    Hong-Yin Wang, Jie Sun, Liu-Yuan Xia, Yan-Hong Li, Zhan Chen, Fu-Gen Wu
    更新日期:2017-08-30
Some contents have been Reproduced with permission of the American Chemical Society.
Some contents have been Reproduced by permission of The Royal Society of Chemistry.
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