-
Properties of chromatographic columns prepared on the basis of poly(1-trimethylsilyl-1-propyne) modified with organic bases J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-16 Elena Y. Yakovleva, Yuri V. Patrushev, Inna K. Shundrina, Tatiana S. Glazneva
This article describes the effect of modification with organic bases such as uracil () and polyethyleneimine () on the adsorption and chromatographic properties of poly(1-trimethylsilyl-1-propyne) () used as a stationary phase () in packed and capillary columns. It was shown that the sorbents prepared on the basis of diatomite Chromosorb P NAW support and successively modified with 9 wt.% PTMSP and
-
Affinity purification/immobilization of poly histidine-tagged proteins by nickel-functionalized porous chitosan membranes J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-16 Zahra Hajihassan, Azadeh Ghaee, Parisa Bazargannia, Elahe Salmani Shahrivar
-
Enhancing quality evaluation in traditional Chinese medicine: Utilizing dual wavelength fusion fingerprint, electrochemical fingerprint, and DSC fingerprint J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-13 Ting Yang, Ming Chen, Xi Liu, Lan Xue, Yinlei Guo, Hong Zhang, Guoxiang Sun, Wanyang Sun
Developing a reliable and effective quality evaluation system for traditional Chinese medicine (TCM) is both challenging and crucial for its advancement. This study employs fingerprinting techniques to establish precise and comprehensive quality control for TCM, taking Xuezhikang capsules as an example and aiming to facilitate the internationalization of TCM. The “double wavelength absorption coefficient
-
Two-dimensional chiral metal-organic framework nanosheets L-hyp-Ni/Fe@SiO2 composite for HPLC separation J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-12 Ya-Lin Lai, Shun-Qiu Gao, Zhen Wang, Ke-Qian Yan, Bang-Jin Wang, Li-Ming Yuan
In this study, we have synthesised a chiral -hyp-Ni/Fe@SiO composite as a chiral stationary phase (CSP) for high-performance liquid chromatography (HPLC) for the first time. This was achieved by coating two-dimensional (2D) chiral metal-organic framework nanosheets (MONs) -hyp-Ni/Fe onto the surface of activated SiO microspheres using the “wrapped in net” method. The separation efficiency of the -hyp-Ni/Fe
-
Quantification of quaternary ammonium compounds by liquid chromatography-mass spectrometry: Minimizing losses from the field to the laboratory J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-11 Fanny Monteil-Rivera, Steven Locke, Mengwei Ye, Shirley Anne Smyth, Katrina Sullivan, Alexander Okonski, Magdalena Jagla, Don Gutzman
Quaternary Ammonium Compounds (QACs) are widely used in household, medical and industrial settings. As a consequence, they are ubiquitously found in the environment. Although significant efforts have been put into the development of sensitive and reproducible analytical methods, much less effort has been dedicated to the monitoring of QACs upon sample storage and sample preparation. Here we studied
-
Fabrication and application of a continuously regenerated cationic impurity removal device for ion chromatography J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-10 Yihua Li, Tianyang Zhang, Feifang Zhang, Weiqing Chen, Bingcheng Yang
A continuously regenerated cationic impurity removal device (CR-CRD) has been fabricated and applied for ion chromatography (IC). The removal of cationic impurities is realized by electrodialytically replacing the cationic impurities with hydronium ions. The device is configured in a sandwich structure and the central eluent channel is respectively isolated from both electrodes by stacked cation exchange
-
Theoretical and experimental study on the effect of scanning speed on FAIMS peaks J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-10 Jun Hu, Youjiang Liu, Jiao Jin, Han Wang, Shan Li, Chilai Chen
High-Field Asymmetric Ion Mobility Spectrometry (FAIMS) is a technique for ion separation and detection based on ion mobility variation under high electronic field. While compensation voltage scanning speed is a fundamental parameter in FAIMS, its impact on spectra remains unclear. In this work, a function referred to as F-EMG is introduced to describe the impact of compensation voltage scanning speed
-
Fluorine-functionalized magnetic amino microporous organic network for enrichment of perfluoroalkyl substances J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-10 Huipeng Sun, Yi Yang, Haofei Shen, Qilong Hao, Qin Huang, Jun Gao, Xiaoyan Liu, Haixia Zhang
Perfluoroalkyl substances (PFAS) are persistent organic pollutants that pose significant risks to human health and the environment. Efficient and selective enrichment of these compounds was crucial for their accurate detection and quantification in complex matrices. Herein, we report a novel magnetic solid-phase extraction (MSPE) method using fluorine-functionalized magnetic amino-microporous organic
-
A magnetic beads-based ligand fishing method for rapid discovery of monoterpene indoles as monoamine oxidase A inhibitors from Hunteria zeylanica J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-10 Huaixin Liu, Jincai Wang, Sirui Yang, Ziwei Li, Min Song, Xiaoqi Zhang, Jacques Crommen, Zhengjin Jiang, Tingting Zhang
In this study, a novel magnetic bead-based ligand fishing method was developed for rapid discovery of monoterpene indoles as monoamine oxidase A inhibitors from natural products. In order to improve the screening efficiency, two different magnetic beads, amine and carboxyl terminated magnetic beads, were comprehensively compared in terms of their ability to immobilize monoamine oxidase A (MAOA), biocatalytic
-
Preparing high-performance microspheres based on the chitosan-assisted dispersion of reduced graphene oxide in aqueous solution for bilirubin removal J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-10 Shanshan Wang, Yilin Yang, Haoqiu Chen, Qin Wang, Jiao Xie, Kaifeng Du
The removal of excess bilirubin from blood is of great clinical importance. Reduced graphene oxide (rGO) is often used to efficiently remove bilirubin. However, thin rGO pieces tend to aggregate in the aqueous phase because they are hydrophobic. In this context, we propose an effective strategy based on the chitosan-assisted (CS-assisted) dispersion of rGO to produce high-performance bilirubin-adsorbing
-
Establishment of a headspace-thermal desorption and gas chromatography-mass spectrometry method (HS-TD-GC-MS) for simultaneous detection of 51 volatile organic compounds in human urine: Application in occupational exposure assessment J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-10 Zhongheng Zhang, Qing Zhang, Ye Xi, Ying Zhou, Ming Zhan
-
Measuring aliphatic hydrocarbons in sediments by direct thermal desorption-gas chromatography-mass spectrometry: Matrix effects and quantification challenges J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-09 Florence Portet-Koltalo, Kévin Humbert, Julie Cosme, Maxime Debret, Christophe Morin, Steeven Le Gohlisse
Direct sample introduction thermal desorption (TD) coupled to GC–MS was investigated for the analysis of paraffinic hydrocarbons (HCs) from polluted sediments. TD-GC–MS is sometimes used for analysing paraffinic HCs from atmospheric particles but rarely for their direct desorption from sediments. So, the new TD methodology, applied to sediments, required development, optimization and validation. A
-
Exploring the effects of resin particle sizes on enhancing antibody binding capacity of a hybrid biomimetic ligand J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-09 Jiangping Yan, Dongqiang Lin, Shanjing Yao, Qilei Zhang
Particle size is a critical parameter of chromatographic resins that significantly affects protein separation. In this study, effects of resin particle sizes (31.26 μm, 59.85 μm and 85.22 μm named Aga-31, Aga-60 and Aga-85, respectively) on antibody adsorption capacity and separation performance of a hybrid biomimetic ligand were evaluated. Their performance was investigated through static adsorption
-
Adjustable chromatographic performance of silica-based mixed-mode stationary phase through the control of co-grafting amounts of imidazole and C18 chain J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-09 Yali Yang, Jia Chen, Xiaojing Liang, Bei Liu, Kaijun Quan, Xiuhui Liu, Hongdeng Qiu
In this paper, three imidazole- and C18- bifunctional silica stationary phases (Sil-Im-C18) were prepared by adjusting introduction interval of octadecyltrichlorosilane (ODS) and 3-imidazol-1-ylpropyl(trimethoxy)silane (TMPImS), which can be used for reversed-phase liquid chromatography (RPLC) and ion exchange chromatography (IEC) with adjustable performance. The successful preparation of Sil-Im-C18
-
A novel honeycomb-like 3D-printed device for rotating-disk sorptive extraction of organochlorine and organophosphorus pesticides from environmental water samples J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-08 Camila Scheid, Sofia Aquino Monteiro, Wendell Mello, Maiara Callegaro Velho, Juliana dos Santos, Ruy Carlos Ruver Beck, Monique Deon, Josias Merib
-
Computer-aided design space identification for screening of protein A affinity chromatography resins J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-08 Steven Sachio, Blaž Likozar, Cleo Kontoravdi, Maria M. Papathanasiou
The rapidly growing market of monoclonal antibodies (mAbs) within the biopharmaceutical industry has incentivised numerous works on the design of more efficient production processes. Protein A affinity chromatography is regarded as one of the best processes for the capture of mAbs. Although the screening of Protein A resins has been previously examined, process flexibility has not been considered to
-
Model-based design of gradient elution in liquid-liquid chromatography: Application to the separation of cannabinoids J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-07 Melanie Gerigk, Simon Vlad Luca, Sophie Schwarzenbach, Mirjana Minceva
Liquid-liquid chromatography (LLC) is a separation technique that utilizes a biphasic solvent system as the mobile and stationary phases. The components are separated solely due to their different distributions between the two liquid phases. Gradient change in the mobile phase composition during the chromatographic process is a powerful method for improving the resolution of separation or shortening
-
Comprehensive chromatographic assessment of forced degraded in vitro transcribed mRNA J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-06 Daniel M. Dayeh, Jaclyn Cika, Youmi Moon, Steven Henderson, Deanna Di Grandi, Yue Fu, Kathir Muthusamy, Nisha Palackal, Peter M. Ihnat, Erica A. Pyles
Heightened interest in messenger RNA (mRNA) therapeutics has accelerated the need for analytical methodologies that facilitate the production of supplies for clinical trials. Forced degradation studies are routinely conducted to provide an understanding of potential weak spots in the molecule that are exploited by stresses encountered during bulk purification, production, shipment, and storage. Consequently
-
Dimethylformamide dimethyl acetal reagent for in situ chiral analyses of organic molecules on Titan with the Dragonfly mass spectrometer space instrument (Dragonfly mission) J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-06 D. Boulesteix, A. Buch, J. Samson, C. Freissinet, D. Coscia, Y. He, S. Teinturier, J.C. Stern, M.G. Trainer, C. Szopa
Thanks to the space mission between 2004 and 2017, a lot was learned about Titan, the biggest satellite of Saturn, and its intriguing atmosphere, surface, and organic chemistry complexity. However, key questions about the potential for the atmosphere and surface chemistry to produce organic molecules of direct interest for prebiotic chemistry and life did not find an answer. Due to Titan potential
-
A mixed-mode reversed-phase/strong-anion-exchange stationary phase: Analyte-retention stability and application in the analysis of nonsteroidal anti-inflammatory drugs J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-05 Meng Xu, Zhenwei Wu
Mixed-mode reversed-phase/anion-exchange chromatography (RP/AEX) is an effective method for the chromatographic analysis of acidic drugs because it combines reversed-phase chromatography (RP) with anion-exchange chromatography (AEX). However, the result repeatability for the RP/AEX analysis of acidic drugs is frequently compromised by the detrimental effects of residual silanol groups in an RP/AEX
-
Corrigendum to “A specific nanobody-based affinity chromatography resin as a platform for small ubiquitin-related modifier fusion protein purification” [Journal of Chromatography A 1713 (2024) 464508] J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-05 Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
-
Purification of monoclonal antibodies using novel 3D printed ordered stationary phases J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-04 Mariachiara Conti, Deirdre Boland, Carmen Heeran, Jodie A. Symington, James R. Pullen, Simone Dimartino
3D printing offers the unprecedented ability to fabricate chromatography stationary phases with bespoke 3D morphology as opposed to traditional packed beds of spherical beads. The restricted range of printable materials compatible with chromatography is considered a setback for its industrial implementation. Recently, we proposed a novel ink that exhibits favourable printing performance (printing time
-
Fingerprinting of hydroxy propyl methyl cellulose by comprehensive two-dimensional liquid chromatography-mass spectrometry of monomers resulting from acid hydrolysis J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-04 Tijmen S. Bos, Bob W.J. Pirok, Leif Karlson, Staffan Schantz, Tina A. Dahlseid, Dwight R. Stoll, Govert W. Somsen
Hydroxypropyl methyl cellulose (HPMC) is a type of cellulose derivative with properties that render it useful in food, cosmetics, and pharmaceutical industry. The substitution degree and composition of the β-glucose subunits of HPMC affect its physical and functional properties, but HPMC characterization is challenging due to its high structural heterogeneity, including many isomers. In this study
-
Systematic evaluation of benzoylation for liquid chromatography-mass spectrometry analysis of different analyte classes J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-04 Ashley E. Lenhart, Peter-Philip M. Booth, Kaley M. Simcox, Brianna A. Ramos, Robert T. Kennedy
LC-MS is an indispensable tool for small molecule analysis in many fields; however, many small molecules require chemical derivatization to improve retention on commonly used reversed-phase columns and increase ionization. Benzoyl chloride (BzCl) derivatization is commonly used for derivatization of primary and secondary amines and phenolic alcohols, though evidence exists that with proper reaction
-
LC-MS/MS profiling of colon oxysterols and cholesterol precursors in mouse model of ulcerative colitis J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-04 Jiachun Lin, Xue Yang, Anhui Wang, Jinni Yang, Yuan Zheng, Haijuan Dong, Yuan Tian, Zunjian Zhang, Min Wang, Rui Song
Oxysterols and cholesterol precursors are being increasingly investigated in humans and laboratory animals as markers for various diseases in addition to their important functions. However, the quantitative analysis of these bioactive molecules is obstructed by high structural similarity, poor ionization efficiency and low abundance. The current assay methods are still cumbersome to be of practical
-
Preparation of surface molecularly imprinted polymers with Fe3O4/ZIF-8 as carrier for detection of Dimethoate in cabbage J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-04 Yanbo Tian, Abdul Majid, Yuewei Zhang, Liju Tan, Huiru Li, Na Wang, Jiangtao Wang
In this study, molecularly imprinted polymers (MIPs) were prepared for the specific recognition of organophosphorus pesticides and a rapid, efficient and simple method was established for the detection of dimethoate (DIT) in food samples. FeO magnetic nanoparticles were synthesized by co-precipitation, and FeO/ZIF-8 complexes were prepared by a modified in-situ polymerization method, and then magnetic
-
Optimization and uncertainty assessment of a gas chromatography coupled to Orbitrap mass spectrometry method to determine organic contaminants in blood: A case study of an endangered seabird J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-03 Bernat Oró-Nolla, Letizia Campioni, Silvia Lacorte
Birds are excellent bioindicators of environmental pollution, and blood provides information on contaminant exposure, although its analysis is challenging because of the low volumes that can be sampled. The objective of the present study was to optimize and validate a miniaturized and functional extraction and analytical method based on gas chromatography coupled to Orbitrap mass spectrometry (GCOrbitrap-MS)
-
Characterization of semi-packed columns with different cross section in high-pressure gas chromatography J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-03 Michel Rachkidi, Ambroisine Michel, Guy Raffin, Régis Barattin, Eric Colinet, Jérôme Randon
Hydrodynamics, efficiency, and loading capacity of two semi-packed columns with different cross sections (NANO 315 µm x 18 µm; CAP 1000 µm x 28 µm) and similar pillar diameter and pillar-pillar distance (respectively 5 µm and 2.5 µm) have been compared in high-pressure gas chromatography. A flow prediction tool has been first designed to determine pressure variations and hold-up time across the chromatographic
-
Design of smart temperature-sensitive terpolymeric hydrogel for multi-applications in liquid chromatography J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-02 Qian Zhao, Lulu Zhao, Yuefei Zhang, Wei Chen, Sheng Tang
Hydrogels with a unique three-dimensional network structure have been widely used in a variety of fields. However, hydrogels are prone to swelling under water-rich conditions, which severely limits their application in liquid chromatography. Therefore, producing a hydrogel with reliable performance and good mechanical property is essential. Smart temperature-sensitive chromatographic packings have
-
Determination of genotoxic impurities of aromatic aldehydes in pharmaceutical preparations by high performance liquid chromatography after derivatization with N-Cyclohexyl-4-hydrazino-1,8-naphthalenediimide J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-02 Zenghui Xu, Abudurexiti Reheman, Zhihao Lu, Shuiqiang Yu, Zhiwei Sun, Jinmao You
The detection of aromatic aldehydes, considered potential genotoxic impurities, holds significant importance during drug development and production. Current analytical methods necessitate complex pre-treatment processes and exhibit insufficient specificity and sensitivity. This study presents the utilization of naphthalenediimide as a pre-column derivatisation reagent to detect aromatic aldehyde impurities
-
Preparation and evaluation of a piperidinium-sulfonate based zwitterionic monolith for HILIC separation J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-02 Liang Lai, Mengyao Zhang, Tong Li, Jiahuan Qu, Dongsheng Xu, Pei Yu, Zhengjin Jiang
In this study, a novel piperidinium-sulfonate based zwitterionic hydrophilic monolith was prepared through thermally initiated -polymerization of a piperidinium-sulfonate monomer 3-(4-((methacryloyloxy)methyl)-1-methylpiperidin-1-ium-1-yl)propane-1-sulfonate (MAMMPS), and a hydrophilic crosslinker -methylenebisacrylamide (MBA) using -propanol and HO as porogenic system. Satisfactory mechanical and
-
Size exclusion chromatography of biopharmaceutical products: From current practices for proteins to emerging trends for viral vectors, nucleic acids and lipid nanoparticles J. Chromatogr. A (IF 4.1) Pub Date : 2024-04-01 Valentina D’Atri, Mateusz Imiołek, Colette Quinn, Abraham Finny, Matthew Lauber, Szabolcs Fekete, Davy Guillarme
The 21st century has been particularly productive for the biopharmaceutical industry, with the introduction of several classes of innovative therapeutics, such as monoclonal antibodies and related compounds, gene therapy products, and RNA-based modalities. All these new molecules are susceptible to aggregation and fragmentation, which necessitates a size variant analysis for their comprehensive characterization
-
Improving device design in insulator-based electrokinetic tertiary separations J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-31 Alaleh Vaghef-Koodehi, Patricia Cyr, Blanca H. Lapizco-Encinas
-
Preparation of high-efficiency HILIC capillary columns utilizing slurry packing at 2100 bar J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-30 Brady G. Anderson, Tate A. Hancock, Robert T. Kennedy
Complex mixture analysis requires high-efficiency chromatography columns. Although reversed phase liquid chromatography (RPLC) is the dominant approach for such mixtures, hydrophilic interaction liquid chromatography (HILIC) is an important complement to RPLC by enabling the separation of polar compounds. Chromatography theory predicts that small particles and long columns will yield high efficiency;
-
Can the heptapeptide ASSIVSF of the β2-adrenoceptor recognize ephedrine and pseudoephedrine epimers in a complex system? J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-30 Jiatai Yin, Yiheng Gou, Yiheng Wang, Qingyuan Ma, Rui Wang, Jing Yu, Yajun Zhang, Jing Wang, Qian Li, Xinfeng Zhao
Epimer separation is crucial in the field of analytical chemistry, separation science, and the pharmaceutical industry. No reported methods could separate simultaneously epimers or even isomers and remove other unwanted, co-existing, interfering substances from complex systems like herbal extracts. Herein, we prepared a heptapeptide-modified stationary phase for the separation of -(-)-ephedrine [(-)-Ephe]
-
Development of a chromatographic method for optimizing the thiol-maleimide coupling of polyoxometalate-polymer hybrids J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-30 Xiangqian Li, Lu Chen, Chengyang Hong, Wei Tian, Kun Yu, Hao Liu
-
Optimization, validation, and application of a liquid chromatography-tandem mass spectrometry method for the determination of 47 banned drug and related chemical residues in livestock urine using graphitized carboxyl multi-walled carbon nanotubes-based QuEChERS extraction J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-30 Youzhi Su, Jianjiang Lu, Jun Liu, Ning Wang, Fang Li, Hongqin Lei
The establishment of an efficient method for the analysis of drug residues in animal urine facilitates the real-time monitoring of drugs used in the production of animal-derived food. A modified QuEChERS extraction–liquid chromatography-tandem mass spectrometry (LC–MS/MS) method was established for the determination of 47 banned drug and related chemical residues in livestock urine. The sample was
-
An emission model for inhalable chemicals from children's play mats based on partition coefficients J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-30 Lihua Yu, Xiangke He, Cuiling Gao, Guixiao Li, Yue Wang, Yunbo Wang
-
Separation of inorganic anions on reversed-phase C18 columns with a phosphomolybdate mobile phase J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-29 Tomoko Kemmei, Azusa Yudo, Shuji Kodama, Atsushi Yamamoto, Yoshinori Inoue, Shigehiro Kagaya, Kazuichi Hayakawa
Reversed-phase high performance liquid chromatography (RP-HPLC) is the most widely used chromatographic method. In addition to hydrophobic interactions, additional interactions such as electrostatic interactions may participate in the retention behaviour of an analyte. This makes it possible to use RP-HPLC for many types of analyte. We describe a simple method for separating inorganic anions on a C18
-
Corrigendum to “Effect of cell shape on nonlinear electrophoresis migration” [Journal of Chromatography A, Volume 1717, 22 February 2024, 464685] J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-29 Viswateja Kasarabada, Olivia D. Ernst, Alaleh Vaghef-Koodehi, Blanca H. Lapizco-Encinas
-
Detection of trace components in Xiangdan injection of Dalbergia odorifera based on microextraction and back-extraction along with bar-form-diagram strategy J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-27 Yuan Chen, Yanqi Yang, Yingfeng Pan, Xiaoyi Liu, Hongxu Zhang, Yixia Pan, Jizhong Yan, Hui Zhang
Xiangdan Injection are commonly used traditional Chinese medicine formulations for the clinical treatment of cardiovascular diseases. However, the trace components of in Xiangdan Injection pose a challenge for evaluating its quality due to the difficulty of detection. This study proposes a technology combining dispersive liquid-liquid microextraction and back-extraction (DLLME-BE) along with Bar-Form-Diagram
-
m-nitrobenzyl alcohol supercharging reagent enhances the chromatographic separation and the charging of disulfide bond linked and His-tag peptides J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-27 Chia-wei Lin, Fabia Canonica, Simone Wüthrich, Antonia Fettelschoss-Gabriel, Ralph Schlapbach, Paolo Nanni
The linkages of disulfide bond (DSB) play important roles in protein stability and activity. Mass spectrometry-based (MS-based) techniques become accepted tools for DSB analysis in the recent decade. In the bottom-up approach, after enzyme digestion, the neighbouring amino acids of cysteines have great impacts on the physicochemical properties of resulting disulfide bond peptides, determining their
-
Rapid determination of glyphosate and glufosinate in human blood by probe electrospray ionization tandem mass spectrometry J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-27 Jiahua Ji, Yunfeng Zhang, Bo Zou, Linpei Dong, Aihua Wang, Xiaojun Wu, Hong Zhou, Zhenhe Chen, Ying Zhang, Yao Liu
In forensic science, glyphosate (GLYP) and glufosinate (GLUF), a class of non-selective broad-spectrum herbicides, have been frequently encountered in many fatal poisoning and suicide cases due to their widespread availability. Therefore, it is essential to develop an effective method for detecting these compounds. Some conventional methods, such as gas chromatography–mass spectrometry (GC–MS) or liquid
-
Porous covalent organic framework nanofibrous membrane for excellent enrichment and ultra-high sensitivity detection of trace organochlorine pesticides in water J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-27 Chunxiang Lin, Yufang Weng, Yule Lin, Yifan Liu, Xiaojuan Li, Yuancai Lv, Xiaoxia Ye, Liang Song, Guifang Yang, Minghua Liu
Developing adsorbents with high performance and long service life for effective extracting the trace organochlorine pesticides (OCPs) from real water is attracting numerous attentions. Herein, a self-standing covalent organic framework (COF-TpPa) membrane with fiber morphology was successfully synthesized by using electrospun nanofiber membranes as template and employed as solid-phase microextraction
-
Affinity chromatography for virus-like particle manufacturing: Challenges, solutions, and perspectives J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-27 Jing Ma, Zengquan Tian, Qinghong Shi, Xiaoyan Dong, Yan Sun
The increasing medical application of virus-like particles (VLPs), notably vaccines and viral vectors, has increased the demand for commercial VLP production. However, VLP manufacturing has not yet reached the efficiency level achieved for recombinant protein therapeutics, especially in downstream processing. This review provides a comprehensive analysis of the challenges associated with affinity chromatography
-
Assessment of liquid-liquid partition for the assignment of descriptors for the solvation parameter model J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-27 Colin F. Poole
The solvation parameter model uses five system independent descriptors to characterize compound properties defined as excess molar refraction, E, dipolarity/polarizability, S, hydrogen-bond acidity, A, hydrogen-bond basicity, B, and McGowan's characteristic volume, V, to model transfer properties between condensed phases. The V descriptor is assigned from structure. For compounds liquid at 20 °C the
-
Room-temperature fabrication of fluorinated covalent organic polymer @ Attapulgite composite for in-syringe membrane solid-phase extraction and analysis of domoic acid in aquatic products J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-26 Simin Huang, Xiaoqian Wang, Bo Zhang, Ling Xia, Yi Chen, Gongke Li
A novel fluorinated covalent organic polymer @ attapulgite composite (F-COP@ATP) was prepared at room temperature for in-syringe membrane solid-phase extraction (SM-SPE) of domoic acid (DA) in aquatic products. Natural ore ATP has the advantages of low cost, good mechanical strength and abundant hydroxyl group on its surface, and modified F-COP layer can provide abundant adsorption sites. F-COP@ATP
-
The best structures of LC columns—A theoretical perspective J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-26 Leonid M. Blumberg
The largest () that LC analysis can generate in isocratic or gradient elution analysis of a given sample in a given time at a given pressure is proportional to the () of its column structure. In this study, the multi-channel structures with s (OPPC) and (OCC) where compared with PC2 – a typical core-shell packed with 2 μm particles. These columns have of 1.27, 1.17 and 0.41, respectively. The former
-
Retention behavior of nucleosides and nucleobases on a 3 μm undecylenic acid-functionalized silica column in per aqueous liquid chromatography and hydrophilic interaction liquid chromatography separation modes J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-26 Xiao-Dong Cheng, Zheng Zhang, Xue-xin Dai, Yun-Ping Li
-
Separation of the diastereomers of phosphorothioated siRNAs by anion-exchange chromatography under non-denaturing conditions J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-25 Hiroyuki Togawa, Takashi Okubo, Kazuki Horiuchi, Takao Yamaguchi, Elisa Tomita-Sudo, Tomoka Akita, Junji Kawakami, Satoshi Obika
In recent years, several small interfering RNA (siRNA) therapeutics have been approved, and most of them are phosphorothioate (PS)-modified for improving nuclease resistance. This chemical modification induces chirality in the phosphorus atom, leading to the formation of diastereomers. Recent studies have revealed that p and p configurations of PS modifications of siRNAs have different biological properties
-
Improved assay development of pharmaceutical modalities using feedback-controlled liquid chromatography optimization J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-24 Fatima Naser Aldine, Andrew N. Singh, Heather Wang, Devin M. Makey, Rodell C. Barrientos, Michelle Wong, Pankaj Aggarwal, Erik L. Regalado, Imad A. Haidar Ahmad
Development of meaningful and reliable analytical assays in the (bio)pharmaceutical industry can often be challenging, involving tedious trial and error experimentation. In this work, an automated analytical workflow using an AI-based algorithm for streamlined method development and optimization is presented. Chromatographic methods are developed and optimized from start to finish by a feedback-controlled
-
Establishment of cluster of differentiation 20 immobilized cell membrane chromatography for the screening of active antitumor components in traditional Chinese medicine J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-24 Yifan Ding, Na Guo, Yuhan Jiang, Sihan Liu, Tongpei Zhou, Haoyun Bai, Yanni Lv, Shengli Han, Langchong He
Non-Hodgkin lymphoma (NHL) is a heterogeneous group of malignant tumors occurring in B or T lymphocytes, and no small molecule-positive drugs to treat NHL have been marketed. Cluster of differentiation 20 (CD20) is an important molecule regulating signaling for the life and differentiation of B lymphocytes and possesses the characteristics of a drug target for treating NHL. 2-Methoxyestradiol induces
-
Sacrificial template synthesis of hollow sulfonate group functionalized microporous organic network for efficient solid phase extraction of sulfonamide antibiotics from milk and honey samples J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-23 Shuo-Wen Gao, Li-Hua Chen, Yuan-Yuan Cui, Cheng-Xiong Yang
The highly conjugated and hydrophobic characteristics of microporous organic networks (MONs) have largely impeded their broad applications in sample pretreatment especially for the polar or ionic analytes. In this work, a novel uniform hollow shaped sulfonate group functionalized MON (H-MON-SOH-2) was synthesized via the sacrificial template method for the efficient solid phase extraction (SPE) of
-
Advances in Multidimensional Chromatography J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-22 Paola Dugo, Francesco Cacciola, Chiara Cordero
-
In-silico method development and optimization of on-line comprehensive two-dimensional liquid chromatography via a shortcut model J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-21 Monica Tirapelle, Dian Ning Chia, Fanyi Duanmu, Maximilian O. Besenhard, Luca Mazzei, Eva Sorensen
Comprehensive two-dimensional liquid chromatography (LCxLC) represents a valuable alternative to conventional single column, or one-dimensional, liquid chromatography (1D-LC) for resolving multiple components in a complex mixture in a short time. However, developing LCxLC methods with trial-and-error experiments is challenging and time-consuming, which is why the technique is not dominant despite its
-
Multistep pH-peak-focusing liquid chromatography with a hydrophilic polymer gel column for separation of rare earth elements J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-20 Masami Shibukawa, Yosuke Onoyama, Yuiko Handa-Tasaki, Shingo Saito
Multistep pH-peak-focusing liquid chromatography with a column packed with a hydrophilic polymer gel (a cross-linked hydroxylated methacrylic polymer gel) was developed for separation of rare earth metal ions. Metal ions in a sample solution introduced to the column are chromatographically extracted into the stationary gel phase at the top of the column equilibrated with a basic solution used as the
-
Isomers-oriented separation of forty-five plasma bile acids with liquid chromatography-tandem mass spectrometry J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-20 Lei Zhang, Xu Liu, Tenghui Jin, Jing Dong, Xiaodong Li, Youyi Zhang, Dongyang Liu
Some bile acids (BAs) were considered as biomarkers or have therapeutical effect on metabolic diseases. However, due to the existence of isomers and limitations in sensitivity, simultaneous quantification of multiple BAs remains a challenge. The aim of this study is to establish an accurate and sensitive method for the determination of multiple BAs with similar polarity. A LC-MS/MS analytical method
-
Development, characterization and application of 3D printed adsorbents for in situ recovery of taxadiene from microbial cultivations J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-19 Giuseppe Rafael Galindo-Rodriguez, M. Sulaiman Sarwar, Leonardo Rios-Solis, Simone Dimartino
Microbial cell factories are an attractive alternative to produce high-value natural products using sustainable processes. However, product recovery is one of the main challenges to reduce production cost and make these technologies economically interesting. In this work, new resins were formulated to 3D print hydrophobic adsorbents for the recovery of biologics from microbial cultivations. Benzyl
-
On the contribution of the top and bottom walls in micro-pillar array columns and related high-aspect ratio chromatography systems J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-19 Ali Moussa, Bram Huygens, Claudia Venditti, Alessandra Adrover, Gert Desmet
We report on a steady-state based, and hence highly accurate numerical modelling study of the effect of the top and bottom wall in the current generation of micro-pillar array columns. These have a mesoporous retention layer that not only covers the pillar walls but also the bottom wall. Our results show that the performance of these columns can in general not be improved by also covering the top wall
-
A solid-liquid microextraction approach from a spot in an adsorbent layer of a chromatographic plate J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-18 Rafał Gajos, Anna Klimek-Turek, Tadeusz H. Dzido
A new approach to extracting substances from a spot on a chromatographic plate for subsequent liquid chromatography-mass spectrometry analysis is described. This method involves extraction in a solid phase (an adsorbent layer of a chromatographic plate) - a liquid system using a simple device. For a single extraction of six selected coccidiostats from the adsorbent layer on the chromatographic plate