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Simultaneous quantification and confirmation of oxycodone and its metabolites in equine urine using ultra-high performance liquid chromatography-tandem mass spectrometry J. Chromatogr. B (IF 3.0) Pub Date : 2024-04-12 Youwen You, Jaclyn R. Missanelli, Rachel M. Proctor, Joanne Haughan, Mary A. Robinson
Oxycodone, an opioid commonly used to treat pain in humans, has the potential to be abused in racehorses to enhance their performance. To understand the pharmacokinetics of oxycodone and its metabolites in horses, as well as to detect the illegal use of oxycodone in racehorses, a method for quantification and confirmation of oxycodone and its metabolites is needed. In this study, we developed and validated
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Derivatization-free determination of chiral plasma pharmacokinetics of MDMA and its enantiomers J. Chromatogr. B (IF 3.0) Pub Date : 2024-04-09 Dino Luethi, Deborah Rudin, Isabelle Straumann, Jan Thomann, Isidora Avedisian, Matthias E. Liechti, Urs Duthaler
3,4-Methylenedioxymethamphetamine (MDMA) is an entactogen with therapeutic potential. The two enantiomers of MDMA differ regarding their pharmacokinetics and pharmacodynamics but the chiral pharmacology of MDMA needs further study in clinical trials. Here, an achiral and an enantioselective high performance liquid chromatography–tandem mass spectrometry method for the quantification of MDMA and its
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A magnetic calcium phosphate for selective capture of multi-phosphopeptides J. Chromatogr. B (IF 3.0) Pub Date : 2024-04-06 FangKun Ren, JunYong Dai, JingYi Zhang, YanFei Luan, Fan Yang, Jian Shen, HaiLong Liu, JiaHong Zhou
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Quantification of the aromatase inhibitor letrozole and its carbinol metabolite in mouse plasma by UHPLC-MS/MS J. Chromatogr. B (IF 3.0) Pub Date : 2024-04-02 Hanieh Taheri, Yan Jin, Eman Ahmed, Peng Hu, Yang Li, Alex Sparreboom, Shuiying Hu
A liquid chromatography - electrospray ionization-mass spectrometry (LC-ESI-MS) method was developed for the quantification of letrozole, a third-generation aromatase inhibitor, and its main carbinol metabolite (CM) in support of murine pharmacokinetic studies. Using polarity switching, simultaneous ESI-MS measurement of letrozole and CM was achieved in positive and negative mode, respectively. The
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On-site monitoring of nandrolone in cattle farming samples by portable atmospheric pressure chemical ionization mass spectrometry with ambient sampling J. Chromatogr. B (IF 3.0) Pub Date : 2024-04-02 Jiahui Wu, Qingcheng Wang, Oushan Tang, Jie Liao, Qiaoling Zhao, Jingjing Liang, Huina Feng, Linhua Wang, Haixing Wang, Qing Shen
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A new surface plasmon resonance-based immunoassay for rapid and sensitive quantification of D-dimer in human plasma for thrombus screening J. Chromatogr. B (IF 3.0) Pub Date : 2024-04-02 Xin'er Hu, Diya Lv, Minyu Qi, Ying Zhang, Xiaofei Wang, Jiayu Gu, Dongyao Wang, Xiaofei Chen, Yue Liu, Yan Cao, Hai Zhang
D-dimer is a protein fragment generated during the fibrin breakdown by plasmin, and it serves as a mature biomarker for diagnosing thrombotic disorders. A novel immunoassay method based on surface plasmon resonance (SPR) has been developed, validated, and successfully applied for the quantification of D-dimer in human plasma with high sensitivity and rapidity. In this methodological study, we investigated
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Isolation and purification of high molecular weight adiponectin from human plasma fraction J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-30 Kyung Hyun Boo, Jin Woo Kim, Minkyung Song
Adiponectin, a crucial protein hormone originating from adipose tissue, regulates key metabolic processes, including lipid metabolism, mitochondrial activity, and insulin sensitivity. These pleiotropic roles of adiponectin, along with its inverse correlation with metabolic disorders such as obesity, type II diabetes, and atherosclerosis, establish this protein as a potential therapeutic target. However
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Development of dispersive solid phase extraction based on magnetic metal organic framework for the extraction of sunitinib in biological samples and its determination by high performance liquid chromatography-tandem mass spectrometry J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-29 Mandana Hassani Nouriyeh, Mohammad Reza Afshar Mogaddam, Mahboob Nemati, Mir Ali Farajzadeh, Aysa Abbasalizadeh, Ali Shahedi Hojghan
Herein, a simple, sensitive, and reliable dispersive solid phase extraction was reported for the efficient extraction of sunitinib from biological samples. To facilitate the extraction of the desired analyte from urine and plasma samples, magnetic MIL-101Cr (NH) @SiO @ NiFeO was synthesized by a hydrothermal method and applied as an effective sorbent during the extraction process. After adsorption
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Development of a new continuous homogenous liquid phase microextraction procedure based on in-situ preparation of deep eutectic solvent; application in the analysis of aliphatic amines in urine samples by GC–MS J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-29 Shabnam Shagaghipour, Mohammad Reza Afshar Mogaddam, Saeed Mohammad Sorouraddin, Mir Ali Farajzadeh
In the present work, a new microextraction procedure combined with gas chromatography-mass spectrometry has been developed for the analysis of several aliphatic amines from urine sample. The sample preparation method was a continuous homogenous liquid phase microextraction that was based on in-situ preparation of 4-chlorophenol: choline chloride deep eutectic solvent. The deep eutectic solvent was
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Stability study and validation of a liquid chromatography tandem mass spectrometry method for the quantitative analysis of polyphenols in fish feed ingredients J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-28 Viviana Sarmiento, Kristin Hamre, Aleksander Arnø, Joshua Dagogo, Elisabeth Ødegård, Odd Elvebø, Pedro Araujo
The perception of polyphenols as a safe, healthy, and sustainable solution for replacing synthetic antioxidants has been an important factor for their rapid growing in the global food market. Therefore, it is essential to use reliable methods for their quantification in commercial products intended for animal or human consumption. The purpose of this study is to evaluate the performance of some solvents
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Analysis of alterations in serum vitamins and correlations with gut microbiome, microbial metabolomics in patients with sepsis J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-26 Yingchen Wang, Susu Feng, Hongwei Shi, Yuxin Lu, Jingtao Zhang, Wanglin Zhang, Yuzhi Xu, Qi Liang, Liqun Sun
Vitamins are essential micronutrients that play key roles in many biological pathways associated with sepsis. The gut microbiome plays a pivotal role in the progression of sepsis and may contribute to the onset of multi-organ dysfunction syndrome (MODS). The aim of this study was to investigate the changes in serum vitamins, and their correlation with intestinal flora and metabolomic profiles in patients
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Simultaneous determination of 11 oral targeted antineoplastic drugs and 2 active metabolites by LC-MS/MS in human plasma and its application to therapeutic drug monitoring in cancer patients J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-26 Jing Zhao, Dongming Yan, Yue Li, Xiaoqing Xu, Fengling Li, Shuang Zhang, Jingyi Jin, Furong Qiu
Interindividual exposure differences have been identified in oral targeted antineoplastic drugs (OADs) owing to the pharmacogenetic background of the patients and their susceptibility to multiple factors, resulting in insufficient efficacy or adverse effects. Therapeutic drug monitoring (TDM) can prevent sub-optimal concentrations of OADs and improve their clinical treatment. This study aimed to develop
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The potential of polyaniline-coated stationary phase in hydrophilic interaction liquid chromatography-based solid-phase extraction for glycopeptide enrichment J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-26 Katarina Molnarova, Tomas Krizek, Petr Kozlik
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LC-MS/MS methods for simultaneous determination of youkenafil and its metabolite M1 in human seminal plasma and plasma: Application to evaluate the acute effect of youkenafil on semen quality and its pharmacokinetics in human J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-25 Keli Wang, Xinrui Zhang, Fengqin Chen, Yufeng Ni, Zhengwen Cai, Juefang Ding, Shaochun Chen, Chang Shu, Li Ding
Youkenafil is a novel Phosphodiesterase type 5 inhibitor used for treating erectile dysfunction. N-desethyl compound of youkenafil (M1) is its main active metabolite. In this study, two methods were developed and validated for the simultaneous determination of youkenafil and M1 by HPLC-MS/MS in human matrices including seminal plasma and plasma, in which the multiple reaction monitoring and electrospray
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Development and validation of an LC-MS/MS method for quantifying diamorphine and its major metabolites 6-monoacetylmorphine, morphine, morphine-3-glucuronide, and morphine-6-glucuronide in human plasma J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-25 Jan Thomann, Severin B. Vogt, Adrian Guessoum, Maximilian Meyer, Marc Vogel, Matthias E. Liechti, Dino Luethi, Urs Duthaler
Diamorphine, commonly known as heroin, is a semi-synthetic opioid analgesic. In the context of heroin-assisted treatment for opioid-dependent patients, diamorphine is mostly administered intravenously. However, recent attention has shifted towards intranasal administration as a better-tolerated alternative to the intravenous route. Here, we developed and validated a rapid bioanalytical method for the
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A high-throughput method for the determination of 14 UV-filters in human plasma by LC-MS/MS: Minimize interferences from proteins and phospholipids in the matrix J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-20 Wucheng Yang, Jianglu Feng, Wenyao Liang, Mingxia Nie, Jianhua Tan, Ruifang Fan
Accurate monitoring of UV-filters exposure levels in human plasma is a challenge because of the significant differences in the physicochemical properties of UV-filters, as well as the matrix effect caused by abundant proteins and phospholipids in plasma. Therefore, an effective and rapid method for simultaneous determination of 14 UV-filters in human plasma using protein precipitation-solid phase extraction
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Simultaneous mass spectrometric quantification of trace amines, their precursors and metabolites J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-18 Krisztina de Bruyn, Eugene F. Diekman, Claude P. van der Ley, Martijn van Faassen, Ido P. Kema
Trace amines are powerful neuromodulators influencing the release and reuptake of catecholamines. These low concentrated endogenous amines impact mood, cognition, and hormone regulation. Dysregulation of trace amines have been associated with a variety of diseases, such as schizophrenia, Parkinson’s disease, migraine, depression and more. Succesfull simultaneous quantification of trace amines, their
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Identification and quantification of chain-pairing variants or mispaired species of asymmetric monovalent bispecific IgG1 monoclonal antibody format using reverse-phase polyphenyl chromatography coupled electrospray ionization mass spectrometry J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-16 Ryte Poskute, Praveen Kallamvalliillam Sankaran, Laura Sewell, Giordana Lepore, Rebecca Shrubsall, Lydia Dewis, Yasunori Watanabe, Vanessa Wong, Laura Pascual Fernandez, Rahul Mishra, Alexander Holt, Susie Sou, Claire Harris, Cristina Moreno Rodriguez, Ayca Cankorur-Cetinkaya, Jennifer Smith, Nikola Lonska, Adam Powell, Tingting Cui, Matthew Cheeks, Viv Lindo
Developing a knob-into-hole asymmetric bispecific IgG1 monoclonal antibody (mAb) poses manufacturing challenges due to the expression of chain pairing variants, also called mispaired species, in the desired product. The incorrect pairing of light and heavy chains could result in heterogeneous mispaired species of homodimers, heterodimers, light chain swapping, and low molecular weight species (LMWS)
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Simultaneous determination of twelve neonicotinoids and six metabolites in human urine with isotope-dilution UPLC-Q-Orbitrap HRMS J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-14 Boya Zhang, Zheng Wang, Jiming Zhang, Yiming Dai, Jiayun Ding, Jianqiu Guo, Yuhang Chen, Dasheng Lu, Chunhua Wu, Zhijun Zhou
The extensive global use of neonicotinoid insecticides (NNIs) has led to widespread human exposure, necessitating the development of effective methods for large-scale biomonitoring. However, current methods are inadequate in simultaneously and accurately detecting various NNIs or their metabolites (m-NNIs). In this study, we aimed to establish a robust method using solid-phase extraction (SPE)-ultra
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Development of a hollow fiber–liquid phase microextraction method using tissue culture oil for the extraction of free metoprolol from plasma samples J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-13 Ali Akbar Fathi, Mir Ali Farajzadeh, Ali Shahedi, Mohammad Reza Afshar Mogaddam, Jalil Houshyar, Abolghasem Jouyban
In this research, a method known as a hollow fiber-liquid-phase microextraction was employed to extract and concentrate free metoprolol from plasma samples. The extracted analyte was subsequently determined using high-performance liquid chromatography coupled with a diode-array detector. Several parameters, including hollow fiber length, sonication time, extraction temperature, and salt addition, were
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Analysis of vitamin D and its metabolites in biological samples – Part I: Optimization and comparison of UHPSFC-MS/MS and UHPLC-MS/MS methods J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-12 Veronika Pilařová, Bárbara Socas-Rodríguez, Lucie Nováková, Sofia Essén, Cecilia Holm, Charlotta Turner, Margareta Sandahl
Fat-soluble vitamin D is an essential bioactive compound important for human health. Insufficient vitamin D levels can result not only in bone disease but also in other disorders, such as cancer, metabolic disorders, and diseases related to poor immune function. The current methods commonly used for vitamin D analysis are often applied to determine the levels of the most abundant metabolite in plasma
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Analysis of vitamin D and its metabolites in biological samples – Part II: Optimization of a sample preparation method for liver tissue J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-11 Veronika Pilařová, Bárbara Socas-Rodríguez, Lucie Nováková, Cecilia Holm, Margareta Sandahl, Charlotta Turner
Extraction of vitamin D, including its hydroxylated and esterified metabolites, from soft tissues such as the liver is challenging due to the lipophilic character of matrix and analytes that are expected in very low concentration levels.
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Exploring the potential of using ion mobility-mass spectrometry to separate matrix interferences from analytes in food control J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-11 Sjors Rasker, Marco H. Blokland, Toine F.H. Bovee, Ane Arrizabalaga-Larrañaga
During residue analysis in complex matrices for food safety purposes, interfering signals can sometimes overlap with those of the analyte of interest. Access to an additional separation dimension besides chromatographic and mass separation, such as ion mobility, can aid in removing interfering signals, allowing for correct analyte identification in these cases. In our laboratory, during routine LC−MS/MS
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Sensitive, reliable and simultaneous determination of Fingolimod and Citalopram drug molecules used in multiple sclerosis treatment based on magnetic solid phase extraction and HPLC-PDA J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-11 Esra Durgun, Halil İbrahim Ulusoy, İbrahim Narin
An analytical methodology has been developed for trace amounts of Fingolimod (FIN) and Citalopram (CIT) drug molecules based on magnetic solid phase extraction (MSPE) and high performance liquid chromatographic determination with photodiode array detector (HPLC-DAD). Fingolimod is used in treatment of Multiple sclerosis (MS) disease and sometimes antidepressant drugs such as citalopram accompany to
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Pro-inflammatory enzyme inhibition of lipoxygenases by flavonoid rich extract from Artemisia vulgaris J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-10 Jin Ru Lim, Lee Suan Chua, Azizul Azri Mustaffa
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Generation of affinity maps for thiazolidinediones with human serum albumin using affinity microcolumns. I. Studies of effects by glycation on multisite drug binding J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-03 Sadia Sharmeen, Ashley Woolfork, David S. Hage
Human serum albumin (HSA) is known to undergo modifications by glucose during diabetes. This process produces glycated HSA that can have altered binding to some drugs. In this study, high-performance affinity microcolumns and competition studies were used to see how glycation affects the binding by two thiazolidinedione-class drugs (i.e., pioglitazone and rosiglitazone) at specific regions of HSA.
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Composition and nutritional values of fatty acids in marine organisms by one-step microwave-assisted extraction/derivatization and comprehensive two-dimensional gas chromatography -flame ionization detector J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-02 Donatella Ferrara, Mirco Cescon, Giulia Giacoppo, Valentina Costa, Giorgia Purcaro, Natasha Damiana Spadafora, Chiara Cordero, Luisa Pasti, Alberto Cavazzini, Marco Beccaria
This work reports the characterization of the lipidic fraction of seven species of marine organisms gathered along the shoreline of the Po Delta Park of Emilia-Romagna Region (Italy) and of the north Adriatic Sea. Two species of oysters ( and ), two species of clams ( and ), one species of mussel (), one species of macroalgae (), and one species of spiny dogfish () were analyzed to characterize their
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New azaspiracid analogues detected as bi-charged ions in Azadinium poporum (Amphidomataceae, Dinophyceae) isolated from Japanese coastal waters J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-02 Mayu Ozawa, Hajime Uchida, Ryuichi Watanabe, Satoshi Numano, Ryoji Matsushima, Hiroshi Oikawa, Kazuya Takahashi, Wai Mun Lum, Garry Benico, Mitsunori Iwataki, Toshiyuki Suzuki
Lipophilic marine biotoxin azaspiracids (AZAs) are produced by dinoflagellates and . Recently, several strains of were isolated from Japanese coastal waters, and detailed toxin profiles of two strains (mdd421 and HM536) among them were clarified by several detection techniques on liquid chromatography-tandem mass spectrometry (LC-MS/MS) and liquid chromatography-quadrupole time of flight mass spectrometry
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Validation of a human saliva model for the determination of leachable monomers and other chemicals from dental materials J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-01 Lene A. Grutle, Heidi V. Holm, Hilde B.M. Kopperud, Silvio Uhlig
This study aimed to prove the validity of a mixture of chemicals, including salts, small organic molecules, mucin, and α-amylase, as saliva surrogate (“artificial saliva”) for assessing leakage of methacrylate monomers and other constituents from dental materials. To achieve this, we developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of
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Study on material basis and anti-hypertensive metabolomics of Zhengan-Xifeng-Tang(ZXT): A comparison between ZXT decoction and granules J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-01 Haichao Li, Lihua Wang, Hao Zhang, Wenchi Yu, Yunlun Li, Haiqing Jiang, Danyang Wang, Yu Wang
High blood pressure is a serious human health problem and one of the leading risk factors for fatal complications in cardiovascular disease. The ZXT granules were prepared based on the Zhengan-Xifeng-Tang (ZXT) decoction. However, the therapeutic effects of ZXT granules on spontaneous hypertension and the metabolic pathways in which they may intervene are unclear. The aim of this study was to investigate
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Effective separation of maslinic acid and oleanolic acid from olive pomace using high-speed shear off-line coupled with high-speed countercurrent chromatography and their antibacterial activity test J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-01 Kun Yang, Sheng-Bing Wang, Dong Pei, Lu-Mei Pu, Xin-Yi Huang
In this work, a high-speed shear extraction off-line coupling high-speed countercurrent chromatography method was developed to separate maslinic acid and oleanolic acid from olive pomace. To improve extraction efficiency, the polar disparity between maslinic acid and oleanolic acid necessitated the concurrent utilization of both polar and non-polar solvents during high-speed shear extraction. Then
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A quantitative LC-MS/MS analysis of Xylazine, p-Fluorofentanyl, Fentanyl and Fentanyl-Related compounds in postmortem blood J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-28 Michael T. Truver, Sara C. Brogan, Emma A. Jaeschke, Amy M. Kinsey, Jennifer L. Hoyer, Chris W. Chronister, Michele M. Crosby, Bruce A. Goldberger
The purpose of this study was to develop and validate a method to quantitate the veterinary sedative xylazine as well as 4-anilino-N-phenethylpiperidine (4-ANPP), acetyl fentanyl, fentanyl, norfentanyl, and fluorofentanyl in blood utilizing liquid chromatography tandem mass spectrometry. This method also qualitatively monitors for the presence of -fluorofentanyl and -fluorofentanyl isomers. UCT Clean
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Rapid evaluation of PHD2 inhibitory activity of natural products based on capillary electrophoresis online stacking strategy J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-28 Ying Zhang, Chun-Yan Sang, Xing-Rong Wang, Cheng-Bo Wang, Xian-Hua Meng, Wei-Feng Wang, Jun-Li Yang
Prolyl hydroxylase domain 2 (PHD2) is an important enzyme in the human body that perceives changes in oxygen concentration and regulates response in hypoxic environments. Evaluation of PHD2 inhibitory activity of natural products is crucial for drug development of hypoxia related diseases. At present, the detection of low concentration of -ketoglutaric acid (the substrate of PHD2 enzymatic reaction)
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Development and validation of LC-MS/MS for quantifying omadacycline from stool for gut microbiome studies J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-28 Chenlin Hu, Weiqun Wang, Jinhee Jo, Kevin W. Garey
This study developed and validated a new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify omadacycline and its epimerization in stool to facilitate microbiome studies. Omadacycline was extracted in a methanol-water-ethylenediaminetetraacetic acid (ETDA) solvent containing deuterated omadacycline as internal standard, followed by dilution. In an optimal gradient elution mode
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A simple tandem bioassay-guided SCX-RP SPE fractionation for efficient active peptide screening from Inca nut cake protein hydrolysate J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-28 Christoper Caesar Yudho Sutopo, Wei-Ting Hung, Jue-Liang Hsu
Typically, bioactive peptides were uncovered from complex hydrolysates using sequential bioassay-guided fractionation. To increase the efficiency of bioactive peptide screening, a simple and convenient tandem bioassay-guided fractionation based on solid-phase extraction (SPE) was conducted to screen the angiotensin-I-converting enzyme (ACE) inhibitory peptides from the hydrolysate of Inca nut cake
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Accurate redox state indication by in situ derivatization with N-ethylmaleimide - Profiling of transsulfuration and glutathione pathway metabolites by UPLC-MS/MS J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-27 Mathias Langner, Dennis Fröbel, Jana Helm, Triantafyllos Chavakis, Mirko Peitzsch, Nicole Bechmann
Reduced and oxidized glutathione play an important role for the intracellular detoxification of reactive oxygen species. The iron-dependent formation of such reactive oxygen species in conjunction with the inhibition of the redox-balancing enzyme glutathione peroxidase 4 underlie an imbalance in the cellular redox state, thereby resulting in a non-apoptotic form of cell death, defined as ferroptosis
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Updates of the current strategies of labeling for N-glycan analysis J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-27 Yosra Helali, Cédric Delporte
This mini review summarizes the current methods used for screening glycosylation of glycoproteins, with a specific focus on therapeutic proteins and on techniques involving the release of glycans. With the continuous development of biopharmaceuticals, particularly monoclonal antibodies (mAbs), which are glycosylated proteins, monitoring has gained importance in recent decades. Glycosylation of therapeutic
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Utilization of a pH-switchable hydrophilicity solvent for the microextraction of clomipramine from human urine samples J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-23 Maria Terzi, Natalia Manousi, Paraskevas D. Tzanavaras, Constantinos K. Zacharis
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A simple UHPLC-MS/MS method for determination of SET2, a selective antagonist of TRPV2 receptor, in rat plasma samples J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-23 Linda Bartosova, Peter Balis, Vladimir Garaj, Andrej Kovac, Tomas Rajtik, Juraj Piestansky
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Molecular size distribution in pentavalent (A, C, Y, W, X) meningococcal polysaccharide conjugate vaccine by HPSEC-UV-MALS-RI method- a conceivable stability indicating parameter J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-22 Pankaj Sharma, Sameer Kale, Swapnil Phugare, Sumeet Pendharkar, Prasad Shinde, Aarushi Mandhan, Sunil Kumar Goel, Sunil Gairola
Molecular size distribution (MSD) of polysaccharides serves as a key parameter that directly correlates to the immunogenicity of vaccine. MSD at meningococcal polysaccharide (A, C, Y and W) or conjugate bulk level is well established under detailed pharmacopeial and WHO guidelines. We report here, a newly developed method for determination of molecular size distribution of pentavalent Meningococcal
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Corrigendum to “Development and validation of a gradient HPLC-UV method for mitragynine following in vitro skin permeation studies” [J. Chromatogr. B 1204 (2022) 123316] J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-21 Yee Shan Sim, Zan Yang Chong, Juzaili Azizi, Choon Fu Goh
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A new combined technology: Macroporous adsorption resin and high speed counter current chromatography for hydroxytyrosol separation from olive leaf enzymatic hydrolysate J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-17 Jiaojiao Yuan, Su Tuo, Xiuhong Shi, Junling Tu
Due to three free hydroxyl groups, hydroxytyrosol (HT) presents strong bioactivity and has broad food and drug application prospects. However, there is no good separation and purification technology. In this study, separation and purification technology of HT from the ethyl acetate extraction of enzymatic hydrolysate from olive leaf (EEEH) was investigated with macroporous adsorption resin (MAR) and
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Measurement of short-chain fatty acids in human plasma by means of fast gas chromatography-mass spectrometry J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-16 Giuseppe Micalizzi, Chiara Buzzanca, Valentina Chiaia, Monica Mondello, Francesco Cacciola, Daniela Caccamo, Luigi Mondello
A rapid and practicable analytical method for the measurement of short-chain fatty acids (SCFAs) in human plasma was developed. The extraction procedure involved the use of acidified water and methyl -butyl ether (MTBE), while the separation and detection of SCFAs, including acetic, propionic, and butyric acids was carried out by using gas chromatography-mass spectrometry (GC–MS) technique. The novelty
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Optimizing and integrating depletion and precipitation methods for plasma proteomics through data-independent acquisition-mass spectrometry J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-16 Yue Zhou, Helong Zheng, Zengqi Tan, Enci Kang, Peng Xue, Xiang Li, Feng Guan
The application of plasma proteomics is a reliable approach for the discovery of biomarkers. However, the utilization of mass spectrometry-based proteomics in plasma encounters limitations due to the presence of high-abundant proteins (HAPs) and the vast dynamic range. To address this issue, we conducted an optimization and integration of depletion and precipitation strategies eliminating interference
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Urinary excretion profile of higenamine in females after oral administration of supplements – Doping scenario J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-15 B. Stojanovic, J. Rasic, M. Andjelkovic, N. Dikic, N. Dragicevic, B. Djordjevic, G. Forsdahl, G. Gmeiner
In 2017, higenamine was added to the (WADA) Prohibited list under group S3: beta-2 agonists and it is banned for athletes both in – and out of competition. Aim of this study was to characterize the urinary excretion profile of higenamine and its metabolite coclaurine after oral administration of multiple doses of higenamine capsules. For this purpose, an administration study including female basketball
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Simultaneous determination of HD56, a novel prodrug, and its active metabolite in cynomolgus monkey plasma using LC-MS/MS for elucidating its pharmacokinetic profile J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-13 Shi Yao, Wenpeng Zhang, Junhai Xiao, Zhiwei Zhang, Lingchao Wang, Hengxiao Ai, Xia Wu, Aibing Chen, Xiaomei Zhuang
An LC-MS/MS method was developed and validated for the simultaneous determination of the carboxylic acid ester precursor HD56 and the active product HD561 in cynomolgus monkey plasma. Then, the pharmacokinetic characteristics of both compounds following single and multiple administrations in cynomolgus monkeys were elucidated. In the method, chromatographic separation was achieved with a C18 reversed-phase
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A validated LC-MS/MS method for determination of six Anti-SARS-CoV-2 drugs in plasma and its application for a pharmacokinetic study in rats J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-07 Zong-liang Xu, Cheng-jian Li, Xian Qian, Hu Duan, Jin Zhou, Qian-qian Zhang, Xin Dong, Liang Zhao
Antiviral treatment for COVID-19 is considered an effective tool in reducing the rate of severe cases and deaths. As of June 2023, a total of six small molecule antiviral drugs have been conditionally approved for marketing by the National Medical Products Administration (NMPA) within China. In this study, a method of HPLC-MS/MS was established and validated for the determination of six small molecule
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Simultaneous quantification of TB-500 and its metabolites in in-vitro experiments and rats by UHPLC-Q-Exactive orbitrap MS/MS and their screening by wound healing activities in-vitro J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-07 Khandoker Asiqur Rahaman, Anca Raluca Muresan, Hophil Min, Junghyun Son, Hyung-Seop Han, Min-Jung Kang, Oh-Seung Kwon
TB-500 (Ac-LKKTETQ), derived from the active site of thymosin β4 (Tβ4), has various biological functions in its unacetylated form, LKKTETQ. These functions include actin binding, dermal wound healing, angiogenesis, and skin repair. The biological effects of TB-500, however, have not been documented. And the analysis of TB-500 and its metabolites have been neither simultaneously quantified nor structurally
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An innovative immunochromatographic assay employing Pt-Pd bimetallic nanoparticles as labels for the detection of foot-and-mouth disease virus serotype O J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-07 Yanyan Sun, Xin Li, Mi Lin, Guang Yang, Huali He, Yanfang Bao, Fengsong Li, Tao Jiang
We created a novel, high sensitivity immunochromatographic assay that allows for clear and precise quantitative analysis by employing innovative bimetallic nanoparticles with peroxide-like activity as markers for the preparation of the test strip. Initially, we synthesized Pt-Pd bimetallic nanoparticles through the reduction of KPtCl and NaPdCl using ascorbic acid (AA) in an ultrasonic water bath.
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Optimization and validation of a UPLC-MS/MS assay for simultaneous quantification of 2,8-dihydroxyadenine, adenine, allopurinol, oxypurinol and febuxostat in human plasma J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-05 Unnur A. Thorsteinsdottir, Hrafnhildur L. Runolfsdottir, Finnur F. Eiriksson, Inger M. Sch. Agustsdottir, Vidar O. Edvardsson, Runolfur Palsson, Margret Thorsteinsdottir
Adenine phosphoribosyltransferase (APRT) deficiency is a rare , hereditary disorder characterized by renal excretion of 2,8-dihydroxyadenine (DHA), leading to kidney stone formation and chronic kidney disease (CKD). Treatment with a xanthine oxidoreductase inhibitor, allopurinol or febuxostat, reduces urinary DHA excretion and slows the progression of CKD. The method currently used for therapeutic
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Verification of chlorine exposure via LC-MS/MS analysis of base hydrolyzed chlorophenols from chlorotyrosine-protein adducts J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-05 Sharmin Sultana, Sarah Christeson, Mohamed Basiouny, Jacqueline Rioux, Livia Veress, Brian A. Logue
Inhalation of chlorine gas, with subsequent hydrolysis in the airway and lungs to form hydrochloric acid (HCl) and hypochlorous acid (HOCl), can cause pulmonary edema (i.e., fluid build-up in the lungs), pulmonary inflammation (with or without infection), respiratory failure, and death. The HOCl produced from chlorine is known to react with tyrosine to form adducts via electrophilic aromatic substitution
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Accelerated development of a SEC-HPLC procedure for purity analysis of monoclonal antibodies using design of experiments J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-05 Terezie Cernosek, Nitin Jain, Matthew Dalphin, Sue Behrens, Peter Wunderli
The complex structure of biopharmaceutical products poses an inherent need for their thorough characterization to ensure product quality, safety, and efficacy. Analytical size exclusion chromatography (SEC) is a widely used technique throughout the development and manufacturing of monoclonal antibodies (mAbs) which quantifies product size variants such as aggregates and fragments. Aggregate and fragment
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Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) methods for the therapeutic drug monitoring of cytotoxic anticancer drugs: An update J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-03 M. Briki, A. Murisier, M. Guidi, C. Seydoux, T. Buclin, C. Marzolini, F.R. Girardin, Y. Thoma, S. Carrara, E. Choong, L.A. Decosterd
In the era of precision medicine, there is increasing evidence that conventional cytotoxic agents may be suitable candidates for therapeutic drug monitoring (TDM)- guided drug dosage adjustments and patient’s tailored personalization of non-selective chemotherapies. To that end, many liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) assays have been developed for the quantification
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Analysis of free cisplatin in microdialysates and plasma ultrafiltrate by liquid chromatography-tandem mass spectrometry J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-03 A.V. Schmedes, C. Harlev, M. Bue, E.K. Petersen, M.L. Bergmann, L.K. Petersen, M. Stilling
Cisplatin is a potent cytotoxic agent used in the treatment of various malignancies and exerts its antitumor effect through malignant cell DNA damage and apoptosis induction. Evaluation of systemic delivery of cisplatin is important in optimization of cisplatin treatment. However, accurate quantification of systemic cisplatin is challenging due to its various forms in circulation. This study aimed
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Enhancing hyaluronidase enzyme activity: Insights from advancement in bovine and ovine testicular hyaluronidase purification J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-02 Seyed Mohammadhassan Modarressi, Zahra koolivand, Mojdeh Akbari
This essay investigates the use of an affinity resin named Capto lentil lectin for the purification of bovine and ovine testicular hyaluronidase. Hyaluronidase, an enzyme that degrades hyaluronic acid, is used widely in medical fields like dermatology, orthopedics, and ophthalmology. The research highlights the importance of optimizing the purification process to increase enzyme activity and purity
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Developing a detection strategy for ten paralytic shellfish poisonings in urine, combining high-throughput DESI-MS screening and accurate UPLC-QqQ/MS quantification J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-02 Jing Cai, Shiyan Li, Qingcheng Wang, Dan Deng, Shitong Wang, Lijun Ge, Yiwei Cui, Yuejian Shen, Qing Shen
Paralytic shellfish poisoning (PSP) is the most widespread and harmful form of shellfish poisoning with high mortality rate. In this study, a combined desorption electrospray ionization mass spectrometry (DESI-MS) and ultra-performance liquid chromatography triple quadrupole mass spectrometry (UPLC-QqQ/MS) method was established for the detection of PSPs in urine. The method was optimized using a spray
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High performance liquid chromatographic method optimized by Box-Behnken design model to determine caffeine in pharmaceutical preparations and urine samples J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-01 Syed Najmul Hejaz Azmi, Wafa Aqib Nasir Al Rawahi, Arwa Ibrahim Al Yahyai, Asma Ali Al Qasimi, Kothar Saif Al Fuliti, Omayma Said Al Qalhati, Nafisur Rahman, Qamar Uddin Ahmed
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An integrating strategy for systematic profiling of Chinese patent drug’s chemicalome and associated metabolome: Huanghou antidiarrhea dropping pills as a case study J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-01 Li Li, Xuguang Wang, Ruiting Ma, Mingxing Hou
Huanghou antidiarrhea dropping pills (HADP) is an efficient Chinese patent drug that is clinically used to treat diarrhea. However, its functional materials remain unclear due to the characteristics of traditional Chinese medicine, which is a multi-component and multi-target complex system. In this study, we investigated the intrinsic chemical components and combined with metabolism to reveal the functional
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Facial on-line enrichment of glycoproteins by capillary electrophoresis with boronate-functionalized poly(glycidyl methacrylate) microparticles coated column J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-26 Jian Zhang, Yanqing Miao, Hui Jing, Jingwen Wu, Chunye Liu
A facial and rapid method for glycoproteins enrichment by capillary electrophoresis was developed. The 3-aminophenylboronic acid-functionalized poly(glycidyl methacrylate) microparticles (PGMA@APBA) were attached to the capillary inlet (length of ∼1.5 cm) by electrostatic self-assemble action to prepare a partially coated capillary column. The process is simple and reversible, allowing for easy renewal